Results 61 to 70 of about 2,933,423 (207)
The aim of this study was to develop homogeneous and stable plasmid DNA reference materials for detecting the mechanisms of resistance to quinolones and fluoroquinolones in foodborne pathogens.
Qinya Niu+10 more
doaj +1 more source
RecA-mediated homology search as a nearly optimal signal detection system [PDF]
Homologous recombination facilitates the exchange of genetic material between homologous DNA molecules. This crucial process requires detecting a specific homologous DNA sequence within a huge variety of heterologous sequences. The detection is mediated by RecA in E. coli, or members of its superfamily in other organisms.
arxiv +1 more source
Generation of recombinant influenza virus from plasmid DNA.
Efforts by a number of influenza research groups have been pivotal in the development and improvement of influenza A virus reverse genetics. Originally established in 1999 plasmid-based reverse genetic techniques to generate recombinant viruses have ...
L. Martínez-Sobrido, A. García-Sastre
semanticscholar +1 more source
Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate
Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during active penetration of parasite into the cell target.
M. Murwantoko+2 more
doaj +1 more source
Genomic presence of recombinant porcine endogenous retrovirus in transmitting miniature swine
The replication of porcine endogenous retrovirus (PERV) in human cell lines suggests a potential infectious risk in xenotransplantation. PERV isolated from human cells following cocultivation with porcine peripheral blood mononuclear cells is a ...
Wilkinson Robert+2 more
doaj +1 more source
Assessing optimal: inequalities in codon optimization algorithms
Background Custom genes have become a common resource in recombinant biology over the last 20 years due to the plummeting cost of DNA synthesis. These genes are often “optimized” to non-native sequences for overexpression in a non-native host by ...
Matthew J. Ranaghan+3 more
doaj +1 more source
Recombinant transfer in the basic genome of E. coli [PDF]
An approximation to the ~4 Mbp basic genome shared by 32 strains of E. coli representing six evolutionary groups has been derived and analyzed computationally. A multiple-alignment of the 32 complete genome sequences was filtered to remove mobile elements and identify the most reliable ~90% of the aligned length of each of the resulting 496 basic ...
arxiv +1 more source
Bacillus thuringiensis (Bt) subsp. medellin (Btmed) produces parasporal crystalline inclusions which are toxic to mosquito larvae. It has been shown that the inclusions of this bacterium contain mainly proteins of 94, 68 and 28-30 kDa.
Nora Restrepo+5 more
doaj +1 more source
Statistical analysis on detecting recombination sites in DNA-beta satellites associated with the old world geminiviruses [PDF]
Although an exchange of genetic information by recombination plays an important role in the evolution of viruses, it is not clear how it generates diversity. {\it Geminiviruses} are plant viruses which have ambisense single-stranded circular DNA genomes and one of the most economically important plant viruses in agricultural production.
arxiv
DNA coding for bacteriophage T7 RNA polymerase was ligated to a vaccinia virus transcriptional promoter and integrated within the vaccinia virus genome.
T. Fuerst+3 more
semanticscholar +1 more source