Results 51 to 60 of about 485,373 (308)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment

Drug Design, Development and Therapy, 2020
Hassan Dana, 1, 2,* Ghanbar Mahmoodi Chalbatani, 1,* Elahe Gharagouzloo, 1 Seyed Rouhollah Miri, 1 Fereidoon Memari, 1 Reza Rasoolzadeh, 3 Mohammad Reza Zinatizadeh, 3 Peyman Kheirandish Zarandi, 3 Vahid Marmari 2 1Cancer Research Center, Cancer ...
Dana H, Mahmoodi Chalbatani G, Gharagouzloo E, Miri SR, Memari F, Rasoolzadeh R, Zinatizadeh MR, Kheirandish Zarandi P, Marmari V   +8 more
doaj  

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli

, 1989
Friehs K, Bailey JE. Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology. 1989;9(4):305-316.The plasmid pJMC40 containing the lon gene was transformed into E.
Bailey, J. E., Friehs, Karl
core   +1 more source

Molecular cloning using in vivo DNA assembly [PDF]

, 2023
Here we describe the in vivo DNA assembly approach, where molecular cloning procedures are performed using an E. coli recA-independent recombination pathway, which assembles linear fragments of DNA with short homologous termini.
Arroyo-Urea, Sandra, Watson, Jake F., García-Nafría, Javier   +2 more
core   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Molecular cloning of the chicken avidin cDNA [PDF]

Nucleic Acids Research, 1987
A cDNA for chicken avidin was identified in a chicken oviduct cDNA library by screening with antibodies and synthetic oligodeoxyribonucleotides. Four recombinant clones were characterized and each contained the sequence of the oligonucleotide probes used in screening.
M L, Gope, R A, Keinänen, P A, Kristo, O M, Conneely, W G, Beattie, T, Zarucki-Schulz, B W, O'Malley, M S, Kulomaa   +7 more
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

BMC Biotechnology, 2011
Background Although a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process. Results Here we report a highly simplified, reliable, and efficient PCR-based cloning technique to insert any ...
Lu Jia, Shen Benchang, Wen Aiyun, Li Chaokun, Huang Yao, Chang Yongchang   +5 more
doaj   +1 more source

Molecular cloning of CoA synthase - The missing link in CoA biosynthesis [PDF]

, 2002
Coenzyme A functions as a carrier of acetyl and acyl groups in living cells and is essential for numerous biosynthetic, energy-yielding, and degradative metabolic pathways. There are five enzymatic steps in CoA biosynthesis. To date, molecular cloning of
Wang, ML, Naidenov, V, Vudmaska, M, Kukharenko, O, Fenton, TR, Gout, IT, Palchevskii, S, Ovcharenko, G, Rebholz, H, Filonenko, V, Savinska, L, Matsuka, G, Babich, A, Verdier, F, Pobigailo, N, Nemazanyy, I, Shepherd, P, Zhyvoloup, A, Valovka, T, Panasyuk, G   +19 more
core   +1 more source