Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis
Scientific ReportsThis study introduces a novel cost-effective technique for cloning of linear DNA plasmid inserts, aiming to address the associated expenses linked with popular in vitro DNA assembly methods.
Petra Paclikova, Jakub Harnos
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Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera). [PDF]
BMC Plant Biol, 2020 Wang G, Liang YH, Zhang JY, Cheng ZM.
europepmc +1 more source
COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells
Molecular Oncology, EarlyView.This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos +6 more
wiley +1 more source
Molecular Cloning of Leukocyte Surface Molecules by Retrovirus-Mediated Expression Cloning System
Journal of Associated Medical Sciences, 2015 -
Sawitree Chiampanichayakul +1 more
doaj
MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE
Scientific Papers Animal Science and Biotechnologies, 2023 An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL +2 more
doaj
Two Distinct, Geographically Overlapping Lineages of the Corallimorpharian Ricordea Florida (Cnidaria: Hexacorallia: Ricordeidae) [PDF]
, 2011 We examined the genetic variation of the corallimorpharian Ricordea florida; it is distributed throughout the Caribbean region and is heavily harvested for the marine aquarium trade. Eighty-four distinct individuals of R. florida were sequenced from four
Lado-Insua, T. +4 more
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Molecular Oncology, EarlyView.Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang +12 more
wiley +1 more source
, 2017 Molecular cloning is a process that manipulates the spider silk sequence to select for a proper sequence size and specific vector into which it can be expressed. This process is achieved using techniques such as digests, transformations, purifications, and ligations.
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Medicago truncatula functional genomics: an invaluable resource for studies on agriculture sustainability [PDF]
, 2012 Legume functional genomics has moved many steps forward in the last two decades thanks to the improvement of genomics technologies and to the efforts of the research community.
Calderini, Ornella +2 more
core
Interrogating the immune landscape of microsatellite stable RAS‐mutated colon cancer
Molecular Oncology, EarlyView.COLOSSUS project RAS‐mutated MSS colon cancer study explored transcriptomics and immune cell density by immunohistochemistry (IHC), Immunoscore (IS), ISIC/TuLIS scores, mutation counts, and detected different prevalences but similar microenvironment composition across immune markers with clinical relevance for future immunotherapy combination ...
Rodrigo Dienstmann +61 more
wiley +1 more source