Results 21 to 30 of about 3,746,077 (303)

HomeRun Vector Assembly System: a flexible and standardized cloning system for assembly of multi-modular DNA constructs. [PDF]

PLoS ONE, 2014
Advances in molecular and synthetic biology call for efficient assembly of multi-modular DNA constructs. We hereby present a novel modular cloning method that obviates the need for restriction endonucleases and significantly improves the efficiency in ...
Ming V Li, Dip Shukla, Brian H Rhodes, Anjali Lall, Jingmin Shu, Branden S Moriarity, David A Largaespada   +6 more
doaj   +1 more source

El análisis molecular y el inmunogénico sugieren la ausencia de las proteínas hidrofílicas de superficie en Leishmania (Viannia) panamensis

Biomédica: revista del Instituto Nacional de Salud, 2008
Introducción. Los dos subgéneros en los cuales se divide el género Leishmania: Viannia y Leishmania, presentan diferencias significativas en las manifestaciones clínicas que causan, en su comportamiento de crecimiento en cultivos in vitro, en sus ...
Marcel Marín, Yudy Alexandra Aguilar, José Robinson Ramírez, Omar Triana, Carlos Enrique Muskus   +4 more
doaj   +1 more source

Molecular cloning of a bovine cathepsin [PDF]

Biochemical Journal, 1985
A cDNA clone for a thiol endoproteinase has been isolated from a bovine heart cDNA library by using a mixture of 32 synthetic oligonucleotides as a hybridization probe. The inserted region is 672 base pairs in length. It contains a sequence encoding the C-terminal region of a protein that is homologous to rat liver cathepsins B and H and to plant thiol
N J, Gay, J E, Walker
openaire   +2 more sources

PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs

BioTechniques, 2023
With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran, Ryan D Hallam, Marvel Megaly, Anel Turgambayeva, Aleksandar Necakov   +4 more
doaj   +1 more source

The Cloning of the Human Tumor Supressor Gene INGI: DNA Cloning into Plasmid Vector and DNA Analysis by Restriction Enzymes

Journal of Dentistry Indonesia, 2015
DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari, Mamoru Ouchida, Mehmet Gunduz   +2 more
doaj   +1 more source

Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting

iScience, 2019
Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning.
Bryan Bishé, Arnaud Taton, James W. Golden   +2 more
doaj   +1 more source

Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site

BioTechniques, 2019
Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Jens Staal, Kübra Alci, Wouter De Schamphelaire, Martine Vanhoucke, Rudi Beyaert   +4 more
doaj   +1 more source

HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review

Biomedicines, 2021
Oxygen is essential for the maintenance of the body. Living organisms have evolved systems to secure an oxygen environment to be proper. Hypoxia-inducible factor (HIF) plays an essential role in this process; it is a transcription factor that mediates ...
Kiichi Hirota
doaj   +1 more source

Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning [PDF]

, 2010
Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Neil G. Stoker, Stoker, N G, Francois-Xavier Chauviac, Balhana, R J C, Sikder, M H, Stoker, Neil G., Chauviac, Francois-Xavier, Sharon L. Kendall, Sikder, Mahmudul Hasan, Kendall, S L, Birkbeck College, Chauviac, F-X, Mahmudul Hasan Sikder, Kendall, Sharon L., Ricardo Balhana, Balhana, Ricardo   +15 more
core   +1 more source

An improved molecular tool for screening bacterial colonies using GFP expression enhanced by a Dictyostelium sequence

BioTechniques, 2020
During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited.
Tomo Kondo, Shigehiko Yumura
doaj   +1 more source