Results 51 to 60 of about 1,065,519 (331)

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

High-Sensitivity Hybridization Assay for Quantitation of Residual E. coli DNA

BioTechniques, 2002
Impurity assays for recombinant protein therapeutics are essential to ensure batchto-batch consistency and to meet the FDA's criteria for a well-characterized biopharmaceutical.
Xiaoying Ji, Kwan Lee, Byron DiPaolo
doaj   +1 more source

Defects Can Increase the Melting Temperature of DNA-Nanoparticle Assemblies

, 2007
DNA-gold nanoparticle assemblies have shown promise as an alternative technology to DNA microarrays for DNA detection and RNA profiling. Understanding the effect of DNA sequences on the melting temperature of the system is central to developing reliable ...
Harris, Nolan C., Kiang, Ching-Hwa
core   +1 more source

Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries [PDF]

, 1992
We established chromosomal homologies between all chromosomes of the human karyotype and that of an old world monkey (Macaca fuscata) by chromosomal in situ suppression (CISS) hybridization with human chromosome specific DNA libraries.
Cremer, Thomas, Jauch, Anna, Stanyon, Roscoe, Wienberg, Johannes   +3 more
core   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

An analysis of the use of genomic DNA as a universal reference in two channel DNA microarrays

BMC Genomics, 2005
Background DNA microarray is an invaluable tool for gene expression explorations. In the two-dye microarray, fluorescence intensities of two samples, each labeled with a different dye, are compared after hybridization.
Kapur Vivek, Gadgil Chetan, Lian Wei, Gadgil Mugdha, Hu Wei-Shou   +4 more
doaj   +1 more source

Remote Toehold: A Mechanism for Flexible Control of DNA Hybridization Kinetics [PDF]

, 2011
Hybridization of DNA strands can be used to build molecular devices, and control of the kinetics of DNA hybridization is a crucial element in the design and construction of functional and autonomous devices. Toehold-mediated strand displacement
Bath, Jonathan, Genot, Anthony J., Turberfield, Andrew J., Zhang, David Yu   +3 more
core   +1 more source

Epstein-Barr virus infections and DNA hybridization studies in posttransplantation lymphoma and lymphoproliferative lesions: The role of primary infection [PDF]

, 1985
Fourteen patients who developed B cell lymphomas or lymphoproliferative lesions after kidney, liver, heart, or heart-lung transplantation in Pittsburgh during 1981-1983 had active infection with Epstein-Barr virus (EBV)of the primary (six patients ...
Andiman, W, Atchison, RW, Bahnson, HT, Breinig, MK, Dummer, JS, Eastman, R, Griffith, BP, Hakala, TR, Hardesty, RL, Ho, M, Miller, G, Rosenthal, JT, Starzl, TE   +12 more
core   +1 more source

Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization.

International Journal of Systematic Bacteriology, 1999
The phylogenetic relationships of 49 Acinetobacter strains, 46 of which have previously been classified into 18 genomic species by DNA-DNA hybridization studies, were investigated using the nucleotide sequence of gyrB, the structural gene for the DNA ...
Satoshi Yamamoto, Philippe Bouvet, S. Harayama   +2 more
semanticscholar   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source