Results 81 to 90 of about 486,356 (308)
Molecules, 2018 Cloning and expression plasmids are the workhorses of modern molecular biology. Despite the pathway paved by synthetic biology, laboratories around the globe still relay on standard cloning techniques using plasmids with reporter proteins for positive ...
Margarita Daniela Tafoya-Ramírez +7 more
doaj +1 more source
Molecular Cloning of Human and Murine hsp60 Related Sequences [PDF]
, 1990 Full length P1 eDNA clones have been obtained from human and CHO sources (Jindal et al., 1989; Picketts et al., 1989) which contain sequences that show extensive sequence and structural similarity to the chaperonin family of proteins, including the ...
Venner, Thomas
core
Molecular cloning and sequencing analysis differentially expressed TDFs. [PDF]
, 2013 Molecular cloning and sequencing analysis differentially expressed TDFs.
Xuemin Wang (337948) +3 more
core +1 more source
FEBS Letters, EarlyView.Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley +1 more source
Resistance and susceptibility to late blight in Solanum: gene mapping, cloning and stacking [PDF]
, 2010 The potato late blight disease, caused by the oomycete Phytophthora infestans, is a major threat for potato production worldwide. To breed potato varieties with durable resistance against P. infestans, it is necessary to combine two or more resistance (R)
Verzaux, E.C.
core
BMC Biotechnology, 2011 Background RNA interference (RNAi) is a conserved gene silencing mechanism mediated by small inhibitory microRNAs (miRNAs). Promoter-driven miRNA expression vectors have emerged as important tools for delivering natural or artificially designed miRNAs to
Garwick-Coppens Sara E +2 more
doaj +1 more source
The sarcolemmal calcium pump inhibits the calcineurin/nuclear factor of activated T-cell pathway via interaction with the calcineurin A catalytic subunit [PDF]
, 2005 The calcineurin/nuclear factor of activated T-cell (NFAT) pathway represents a crucial transducer of cellular function. There is increasing evidence placing the sarcolemmal calcium pump, or plasma membrane calcium/calmodulin ATPase pump (PMCA), as a ...
Rodriguez, Antonio +21 more
core +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Bacterial diversity of symptomatic primary endodontic infection by clonal analysis
Brazilian Oral ResearchThe aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and ...
Letícia Maria Menezes NÓBREGA +4 more
doaj +1 more source
FEBS Letters, EarlyView.The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile +8 more
wiley +1 more source