Results 71 to 80 of about 485,373 (308)
Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations
FEBS Letters, EarlyView.The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas +6 more
wiley +1 more source
Molecules, 2018 Cloning and expression plasmids are the workhorses of modern molecular biology. Despite the pathway paved by synthetic biology, laboratories around the globe still relay on standard cloning techniques using plasmids with reporter proteins for positive ...
Margarita Daniela Tafoya-Ramírez +7 more
doaj +1 more source
Molecular Cloning of Human and Murine hsp60 Related Sequences [PDF]
, 1990 Full length P1 eDNA clones have been obtained from human and CHO sources (Jindal et al., 1989; Picketts et al., 1989) which contain sequences that show extensive sequence and structural similarity to the chaperonin family of proteins, including the ...
Venner, Thomas
core
Molecular cloning of Anuroctoxin gene into pPICZαA: A yeast expression vector
, 2021 Anuroctoxin gene was amplified from source plasmid and cloned into pPICZαA vector using the restriction ligation approach. The recombinant vector was transformed into bacterial strain E. coli using heat shock method.
Gembiczki, Júlia
core
FEBS Letters, EarlyView.An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley +1 more source
BMC Biotechnology, 2011 Background RNA interference (RNAi) is a conserved gene silencing mechanism mediated by small inhibitory microRNAs (miRNAs). Promoter-driven miRNA expression vectors have emerged as important tools for delivering natural or artificially designed miRNAs to
Garwick-Coppens Sara E +2 more
doaj +1 more source
The sarcolemmal calcium pump inhibits the calcineurin/nuclear factor of activated T-cell pathway via interaction with the calcineurin A catalytic subunit [PDF]
, 2005 The calcineurin/nuclear factor of activated T-cell (NFAT) pathway represents a crucial transducer of cellular function. There is increasing evidence placing the sarcolemmal calcium pump, or plasma membrane calcium/calmodulin ATPase pump (PMCA), as a ...
Rodriguez, Antonio +21 more
core +1 more source
Ubiquitination of secretory granules promotes their crinophagic degradation in Drosophila
FEBS Letters, EarlyView.Ubiquitination of secretory granules in Drosophila larval salivary glands is a critical molecular trigger for crinophagy, the lysosomal degradation of unreleased, or low‐quality granules. The E3 ubiquitin ligase Cnot4 is recruited to the surface of secretory granules to induce crinophagy.
Tamás Csizmadia +6 more
wiley +1 more source
Bacterial diversity of symptomatic primary endodontic infection by clonal analysis
Brazilian Oral ResearchThe aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and ...
Letícia Maria Menezes NÓBREGA +4 more
doaj +1 more source
Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment
Molecular Oncology, EarlyView.This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley +1 more source