Results 41 to 50 of about 2,933,423 (207)

DNA looping in gene regulation: from the assembly of macromolecular complexes to the control of transcriptional noise [PDF]

Current Opinion in Genetics & Development, 15, 136-144 (2005), 2005
The formation of DNA loops by proteins and protein complexes that bind at distal DNA sites plays a central role in many cellular processes, such as transcription, recombination, and replication. Here we review the basic thermodynamic concepts underlying the assembly of macromolecular complexes on looped DNA and the effects that this process has in the ...
arxiv   +1 more source

Recombinant pharmaceuticals from microbial cells: a 2015 update

Microbial Cell Factories, 2016
Diabetes, growth or clotting disorders are among the spectrum of human diseases related to protein absence or malfunction. Since these pathologies cannot be yet regularly treated by gene therapy, the administration of functional proteins produced ex vivo
L. Sánchez‐García, Lucas Martín, R. Mangues, N. Ferrer-Miralles, E. Vázquez, A. Villaverde   +5 more
semanticscholar   +1 more source

Physics of RecA-mediated homologous recognition [PDF]

, 2004
Most proteins involved in processing DNA accomplish their activities as a monomer or as a component of a multimer containing a relatively small number of other elements. They generally act locally, binding to one or a few small regions of the DNA substrate. Striking exceptions are the \textit{E.
arxiv   +1 more source

Removing N-terminal sequences in pre-S1 domain enhanced antibody and B-cell responses by an HBV large surface antigen DNA vaccine. [PDF]

PLoS ONE, 2012
Although the use of recombinant hepatitis B virus surface (HBsAg) protein vaccine has successfully reduced global hepatitis B infection, there are still a number of vaccine recipients who do not develop detectable antibody responses.
Guohong Ge, Shixia Wang, Yaping Han, Chunhua Zhang, Shan Lu, Zuhu Huang   +5 more
doaj   +1 more source

Rescue of influenza A virus from recombinant DNA.

Journal of Virology, 2007
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme ...
E. Fodor, E. Fodor, L. Devenish, O. Engelhardt, P. Palese, G. Brownlee, A. García-Sastre   +6 more
semanticscholar   +1 more source

Newcastle Disease Virus Expressing Cap Gene of Porcine Circovirus Type 2 Confers Protection in Mice and Induced Long-Lasting Neutralizing Antibodies in Pigs

Vaccines
Background/Objectives: Porcine Circovirus 2 (PCV2) infection poses significant health and economic challenges to the global swine industry. The disease in pigs leads to lymphoid depletion, resulting in immunosuppression and increased susceptibility to co-
Sohini Dey, Rudhreswaran Murugasamy, Lukumoni Buragohain, Ajai Lawrence D’silva, Jayashree Sarma, Arpita Bharali, Saravanan Ramakrishnan, Mani Saminathan, Nagendra Nath Barman, Vikram N. Vakharia, Madhan Mohan Chellappa   +10 more
doaj   +1 more source

High fidelity of RecA-catalyzed recombination: a watchdog of genetic diversity [PDF]

Nucleic Acids Research, 2006, Vol. 34, No. 18 5021-5031, 2010
Homologous recombination plays a key role in generating genetic diversity, while maintaining protein functionality. The mechanisms by which RecA enables a single-stranded segment of DNA to recognize a homologous tract within a whole genome are poorly understood.
arxiv   +1 more source

A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.

Proceedings of the National Academy of Sciences of the United States of America, 1988
A highly conserved repetitive DNA sequence, (TTAGGG)n, has been isolated from a human recombinant repetitive DNA library. Quantitative hybridization to chromosomes sorted by flow cytometry indicates that comparable amounts of this sequence are present on
R. Moyzis, J. Buckingham, L. Cram, M. Dani, L. Deaven, M. Jones, J. Meyne, R. Ratliff, Jung-Rung Wu   +8 more
semanticscholar   +1 more source

Insights into Alkaline Phosphatase Anti-Inflammatory Mechanisms

Biomedicines
Background: The endogenous ecto-enzyme and exogenously administered alkaline phosphatase (ALP) have been evidenced to significantly attenuate inflammatory conditions, including Toll-like receptor 4 (TLR4)-related signaling and cytokine overexpression ...
Larissa Balabanova, Georgii Bondarev, Aleksandra Seitkalieva, Oksana Son, Liudmila Tekutyeva   +4 more
doaj   +1 more source

Direct homologous dsDNA-dsDNA pairing: how, where and why? [PDF]

J. Mol. Biol. 290, 373-377, 2020, 2019
The ability of homologous chromosomes (or selected chromosomal loci) to pair specifically in the apparent absence of DNA breakage and recombination represents a prominent feature of eukaryotic biology. The mechanism of homology recognition at the basis of such recombination-independent pairing has remained elusive.
arxiv   +1 more source