Results 61 to 70 of about 2,933,423 (207)

Developing Qualitative Plasmid DNA Reference Materials to Detect Mechanisms of Quinolone and Fluoroquinolone Resistance in Foodborne Pathogens

open access: yesFoods, 2022
The aim of this study was to develop homogeneous and stable plasmid DNA reference materials for detecting the mechanisms of resistance to quinolones and fluoroquinolones in foodborne pathogens.
Qinya Niu   +10 more
doaj   +1 more source

RecA-mediated homology search as a nearly optimal signal detection system [PDF]

open access: yesMolecular Cell 40(3) 388-396 (2010), 2010
Homologous recombination facilitates the exchange of genetic material between homologous DNA molecules. This crucial process requires detecting a specific homologous DNA sequence within a huge variety of heterologous sequences. The detection is mediated by RecA in E. coli, or members of its superfamily in other organisms.
arxiv   +1 more source

Generation of recombinant influenza virus from plasmid DNA.

open access: yesJournal of Visualized Experiments, 2010
Efforts by a number of influenza research groups have been pivotal in the development and improvement of influenza A virus reverse genetics. Originally established in 1999 plasmid-based reverse genetic techniques to generate recombinant viruses have ...
L. Martínez-Sobrido, A. García-Sastre
semanticscholar   +1 more source

Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate

open access: yesIndonesian Journal of Biotechnology, 2008
Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during active penetration of parasite into the cell target.
M. Murwantoko   +2 more
doaj   +1 more source

Genomic presence of recombinant porcine endogenous retrovirus in transmitting miniature swine

open access: yesVirology Journal, 2006
The replication of porcine endogenous retrovirus (PERV) in human cell lines suggests a potential infectious risk in xenotransplantation. PERV isolated from human cells following cocultivation with porcine peripheral blood mononuclear cells is a ...
Wilkinson Robert   +2 more
doaj   +1 more source

Assessing optimal: inequalities in codon optimization algorithms

open access: yesBMC Biology, 2021
Background Custom genes have become a common resource in recombinant biology over the last 20 years due to the plummeting cost of DNA synthesis. These genes are often “optimized” to non-native sequences for overexpression in a non-native host by ...
Matthew J. Ranaghan   +3 more
doaj   +1 more source

Recombinant transfer in the basic genome of E. coli [PDF]

open access: yes, 2015
An approximation to the ~4 Mbp basic genome shared by 32 strains of E. coli representing six evolutionary groups has been derived and analyzed computationally. A multiple-alignment of the 32 complete genome sequences was filtered to remove mobile elements and identify the most reliable ~90% of the aligned length of each of the resulting 496 basic ...
arxiv   +1 more source

Cloning, Expression and Toxicity of a Mosquitocidal Toxin Gene of Bacillus thuringiensis subsp. medellin

open access: yesMemorias do Instituto Oswaldo Cruz, 1997
Bacillus thuringiensis (Bt) subsp. medellin (Btmed) produces parasporal crystalline inclusions which are toxic to mosquito larvae. It has been shown that the inclusions of this bacterium contain mainly proteins of 94, 68 and 28-30 kDa.
Nora Restrepo   +5 more
doaj   +1 more source

Statistical analysis on detecting recombination sites in DNA-beta satellites associated with the old world geminiviruses [PDF]

open access: yesarXiv, 2010
Although an exchange of genetic information by recombination plays an important role in the evolution of viruses, it is not clear how it generates diversity. {\it Geminiviruses} are plant viruses which have ambisense single-stranded circular DNA genomes and one of the most economically important plant viruses in agricultural production.
arxiv  

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1986
DNA coding for bacteriophage T7 RNA polymerase was ligated to a vaccinia virus transcriptional promoter and integrated within the vaccinia virus genome.
T. Fuerst   +3 more
semanticscholar   +1 more source

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