Results 61 to 70 of about 3,265,618 (328)
Screening for the optimal siRNA targeting a novel gene (HA117) and construction and evaluation of a derivative recombinant adenovirus [PDF]
, 2010 We found a novel gene named as HA117 in our previous research. At this study, we screened for an optimal siRNA targeting the novel gene HA117 using the pSOS-HUS method, verified the results of pSOS-HUS siRNA screening for optimal affinity for the target ...
Gaihuan Zheng +4 more
core +1 more source
Cloning and expression of codon-optimized recombinant darbepoetin alfa in Leishmania tarentolae T7-TR [PDF]
, 2016 Darbepoetin alfa is an engineered and hyperglycosylated analog of recombinant human erythropoietin (EPO) which is used as a drug in treating anemia in patients with chronic kidney failure and cancer.
Fard-Esfahani, P. +8 more
core +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 1988 A highly conserved repetitive DNA sequence, (TTAGGG)n, has been isolated from a human recombinant repetitive DNA library. Quantitative hybridization to chromosomes sorted by flow cytometry indicates that comparable amounts of this sequence are present on
R. Moyzis +8 more
semanticscholar +1 more source
FEBS Letters, EarlyView.C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi +5 more
wiley +1 more source
Pesquisa Veterinária Brasileira, 2013 The ability of thymidine kinase (tk)-deleted recombinant bovine herpesvirus 5 (BoHV-5tkΔ) to establish and reactivate latent infection was investigated in lambs. During acute infection, the recombinant virus replicated moderately in the nasal mucosa, yet
Gustavo C. Cadore +5 more
doaj +1 more source
Recombinant subunit vaccines against Neospora caninum [PDF]
, 2007 The intracellular protozoan parasite Neospora caninum is an important cause of bovine abortion and congenital infection in many countries. Currently there is no effective control method available, and development of a vaccine has been suggested as a ...
Pinitkiatisakul, Sunan
core
A multi-landing pad DNA integration platform for mammalian cell engineering
Nucleic Acids Research, 2018 Engineering mammalian cell lines that stably express many transgenes requires the precise insertion of large amounts of heterologous DNA into well-characterized genomic loci, but current methods are limited. To facilitate reliable large-scale engineering
L. Gaidukov +16 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu +11 more
wiley +1 more source
Communications Biology, 2022 DNA methyltransferase 3A (DNMT3A) is shown to efficiently methylate recombinant mononucleosome linker DNA and a role for H3K36me3 in the stimulation of DNA methylation is revealed that is independent of its interaction with the PWWP domain.
Alexander Bröhm +8 more
doaj +1 more source
, 2013 Recombinant DNA is the formation of a novel DNA sequence by the combination of two DNA fragments. It involves the method by which DNA of the donor organism (target DNA) is cut into fragments with the help of Restriction Enzymes and insert one of these fragments into the DNA of the host.
openaire +2 more sources