Results 51 to 60 of about 894,226 (301)

Expression and purification of recombinant human coagulation Factor VII fused to His-Tag through Gateway technology [PDF]

Background. Factor VII is a plasma glycoprotein that participates in the coagulation process leading to generation of fibrin. Construction, expression and purification of recombinant FVII fused to poly histidin tag through gateway technology ...
حلبیان, راحله, عدالتی فتح آباد, مهدی, مسروری, ناصر, محمدی روشنده, آمنه, ساکی, ساسان, امیری زاده, ناصر, حبیبی رودکنار, مهریار   +6 more
core   +1 more source

Cytolytic T lymphocyte recognition of the murine cytomegalovirus nonstructural immediate-early protein pp89 expressed by recombinant vaccinia virus [PDF]

, 1987
The murine immediate-early (IE) protein pp89 is a nonstructural virus- encoded phosphoprotein residing in the nucleus of infected cells, where it acts as transcriptional activator.
Bertholet, Christine, Jonjic, Stipan, Koszinowski, Ulrich H., Volkmer, Hansjürgen, Wittek, Riccardo   +4 more
core   +4 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate

Indonesian Journal of Biotechnology, 2005
Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during active penetration of parasite into the cell target.
Wayan T. Artama, Yulia Sari, Didik Tulus Subekti, Soenarwan Hery Poerwanto, Jarot Subandono   +4 more
doaj   +1 more source

Impact of PNKP mutations associated with microcephaly, seizures and developmental delay on enzyme activity and DNA strand break repair [PDF]

, 2012
Microcephaly with early-onset, intractable seizures and developmental delay (MCSZ) is a hereditary disease caused by mutations in polynucleotide kinase/phosphatase (PNKP), a DNA strand break repair protein with DNA 5'-kinase and DNA 3'-phosphatase ...
Alexandra K. Walker, Beckman, Bernstein, Breslin, Breslin, Brodsky, Buck, Buck, Caldecott, Caldecott, Chappell, Christopher A. Walsh, Date, Dingwall, Dobson, Edward C. Gilmore, El-Khamisy, Evans, Garces, Hsiang, Interthal, John J. Reynolds, Karimi-Busheri, Keith W. Caldecott, Koch, Kouzminova, Kuzminov, Lee, Lindahl, Loizou, McCullough, Moreira, Riballo, Segal-Raz, Shen, Sprules, Takashima, Wang, Wang, Ward, Ward, Whitehouse   +41 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Mutant and chimeric recobinant plasminogen activatorsproduction in eukaryotic cellsand preliminary characterization [PDF]

, 1987
Mutant urokinase-type plasminogen activator (u-PA) genes and hybrid genes between tissue-type plasminogen activator (t-PA) and u-PA have been designed to direct the synthesis of new plasminogen activators and to investigate the structure-function ...
Andre, B., Bollen, A., Collen, D., Cravador, A., Deforesta, F., Dewilde, M., Gheysen, D., Herzog, A., Hoylaerts, M., Jacobs, P., Pierard, L., Topisirovic, L.   +11 more
core   +1 more source

Characterization of Knots and Links Arising From Site-specific Recombination on Twist Knots

, 2011
We develop a model characterizing all possible knots and links arising from recombination starting with a twist knot substrate, extending previous work of Buck and Flapan. We show that all knot or link products fall into three well-understood families of
Buck D, Burde G, Conway J H, Cox M M, Cozzarelli N R, Craig N, Cromwell P, Darcy I K Ishihara K Medikonduri R Shimokawa K, Dorothy Buck, Karin Valencia, Kawauchi A, Maxwell A, Rolfsen D, Shimokawa K Ishihara K Vazquez M, Valencia K Buck D, Wang James C, Zechiedrich L E   +16 more
core   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Comparison of Plasmid yield between L.B. and using Molasses as a medium [PDF]

, 2009
During the initial studies I have found that the molasses may be a good alternative to costly L.B media where one can manipulate the C/N ratio as per requirement of cell.
Nitin Sharma, Purshottum Kaushik
core   +2 more sources