Results 51 to 60 of about 805,120 (315)

A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

BMC Research Notes, 2012
Background Molecular cloning of DNA fragments >5 kbp is still a complex task. When no genomic DNA library is available for the species of interest, and direct PCR amplification of the desired DNA fragment is unsuccessful or results in an incorrect ...
Orlova Nadezhda A, Orlov Alexandre V, Vorobiev Ivan I   +2 more
doaj   +1 more source

The TelN/tos-assisted precise targeting of chromosome segments (TAPE)

Journal of Advanced Research, 2022
Introduction: Performing genomic large segmentation experiments will promote the annotation of complex genomic functions and contribute to the synthesis of designed genomes.
You-Zhi Cui, Jian-Ting Zhou, Bing-Zhi Li, Ying-Jin Yuan   +3 more
doaj   +1 more source

Efficient Parallel Algorithm for Statistical Ion Track Simulations in Crystalline Materials

, 2008
We present an efficient parallel algorithm for statistical Molecular Dynamics simulations of ion tracks in solids. The method is based on the Rare Event Enhanced Domain following Molecular Dynamics (REED-MD) algorithm, which has been successfully applied
Beardmore, Beardmore, Biersack, Brandt, Byoungseon Jeon, Cai, Cai, Elteckov, Firsov, Gropp, Kang, Kishinevskii, Niels Grønbech-Jensen, Robinson, Sillanpää, Tasch, Van Brutzel, Verlet, Ziegler, Ziegler   +19 more
core   +1 more source

Debating whether Dinosaurs Should be "Cloned" from Ancient DNA to Promote Cooperative Learning in an Introductory Evolution Course [PDF]

, 2001
This interactive internet exercise engages students in cooperative library and web research on a controversial topic in science (and ethics), specifically the cloning of extinct forms of life.
Constance Soja, Deborah Huerta
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

The Alliance for Cellular Signaling Plasmid Collection: A Flexible Resource for Protein Localization Studies and Signaling Pathway Analysis [PDF]

, 2007
Cellular responses to inputs that vary both temporally and spatially are determined by complex relationships between the components of cell signaling networks.
Bryan, Heather, Chandy, Grischa, Chang, Mi Sook, Coutre, Sam, Dhandapani, Kavitha, Driver, Adrienne, Fraser, Iain D. C., Fung, Eileen, Gehrig, Elizabeth, Kivork, Christine, Lee, Genie, Liu, Jamie, Lyon, Stephen, Maer, Andreia, Meyer, Tobias, Mi, Qingli, Ning, Yuhong, O'Rourke, Nancy, Park, Wei Sun, Randhawa, Baljinder, Santat, Leah, Saunders, Brian, Simon, Melvin I., Subramaniam, Shankar, Verghese, Mary, Wall, Estelle, Whalen, James, Zavzavadjian, Joelle R., Zhu, Xiaocui   +28 more
core   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

BMC Biotechnology, 2011
Background Although a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process. Results Here we report a highly simplified, reliable, and efficient PCR-based cloning technique to insert any ...
Lu Jia, Shen Benchang, Wen Aiyun, Li Chaokun, Huang Yao, Chang Yongchang   +5 more
doaj   +1 more source