BioTechniques, 2020 During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited.
Tomo Kondo, Shigehiko Yumura
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Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. [PDF]
PLoS ONE, 2013 The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites.
Stephan Kirchmaier +2 more
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Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls [PDF]
, 2007 Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid.
Klinghammer, Michaela, Tenhaken, Raimund
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Optimal cloning of PCR fragments by homologous recombination in Escherichia coli. [PDF]
PLoS ONE, 2015 PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown
Ana Paula Jacobus, Jeferson Gross
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An Experimental Framework to Examine the Influence of Promoter Architecture and Genomic Context on Gene Expression [PDF]
, 2020 Transcription is a fundamental process of gene expression. Information stored in DNA is transcribed into different types of mobile RNA, which play a role in various essential processes of the cell, e.g. translation.
Klein, Carlo Anton
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A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
BMC Biotechnology, 2010 Background The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences.
Nørholm Morten HH
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Metabolic Engineering Communications, 2016 Molecular Cloning Designer Simulator (MCDS) is a powerful new all-in-one cloning and genetic engineering design, simulation and management software platform developed for complex synthetic biology and metabolic engineering projects.
Zhenyu Shi, Claudia E. Vickers
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Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice
Czech Journal of Genetics and Plant Breeding, 2022 Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism,
Yu Li +6 more
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Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain [PDF]
, 2016 Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No.
Duan, Li-Peng +7 more
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High-throughput FastCloning technology: A low-cost method for parallel cloning
PLoS ONE, 2022 FastCloning, a reliable cloning technique for plasmid construction, is a widely used protocol in biomedical research laboratories. Only two-step molecular manipulations are required to add a gene (cDNA) of interest into the desired vector.
Hua Jiang +9 more
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