Stacking-induced fluorescence increase reveals allosteric interactions through DNA [PDF]
From gene expression to nanotechnology, understanding and controlling DNA requires a detailed knowledge of its higher order structure and dynamics. Here we take advantage of the environment-sensitive photoisomerization of cyanine dyes to probe local and ...
Lopez, Sergio G. +4 more
core +1 more source
Phase Transition of DNA-Linked Gold Nanoparticle
Melting and hybridization of DNA-capped gold nanoparticle networks are investigated with optical absorption spectroscopy. Single-stranded, 12-base DNA-capped gold nanoparticles are linked with complementary, single-stranded, 24-base linker DNA to form ...
Ching-Hwa Kiang +14 more
core +1 more source
The effects of mismatches on hybridization in DNA microarrays: determination of nearest neighbor parameters [PDF]
Quantifying interactions in DNA microarrays is of central importance for a better understanding of their functioning. Hybridization thermodynamics for nucleic acid strands in aqueous solution can be described by the so-called nearest-neighbor model ...
Bloomfield +23 more
core +3 more sources
Kindred DNA Amplification from Two Distinct Populations of cDNA Fragments
Kindred DNA amplification is a novel and cost-effective method developed to isolate common cDNA fragments between two distinct cDNA populations. Unlike subtractive hybridization, which discards common sequences, kindred DNA amplification isolates and ...
Ulo Puurand, Lumme Kadaja, Enn K. Seppet
doaj +1 more source
This paper aimed to understand and compare the two popular cytogenetic techniques of fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in detecting breast cancer chromosomal abnormality.
Harem Othman Smail
doaj +1 more source
Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. [PDF]
An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode.
Abu Bakar, Fatimah +4 more
core +1 more source
Reusing Nylon Membranes for Radioactive Hybridizations
We describe a procedure for recycling nylon hybridization membranes, enabling their repeated use for radioactive Southern hybridization analysis of different DNA samples.
David W. Thornbury, Mark L. Farman
doaj +1 more source
Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization [PDF]
A new strategy for analyzing chromosomal evolution in primates is presented using chromosomal in situ suppression (CISS) hybridization. Biotin-labeled DNA libraries from flow-sorted human chromosomes are hybridized to chromosome preparations of ...
Anna Jauch +22 more
core +1 more source
Impedance Spectroscopy Technique for DNA Hybridization
The development of biosensors for detection and identification of DNA sequences by hybridization may reduce assay time and allow direct quantitation of the target.
A. Tlili, A. Abdelghani, S. Hleli
doaj +1 more source
Sequence organization of feline leukemis virus DNA in infected cells [PDF]
A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W. +3 more
core +1 more source

