Results 31 to 40 of about 485,373 (308)
iScience, 2019 Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning.
Bryan Bishé +2 more
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HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review
Biomedicines, 2021 Oxygen is essential for the maintenance of the body. Living organisms have evolved systems to secure an oxygen environment to be proper. Hypoxia-inducible factor (HIF) plays an essential role in this process; it is a transcription factor that mediates ...
Kiichi Hirota
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BioTechniques, 2019 Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Jens Staal +4 more
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BioTechniques, 2020 During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited.
Tomo Kondo, Shigehiko Yumura
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Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. [PDF]
PLoS ONE, 2013 The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites.
Stephan Kirchmaier +2 more
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Optimal cloning of PCR fragments by homologous recombination in Escherichia coli. [PDF]
PLoS ONE, 2015 PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown
Ana Paula Jacobus, Jeferson Gross
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A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
BMC Biotechnology, 2010 Background The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences.
Nørholm Morten HH
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Metabolic Engineering Communications, 2016 Molecular Cloning Designer Simulator (MCDS) is a powerful new all-in-one cloning and genetic engineering design, simulation and management software platform developed for complex synthetic biology and metabolic engineering projects.
Zhenyu Shi, Claudia E. Vickers
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Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice
Czech Journal of Genetics and Plant Breeding, 2022 Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism,
Yu Li +6 more
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In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning [PDF]
, 2019 12 pags., 5 figs.Molecular cloning is a cornerstone of biomedical, biotechnological, and synthetic biology research. As such, improved cloning methodologies can significantly advance the speed and cost of research projects.
Watson, Jake F. +2 more
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