Results 21 to 30 of about 1,065,519 (331)

Epidemiological Typing of Moraxella Catarrhalis by Pulsed Field Gel Electrophoresis

Canadian Journal of Infectious Diseases, 1995
Pulsed field gel electrophoresis (pfge) was used to compare 59 strains of Moraxella catarrhalis to evaluate pfge for the epidemiological typing of this organism.
Susan M Davison, Donald E Low, Rene H Cruz, Danielle Beaulieu, Shelley R Scriver   +4 more
doaj   +1 more source

Hybridization Parameters Revisited: Solutions Containing SDS

BioTechniques, 2002
Salt concentration governs nucleic acid hybridization according to the Schildkraut-Lifson equation. High concentrations of SDS are used in some common protocols, but the effects of SDS on hybridization stringency have not been reported.
Ken Rose, John O. Mason, Richard Lathe
doaj   +1 more source

Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA [PDF]

, 1977
The rate of reaction of excess double-stranded bacteriophage phi X174 and plasmid RSF2124 DNA drivers with enzymatically synthesized asymmetric RNA tracers was measured. Other reactions were carried out with excess Escherichia coli DNA and E.
Britten, Roy J., Davidson, Eric H., Galau, Glenn A., Smith, Michael J.   +3 more
core   +1 more source

Taxonomic heterogeneity within the Planctomycetales as derived by DNA-DNA hybridization, description of Rhodopirellula baltica gen. nov., sp. nov., transfer of Pirellula marina to the genus Blastopirellula gen. nov. as Blastopirellula marina comb. nov. and emended description of the genus Pirellula.

International Journal of Systematic and Evolutionary Microbiology, 2004
Ninety-seven strains of budding bacteria originating from various aquatic habitats and morphologically resembling planctomycetes were investigated taxonomically.
H. Schlesner, Christina Rensmann, B. Tindall, Dörte Gade, R. Rabus, S. Pfeiffer, P. Hirsch   +6 more
semanticscholar   +1 more source

Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges

BMC Biotechnology, 2008
Background The high binding specificity of short 10 to 30 mer oligonucleotide probes enables single base mismatch (MM) discrimination and thus provides the basis for genotyping and resequencing microarray applications.
Mai Timo, Kayser Jona, Ehler Oliver, Naiser Thomas, Michel Wolfgang, Ott Albrecht   +5 more
doaj   +1 more source

On Critical Relative Distance of DNA Codes for Additive Stem Similarity [PDF]

, 2010
We consider DNA codes based on the nearest-neighbor (stem) similarity model which adequately reflects the "hybridization potential" of two DNA sequences.
D'yachkov, A., Macula, A., Renz, T., Rykov, V., Voronina, A.   +4 more
core   +3 more sources

Complete rpoB gene sequencing as a suitable supplement to DNA-DNA hybridization for bacterial species and genus delineation.

International Journal of Systematic and Evolutionary Microbiology, 2008
DNA-DNA hybridization (DDH), the gold standard for bacterial species delineation, is a laborious method and the alternative, average nucleotide identity (ANI), a genomic sequence-derived parameter, is not applicable to non-sequenced species.
T. Adékambi, T. Shinnick, D. Raoult, M. Drancourt   +3 more
semanticscholar   +1 more source

Design and analysis of linear cascade DNA hybridization chain reactions using DNA hairpins

New Journal of Physics, 2017
DNA self-assembly has been employed non-conventionally to construct nanoscale structures and dynamic nanoscale machines. The technique of hybridization chain reactions by triggered self-assembly has been shown to form various interesting nanoscale ...
Hieu Bui, Sudhanshu Garg, Vincent Miao, Tianqi Song, Reem Mokhtar, John Reif   +5 more
doaj   +1 more source

Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe [PDF]

, 1992
A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures.
Eichhorn, Werner, Hübert, P. H., Meyer, H., Schwend, C.   +3 more
core   +1 more source

DNA Sensing Platforms: Novel Insights into Molecular Grafting Using Low Perturbative AFM Imaging

Sensors, 2023
By using AFM as a nanografting tool, we grafted micrometer-sized DNA platforms into inert alkanethiol SAMs. Tuning the grafting conditions (surface density of grafting lines and scan rate) allowed us to tailor the molecular density of the DNA platforms ...
Silvia Maria Cristina Rotondi, Paolo Canepa, Elena Angeli, Maurizio Canepa, Ornella Cavalleri   +4 more
doaj   +1 more source