Results 61 to 70 of about 1,065,519 (331)

Fabrication of porcine DNA biosensor based on ruthenium bipyridine complex [PDF]

, 2016
Electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) as label redox complex has been developed. The system of biosensor is based on the modified screen printed carbon electrode (SPE) with goldnanoparticles ...
Abd Karim, Nurul Huda, Abdullah Halid, Nurul Izni, Ahmad, Haslina, Hasbullah, Siti Aishah, Lee, Yook Heng, Zakariah, Emma Izzati   +5 more
core   +1 more source

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

FEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]

, 1992
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson, Burke, Christoph Lengauer, Eric D. Green, Green, Kariya, Lengauer, Lengauer, Lichter, Lichter, Lichter, Nelson, Pinkel, Ried, Selleri, Thomas Cremer   +15 more
core   +1 more source

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

DNA hybridization catalysts and catalyst circuits [PDF]

, 2005
Practically all of life's molecular processes, from chemical synthesis to replication, involve enzymes that carry out their functions through the catalysis of metastable fuels into waste products.
A.J. Turberfield, B. Yurke, B. Yurke, C. Flamm, C. Green, C. Radding, C.D. Mao, C.M. Niemeyer, D.M. Wong, E. Winfree, I.G. Panyutin, I.G. Panyutin, J. Shin, M.N. Stojanovic, M.O. Magnasco, N. Carmi, R.F. Gesteland, S.W. Santoro, W.B. Sherman   +18 more
core   +1 more source

Comparing self‐reported race and genetic ancestry for identifying potential differentially methylated sites in endometrial cancer: insights from African ancestry proportions using machine learning models

Molecular Oncology, EarlyView.
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley   +1 more source

Kloning DNA Genom Pengapit Transposon dari Mutan Nonpatogenik Xanthomonas axonopodis pv. glycines M715

Hayati Journal of Biosciences, 2005
The objective of this work is to clone flanking DNA derived from Tn-5 mutagenesis of wild type strain Xanthomonas axonopodis pv. glycines as first step to clone and to identify the gene involved in pathogenicity mechanism.
ALINA AKHDIYA RUSMANA, ANTONIUS SUWANTO, BUDI TJAHJONO   +2 more
doaj   +1 more source

Genome composition of 'Elatior'-begonias hybrids analyzed by genomic in situ hybridisation [PDF]

, 2010
Interspecific hybridization of various tuberous Begonia species hybrids with Begonia socotrana results in so-called 'Elatior'-begonias hybrids (B. x hiemalis Fotsch).
Laak, W.A., Marasek Ciolakowska, A.R., Ramanna, M.S., Tuyl, J.M., van   +3 more
core   +2 more sources

MET and NF2 alterations confer primary and early resistance to first‐line alectinib treatment in ALK‐positive non‐small‐cell lung cancer

Molecular Oncology, EarlyView.
Alectinib resistance in ALK+ NSCLC depends on treatment sequence and EML4‐ALK variants. Variant 1 exhibited off‐target resistance after first‐line treatment, while variant 3 and later lines favored on‐target mutations. Early resistance involved off‐target alterations, like MET and NF2, while on‐target mutations emerged with prolonged therapy.
Jie Hu, Ning Ding, Xiaobo Xu, Yedan Chen, Yong Zhang, Jingwen Liu, Jiebai Zhou, Hairong Bao, Donghui Zhang, Yijun Song, Yang Shao, Yuanlin Song   +11 more
wiley   +1 more source

Robustness and modularity properties of a non-covalent DNA catalytic reaction [PDF]

, 2009
The biophysics of nucleic acid hybridization and strand displacement have been used for the rational design of a number of nanoscale structures and functions. Recently, molecular amplification methods have been developed in the form of non-covalent DNA
Aldaye, Clo, D. Y. Zhang, E. Winfree, Feldkamp, Flamm, Frezza, Gao, Lubrich, Marras, Puglisi, SantaLucia, Seelig, Seelig, Turberfield, Yin, Yurke, Zhang, Zhang, Zhang, Zuker   +20 more
core   +4 more sources