Results 21 to 30 of about 485,373 (308)
Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning
Frontiers in Bioengineering and Biotechnology, 2020 Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan +5 more
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, 2011 The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day.
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Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method [PDF]
PeerJ, 2018 Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min.
Lendl Tan +3 more
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Streamlined assembly of cloning and genome editing vectors for genus Clostridium
iScience, 2023 Summary: Reported herein is a new set of vectors designed to streamline molecular cloning and genome editing by exploiting modern cloning methods. The new vectors build on the existing pMTL8000 vectors that have been a staple of Clostridium research for ...
Tom S. Bailey +3 more
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Journal of Microbiology & Biology Education, 2023 Recombinant plasmids are essential tools in molecular biotechnology, and reliable plasmid assembly methods have, therefore, become a prerequisite for the successful cloning and transfer of genes.
Mihaela Bejenari +5 more
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HomeRun Vector Assembly System: a flexible and standardized cloning system for assembly of multi-modular DNA constructs. [PDF]
PLoS ONE, 2014 Advances in molecular and synthetic biology call for efficient assembly of multi-modular DNA constructs. We hereby present a novel modular cloning method that obviates the need for restriction endonucleases and significantly improves the efficiency in ...
Ming V Li +6 more
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Biomédica: revista del Instituto Nacional de Salud, 2008 Introducción. Los dos subgéneros en los cuales se divide el género Leishmania: Viannia y Leishmania, presentan diferencias significativas en las manifestaciones clínicas que causan, en su comportamiento de crecimiento en cultivos in vitro, en sus ...
Marcel Marín +4 more
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BioTechniques, 2023 With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran +4 more
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Molecular cloning of a bovine cathepsin [PDF]
Biochemical Journal, 1985 A cDNA clone for a thiol endoproteinase has been isolated from a bovine heart cDNA library by using a mixture of 32 synthetic oligonucleotides as a hybridization probe. The inserted region is 672 base pairs in length. It contains a sequence encoding the C-terminal region of a protein that is homologous to rat liver cathepsins B and H and to plant thiol
N J, Gay, J E, Walker
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Primers for molecular cloning.
, 2021 Primers for molecular cloning.
Herve Migaud (308426) +5 more
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