Results 31 to 40 of about 3,939,990 (297)
Arguments for Human Therapeutic Cloning
Biomolecules & Biomedicine, 2008 For over 30 years, many Western governments have regulated scientific research involving human subjects. According to Knoppers implementation of regulation followed a long and checkered history of research abuse.
Krešimir Pavelić
doaj +1 more source
Molecular cloning and physical mapping of murine cytomegalovirus DNA [PDF]
, 1983 Murine cytomegalovirus (MCMV) Smith strain DNA is cleaved by restriction endonuclease HindIII into 16 fragments, ranging in size from 0.64 to 22.25 megadaltons.
Ebeling, Angelika +3 more
core +1 more source
Molecular cloning of cat interleukin-12 [PDF]
Immunogenetics, 1997 The recently discovered interleukin-12 (IL-12) is a heterodimeric 70000 Mr cytokine composed of a disulphide-bonded 35000 Mr (p35) subunit and a 40000 Mr (p40) subunit. Produced mainly by activated monocytes/macrophages and B cells, it enhances NK/LAK cell cytolytic activity and stimulates cytotoxic T-cell responses and IFN-γ production.
Horzinek, M.C. +3 more
openaire +3 more sources
Recombination-assisted megaprimer (RAM) cloning
MethodsX, 2014 No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive. Restriction-free cloning is among the simplest, most rapid, and cost-effective methods, but does not always provide ...
Jacques Mathieu +2 more
doaj +1 more source
Molecular cloning of duck CD40 and its immune function research
Poultry Science, 2021 : Cosignal molecules are cell surface molecules that transduce signals to other cells to modulate immune response positively (costimulate) or negatively (cosuppress).
Rujuan Zhang +19 more
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Molecular cloning and characterization of PLC‐η2 [PDF]
The FASEB Journal, 2005 Phospholipase C isozymes catalyze the conversion of phosphatidylinositol‐(4,5)P 2 to the Ca 2+ ‐mobilizing second messenger, Ins(1,4,5)P 3 , and the protein kinase C‐activating second messenger, diacylglycerol ...
Yixing, Zhou +3 more
openaire +2 more sources
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
BMC Research Notes, 2012 Background Molecular cloning of DNA fragments >5 kbp is still a complex task. When no genomic DNA library is available for the species of interest, and direct PCR amplification of the desired DNA fragment is unsuccessful or results in an incorrect ...
Orlova Nadezhda A +2 more
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From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source