Molecular cloning and characterization of PLC‐η2 [PDF]
The FASEB Journal, 2005 Phospholipase C isozymes catalyze the conversion of phosphatidylinositol‐(4,5)P 2 to the Ca 2+ ‐mobilizing second messenger, Ins(1,4,5)P 3 , and the protein kinase C‐activating second messenger, diacylglycerol ...
Yixing, Zhou +3 more
openaire +2 more sources
Arguments for Human Therapeutic Cloning
Biomolecules & Biomedicine, 2008 For over 30 years, many Western governments have regulated scientific research involving human subjects. According to Knoppers implementation of regulation followed a long and checkered history of research abuse.
Krešimir Pavelić
doaj +1 more source
Molecular cloning of Anuroctoxin gene into pPICZαA: A yeast expression vector [PDF]
, 2021 Anuroctoxin gene was amplified from source plasmid and cloned into pPICZαA vector using the restriction ligation approach. The recombinant vector was transformed into bacterial strain E. coli using heat shock method.
Gembiczki, Júlia
core
Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research. [PDF]
PLoS ONE, 2017 Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-coding ...
Mostafa Aboulela +7 more
doaj +1 more source
cDNA Cloning Demonstrates the Expression of Pregnancy-Specific Glycoprotein Genes, a Subgroup of the Carcinoembryonic Antigen Gene Family, in Fetal Liver [PDF]
, 1989 The pregnancy-specific glycoprotein (PSG) genes constitute a subgroup of the carcinoembryonic antigen (CEA) gene family. Here we report the cloning of four cDNAs coding for different members of the PSG family from a human fetal liver cDNA library.
Zimmermann, Wolfgang +2 more
core +1 more source
The Role of “Adult‐Onset” Cancer Predisposition Genes in Pediatric Cancer: A Comprehensive Review
Pediatric Blood &Cancer, EarlyView.ABSTRACT Current literature estimates that 10% of pediatric cancers are caused by pathogenic or likely pathogenic (P/LP) germline variants in cancer predisposition genes (CPGs). Variants in CPGs thought to increase cancer risk exclusively during adulthood are referred to as “adult‐onset” CPGs (aoCPGs).
Maria Rozo +5 more
wiley +1 more source
Recombination-assisted megaprimer (RAM) cloning
MethodsX, 2014 No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive. Restriction-free cloning is among the simplest, most rapid, and cost-effective methods, but does not always provide ...
Jacques Mathieu +2 more
doaj +1 more source
In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR. [PDF]
PLoS ONE, 2017 The precise assembly of defined DNA sequences into plasmids is an essential task in bioscience research. While a number of molecular cloning techniques have been developed, many methods require specialized expensive reagents or laborious experimental ...
Faqing Huang +2 more
doaj +1 more source
Molecular cloning and physical mapping of murine cytomegalovirus DNA [PDF]
, 1983 Murine cytomegalovirus (MCMV) Smith strain DNA is cleaved by restriction endonuclease HindIII into 16 fragments, ranging in size from 0.64 to 22.25 megadaltons.
Ebeling, Angelika +3 more
core
FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source