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Biomédica: revista del Instituto Nacional de Salud, 2008 Introducción. Los dos subgéneros en los cuales se divide el género Leishmania: Viannia y Leishmania, presentan diferencias significativas en las manifestaciones clínicas que causan, en su comportamiento de crecimiento en cultivos in vitro, en sus ...
Marcel Marín +4 more
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BioTechniques, 2023 With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran +4 more
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Molecular cloning of a bovine cathepsin [PDF]
Biochemical Journal, 1985 A cDNA clone for a thiol endoproteinase has been isolated from a bovine heart cDNA library by using a mixture of 32 synthetic oligonucleotides as a hybridization probe. The inserted region is 672 base pairs in length. It contains a sequence encoding the C-terminal region of a protein that is homologous to rat liver cathepsins B and H and to plant thiol
N J, Gay, J E, Walker
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Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning [PDF]
, 2010 Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Neil G. Stoker +15 more
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iScience, 2019 Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning.
Bryan Bishé +2 more
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Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis [PDF]
, 1986 Friehs K, Schörgendorfer K, Schwab H, Lafferty RM. Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology.
Lafferty, R. M. +3 more
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BioTechniques, 2019 Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Jens Staal +4 more
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HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review
Biomedicines, 2021 Oxygen is essential for the maintenance of the body. Living organisms have evolved systems to secure an oxygen environment to be proper. Hypoxia-inducible factor (HIF) plays an essential role in this process; it is a transcription factor that mediates ...
Kiichi Hirota
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BioTechniques, 2020 During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited.
Tomo Kondo, Shigehiko Yumura
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Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. [PDF]
PLoS ONE, 2013 The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites.
Stephan Kirchmaier +2 more
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