Results 81 to 90 of about 657,941 (288)

Mechanisms of DNA Hybridization: Transition Path Analysis of a Simulation-Informed Markov Model

, 2018
Complementary DNA strands in solution reliably hybridize to form stable duplexes. We study the kinetics of the hybridization process and the mechanisms by which two initially isolated strands come together to form a stable double helix.
Jin, Raymond, Maibaum, Lutz
core   +1 more source

Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]

, 1993
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini, ASCENZIONI, Fiorentina, DONINI, Pierluigi, T. Gallo   +3 more
core   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Sorting of chromosomes by magnetic separation [PDF]

, 1988
Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension ...
A Oberteufer, B Schw�ger, B Trask, C Cremer, Christoph Cremer, CS Owen, D Durnam, D Peters, D Pinkel, D Pinkel, Eduardo Diaz, Ernst W. Steegmayer, G Dudin, Gertrud Dudin, Haide Schnitzer, JC Fuscoe, JG Collard, K Nustad, Kathryn E. Howell, KE Howell, L Manuelidis, L-C Yu, M Schardin, Peter Vogt, PW Rigby, RS Molday, RV Lebo, T Lea, T Lea, Thomas Cremer, U Blochmann   +30 more
core   +1 more source

Quantitative evaluation of the impact of artificial cell adhesion via DNA hybridization on E-cadherin-mediated cell adhesion [PDF]

, 2020
Shodai Togo, Ken Sato, Ryuzo Kawamura, Naritaka Kobayashi, Makoto Noiri, Seiichiro Nakabayashi, Yuji Teramura, Hiroshi Yoshikawa   +7 more
openalex   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

Nanoparticle-enhanced electrochemical biosensor with DNA immobilization and hybridization of Trichoderma harzianum gene

Sensing and Bio-Sensing Research, 2014
The genus Trichoderma is a soil-borne fungi which in numerous reports has been successfully used as a biological control agent against various plant pathogens.
Shafiquzzaman Siddiquee, Kobun Rovina, Nor Azah Yusof, Kenneth Francis Rodrigues, Saallah Suryani   +4 more
doaj   +1 more source

Enhanced DNA Sensing via Catalytic Aggregation of Gold Nanoparticles [PDF]

, 2013
A catalytic colorimetric detection scheme that incorporates a DNA-based hybridization chain reaction into gold nanoparticles was designed and tested.
Graugnard, Elton, Hughes, William L., Huttanus, Herbert M., Knowlton, William B., Kuang, Wan, Lee, Jeunghoon, Yurke, Bernard   +6 more
core   +2 more sources

TMC4 localizes to multiple taste cell types in the mouse taste papillae

FEBS Open Bio, EarlyView.
Transmembrane channel‐like 4 (TMC4), a voltage‐dependent chloride channel, plays a critical role in amiloride‐insensitive salty taste transduction. TMC4 is broadly expressed in all mature taste cell types, suggesting a possible involvement of multiple cell types in this pathway.
Momo Murata, Yoshikazu Saito, Yoichi Kasahara, Wataru Fujii, Takumi Misaka, Tomiko Asakura, Masataka Narukawa   +6 more
wiley   +1 more source

The strategies of DNA immobilization and hybridization detection mechanism in the construction of electrochemical DNA sensor: A review

Sensing and Bio-Sensing Research, 2017
In recent years, electrochemical deoxyribonucleic acid (DNA) sensor has recently emerged as promising alternative clinical diagnostic devices especially for infectious disease by exploiting DNA recognition events and converting them into an ...
Jahwarhar Izuan Abdul Rashid, Nor Azah Yusof   +1 more
doaj   +1 more source