Results 51 to 60 of about 486,356 (308)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Molecular cloning of schistosome genes

Parasitology, 1986
As part of an integrated programme investigating human schistosomiasis, work which involves epidemiological surveys and detailed immunological studies as well as biochemical investigations, we have, over the last three years, been cloning schistosome genes in a variety of plasmid and lambda vector systems.
D W, Taylor, J S, Cordingley, D W, Dunne, K S, Johnson, W J, Haddow, C E, Hormaeche, V, Nene, A E, Butterworth   +7 more
openaire   +2 more sources

Molecular cloning, functional expression and characterisation of RCC reductase involved in chlorophyll catabolism [PDF]

, 2000
Red chlorophyll catabolite (RCC) reductase (RCCR) and pheophorbide (Pheide) a oxygenase (PaO) catalyse the key reaction of chlorophyll catabolism, porphyrin macrocycle cleavage of Pheide a to a primary fluorescent catabolite (pFCC).
Hortensteiner, S., Donnison, Iain S., Hunziker, P. E., Wuthrich, K. L., Bovet, L., Donnison, I. S., Hörtensteiner, Stefan, Bovet, Lucien, Wüthrich, Karin L., Hunziker, Peter E.   +9 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

BMC Research Notes, 2012
Background Molecular cloning of DNA fragments >5 kbp is still a complex task. When no genomic DNA library is available for the species of interest, and direct PCR amplification of the desired DNA fragment is unsuccessful or results in an incorrect ...
Orlova Nadezhda A, Orlov Alexandre V, Vorobiev Ivan I   +2 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment

Drug Design, Development and Therapy, 2020
Hassan Dana, 1, 2,* Ghanbar Mahmoodi Chalbatani, 1,* Elahe Gharagouzloo, 1 Seyed Rouhollah Miri, 1 Fereidoon Memari, 1 Reza Rasoolzadeh, 3 Mohammad Reza Zinatizadeh, 3 Peyman Kheirandish Zarandi, 3 Vahid Marmari 2 1Cancer Research Center, Cancer ...
Dana H, Mahmoodi Chalbatani G, Gharagouzloo E, Miri SR, Memari F, Rasoolzadeh R, Zinatizadeh MR, Kheirandish Zarandi P, Marmari V   +8 more
doaj  

The TelN/tos-assisted precise targeting of chromosome segments (TAPE)

Journal of Advanced Research, 2022
Introduction: Performing genomic large segmentation experiments will promote the annotation of complex genomic functions and contribute to the synthesis of designed genomes.
You-Zhi Cui, Jian-Ting Zhou, Bing-Zhi Li, Ying-Jin Yuan   +3 more
doaj   +1 more source

Cloning, sequencing, and overexpression of gene 16 of salmonella bacteriophage P22 [PDF]

, 1992
Umlauf B, Dreiseikelmann B. Cloning, sequencing, and overexpression of gene 16 of salmonella bacteriophage P22. Virology. 1992;188(2):495-501.It has been suggested that gene product 16 of bacteriophage P22 forms a pore for DNA transfer and/or that it ...
Dreiseikelmann, Brigitte, Umlauf, Bettina   +1 more
core   +2 more sources

Table_1_Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning.DOCX [PDF]

, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Yanjing Zeng (8300250), Pu Yan (146101), Wentao Shen (161217), Peng Zhou (116747), Xiaoying Li (147528), Decai Tuo (8300253)   +5 more
core   +1 more source