Results 71 to 80 of about 657,941 (288)

A Label-Free Impedimetric DNA Sensor Based on a Nanoporous SnO2 Film: Fabrication and Detection Performance

Sensors, 2015
Nanoporous SnO2 thin films were elaborated to serve as sensing electrodes for label-free DNA detection using electrochemical impedance spectroscopy (EIS). Films were deposited by an electrodeposition process (EDP).
Minh Hai Le, Carmen Jimenez, Eric Chainet, Valerie Stambouli   +3 more
doaj   +1 more source

Chloroplast and Cell Imaging at Submicron Resolution by Two-Photon Excitation [PDF]

, 2012
Novel, two-photon NIR excitation fluorescence correlation microspectroscopy tests and preliminary results were presented in this article with submicron resolution for concentrated suspensions of functional cells and chloroplast membranes.
I C. Baianu
core   +1 more source

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]

, 1992
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson, Burke, Christoph Lengauer, Eric D. Green, Green, Kariya, Lengauer, Lengauer, Lichter, Lichter, Lichter, Nelson, Pinkel, Ried, Selleri, Thomas Cremer   +15 more
core   +1 more source

Crucial parameters for precise copy number variation detection in formalin‐fixed paraffin‐embedded solid cancer samples

Molecular Oncology, EarlyView.
This study shows that copy number variations (CNVs) can be reliably detected in formalin‐fixed paraffin‐embedded (FFPE) solid cancer samples using ultra‐low‐pass whole‐genome sequencing, provided that key (pre)‐analytical parameters are optimized.
Hanne Goris, Vasiliki Siozopoulou, Léon C van Kempen, Anne Sieben, Ella Roelant, Stig Hellemans, Elyne Backx, Laure Sorber, Koen De Winne, Senada Koljenović, Karen Zwaenepoel   +10 more
wiley   +1 more source

Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense

Biosensors, 2013
An optical DNA biosensor based on fluorescence resonance energy transfer (FRET) utilizing synthesized quantum dot (QD) has been developed for the detection of specific-sequence of DNA for Ganoderma boninense, an oil palm pathogen.
Noremylia Mohd Bakhori, Nor Azah Yusof, Abdul Halim Abdullah, Mohd Zobir Hussein   +3 more
doaj   +1 more source

Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens [PDF]

, 2003
BACKGROUND: We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Blackburn, Gary F, Chan, Vivian, Chen, Yin-Peng, Farkas, Daniel H, Miller, Donna L, Reeves, William C, Unger, Elizabeth R, Vernon, Suzanne D   +7 more
core   +3 more sources

Correlation of the differential expression of PIK3R1 and its spliced variant, p55α, in pan‐cancer

Molecular Oncology, EarlyView.
PIK3R1 undergoes alternative splicing to generate the isoforms, p85α and p55α. By combining large patient datasets with laboratory experiments, we show that PIK3R1 spliced variants shape cancer behavior. While tumors lose the protective p85α isoform, p55α is overexpressed, changes linked to poorer survival and more pronounced in African American ...
Ishita Gupta, Yang Song, Madeleine Ndahayo, Anirudh Saxena, Theresa Guo, Dylan Z. Kelley, Jessica Gore, Andrew Hennigan, Alexa Anderson, John C. Papadimitriou, Daria A. Gaykalova   +10 more
wiley   +1 more source

Robustness and modularity properties of a non-covalent DNA catalytic reaction [PDF]

, 2009
The biophysics of nucleic acid hybridization and strand displacement have been used for the rational design of a number of nanoscale structures and functions. Recently, molecular amplification methods have been developed in the form of non-covalent DNA
Aldaye, Clo, D. Y. Zhang, E. Winfree, Feldkamp, Flamm, Frezza, Gao, Lubrich, Marras, Puglisi, SantaLucia, Seelig, Seelig, Turberfield, Yin, Yurke, Zhang, Zhang, Zhang, Zuker   +20 more
core   +4 more sources

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Label-free (fluorescence-free) sensing of a single DNA molecule on DNA origami using a plasmon-enhanced WGM sensor

Nanophotonics
The integration of DNA origami structures with opto-plasmonic whispering gallery mode (WGM) sensors offers a significant advancement in label-free biosensing, overcoming the limitations of traditional fluorescence-based techniques, and providing enhanced
Ghamari Shahin, Chiarelli Germán, Kołątaj Karol, Subramanian Sivaraman, Acuna Guillermo P., Vollmer Frank   +5 more
doaj   +1 more source