Results 101 to 110 of about 805,120 (315)

‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes

Current Issues in Molecular Biology
To clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. Some cloning vectors have been developed for the cloning of PCR products, including blunt-end and T-A cloning. However, different plasmids are required
Xingli Zhang, Chong Teng, Kaidi Lyu, Shanhua Lyu, Yinglun Fan   +4 more
doaj   +1 more source

Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli

Plant Methods, 2005
Plant genome sequence data now provide opportunities to conduct molecular genetic studies at the level of the whole gene locus and above. Such studies will be greatly facilitated by adopting and developing further the new generation of genetic ...
Göttgens Berthold, Roden Laura C, Mutasa-Göttgens Effie S   +2 more
doaj   +1 more source

BacS: An Abundant Bacteroid Protein in \u3cem\u3eRhizobium etli\u3c/em\u3e Whose Expression Ex Planta Requires \u3cem\u3enifA\u3c/em\u3e [PDF]

, 2003
Rhizobium etli CFN42 bacteroids from bean nodules possessed an abundant 16-kDa protein (BacS) that was found in the membrane pellet after cell disruption. This protein was not detected in bacteria cultured in tryptone-yeast extract.
Davila, Guillermo, Jahn, Olivia Jeanne, Noel, K. Dale, Romero, David   +3 more
core   +1 more source

Transcription of the sporulation gene ssgA is activated by the IcIR-type regulator SsgR in a whi-independent manner in Streptomyces coelicolor A3(2). [PDF]

, 2004
SsgA plays an important role in the control of sporulation-specific cell division and morphogenesis of streptomycetes, and ssgA null mutants have a rare conditionally non-sporulating phenotype.
Bentley, Bierman, Chater, Chater, Chater, Chater, Chater, Embley, Flärdh, Flärdh, Flärdh, Galinier, Higgins, Hindle, Horinouchi, Ikeda, Ishikawa, Janssen, Jiang, Kawamoto, Kawamoto, Keijser, Kelemen, Kieser, Larson, Lydiate, MacNeil, Ohnishi, Pan, Ryding, Sambrook, Soliveri, Strohl, Sunnarborg, Takano, Takano, Van Wezel, Van Wezel, Van Wezel, Vara, Yamamoto, Yamazaki, Zhang   +42 more
core   +2 more sources

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Cloning alphavirus and flavivirus sequences for use as positive controls in molecular diagnostics

Revista Peruana de Medicina Experimental y Salud Pública, 2016
The purpose of the study was to obtain a positive control to validate molecular techniques (reverse transcription– polymerase chain reaction [RT-PCR]) used in the diagnosis and research of viral infections. From strains of Chikungunya virus (CHIKV), Zika
Daría Camacho, Jesús Reyes, Leticia Franco, Guillermo Comach, Elizabeth Ferrer   +4 more
doaj   +1 more source

Molecular cloning of mouse PSP mRNA

Nucleic Acids Research, 1985
PSP is the most abundant translation product of mouse parotid glands where its production is co-ordinated with that of salivary amylase. The synthesis of these two proteins apparently is restricted to this tissue. In order to enable us to study common regulatory elements in the genes of the two proteins, double stranded cDNA, synthesized for parotid ...
H O, Madsen, J P, Hjorth
openaire   +3 more sources

Creating E.coli Library of Diagnostic Gene for Influenza B Virus Using Molecular Cloning Technique [PDF]

, 2014
Influenza viruses cause seasonal epidemics associated with high morbidity, especially in immunocompromised and extreme age group individuals. Many strategies for combating these viruses are in practice; however, developing and maintaining sensitive ...
Chinbat, Oyunsuvd
core  

Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences [PDF]

, 2001
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, A.G.C.L., Kowalchuk, G.A., Jong, S., de, Kline, E., Stephen, J.R., Laanbroek, H.J.   +5 more
core   +3 more sources

Circular RNA expression landscapes in myelodysplastic neoplasms: Associations with mutational signatures and disease progression

Molecular Oncology, EarlyView.
In this explorative study, the abundance of circular RNA molecules in bone marrow stem cells was found to be elevated in patients with high‐risk myelodysplastic neoplasms, and to be associated with an increased risk of progression to acute myeloid leukemia.
Eileen Wedge, Morten Tulstrup, Gabriele Todisco, Pedro Moura, Christophe Côme, Jakob Werner Hansen, Jakob Schmidt Jespersen, Balthasar Schlotmann, Maria Creignou, Mette Dahl, Claudia Schöllkopf, Klas Raaschou‐Jensen, Krister Wennerberg, Bo Porse, Joachim Weischenfeldt, Eva Hellström‐Lindberg, Lasse Sommer Kristensen, Kirsten Grønbæk   +17 more
wiley   +1 more source