Results 41 to 50 of about 3,265,618 (328)

CRISPR/Cpf1-mediated DNA-free plant genome editing

Nature Communications, 2017
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing
Hyeran Kim, Sang-Tae Kim, Jahee Ryu, Beum-Chang Kang, Jin-Soo Kim, Sang-Gyu Kim   +5 more
semanticscholar   +1 more source

Specific lid-base contacts in the 26s proteasome control the conformational switching required for substrate degradation. [PDF]

, 2019
The 26S proteasome is essential for proteostasis and the regulation of vital processes through ATP-dependent degradation of ubiquitinated substrates. To accomplish the multi-step degradation process, the proteasomes regulatory particle, consisting of lid
Aufderheide, Bard, Bard, Bard, Bashore, Beckwith, Dambacher, de la Peña, Ding, Dong, Eisele, Elsasser, Finley, Glickman, Glickman, Goldstein, Groll, Hershko, Kachroo, Lander, Lasker, Longtine, Matyskiela, Myers, Nemec, Peth, Prakash, Rubin, Smith, Sone, Tomko, Unverdorben, Verma, VerPlank, VerPlank, Wehmer, Wendler, Wenzel, Worden, Worden, Yao, Zhu, Śledź   +42 more
core   +1 more source

Phase 1 safety and immunogenicity testing of DNA and recombinant modified vaccinia Ankara vaccines expressing HIV-1 virus-like particles.

Journal of Infectious Diseases, 2011
BACKGROUND Recombinant DNA and modified vaccinia virus Ankara (rMVA) vaccines represent a promising approach to an HIV/AIDS vaccine. This Phase 1 clinical trial compared the safety and immunogenicity of a rMVA vaccine administered with and without DNA ...
P. Goepfert, M. Elizaga, A. Sato, L. Qin, M. Cardinali, C. Hay, J. Hural, S. DeRosa, O. Defawe, G. Tomaras, D. Montefiori, Yongxian Xu, L. Lai, S. Kalams, L. Baden, S. Frey, W. Blattner, L. Wyatt, B. Moss, H. Robinson   +19 more
semanticscholar   +1 more source

Evaluation of virulence genes in Proteus strains isolated from diabetic foot infections and urinary tract infections

Journal of Infection in Developing Countries
Introduction: Proteus species are frequently isolated from urinary tract infections (UTIs) and diabetic foot infections (DFIs). We aimed to evaluate the presence of virulence genes in P. mirabilis and P.
Ruth M Phiri, Mustafa Bülent Ertuğrul, Bülent Bozdoğan, Tuğrul Hoşbul   +3 more
doaj   +1 more source

Construction and Immunogenicity Testing of Salmonella, STM1 Vaccine Vector Expressing HIV-1 Antigen

Indonesian Journal of Biotechnology, 2009
Objective of this study: to determine the ability of Salmonella enterica serovar Typhimurium STM1 as a delivery vehicle for the HIV p24 gene and HIV env gene.
Endang Winiati Bachtiar, Peter Smooker, Peter J Coloe   +2 more
doaj   +1 more source

APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks [PDF]

, 2007
Aprataxin and polynucleotide kinase (PNK) are DNA end processing factors that are recruited into the DNA single- and double-strand break repair machinery through phosphorylation-specific interactions with XRCC1 and XRCC4, respectively. These interactions
Caldecott, Keith W, El-Khamisy, Sherif F, Iles, Natasha, Rulten, Stuart   +3 more
core   +5 more sources

QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations.

The Plant Journal, 2013
The majority of agronomically important crop traits are quantitative, meaning that they are controlled by multiple genes each with a small effect (quantitative trait loci, QTLs).
H. Takagi, A. Abe, Kentaroh Yoshida, S. Kosugi, S. Natsume, Chikako Mitsuoka, Aiko Uemura, Hiroe Utsushi, Muluneh Tamiru, Shohei Takuno, H. Innan, L. Cano, S. Kamoun, R. Terauchi   +13 more
semanticscholar   +1 more source

Population, sexual and reproductive health, rights and sustainable development: forging a common agenda. [PDF]

, 2010
This article suggests that sexual and reproductive health and rights activists seeking to influence the post-2015 international development paradigm must work with sustainable development advocates concerned with a range of issues, including climate ...
Campbell, D. M., Carter, P. E., Collins-Emerson, J. M., French, N. P., Hathaway, S., Marshall, J., Midwinter, A. C., Mullner, P., Pirie, R., Shadbolt, T., Spencer, Simon E. F., Wilson, D. J.   +11 more
core   +1 more source

Cloning and expression of the Propionibacterium shermanii methylmalonyl-CoA epimerase gene in Escherichia coli : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]

, 1994
Genomic DNA was isolated from Propionibacterium shermanii (52W). A 454 bp DNA fragment coding for the methylmalonyl-CoA epimerase (EC 5.1.99.1, subsequently referred to as epimerase) was amplified from genomic DNA by the polymerase chain reaction using ...
Saafi, 'Etuate Lui
core  

Characterization of small molecules inhibiting the pro-angiogenic activity of the zinc finger transcription factor Vezf1 [PDF]

, 2018
Discovery of inhibitors for endothelial-related transcription factors can contribute to the development of anti-angiogenic therapies that treat various diseases, including cancer.
Gowher, Humaira, He, Ming, Norvil, Allison B, Sherris, David, Yang, Qianyi   +4 more
core   +2 more sources