Zhongguo shiyan zhenduanxue, 2011 目的定量检测脓毒症大鼠肠道主要菌群的变化情况,同时找出引起脓毒症大鼠患病甚至死亡的主要致病菌。方法采用盲肠结扎穿孔法（CLP）制作大鼠脓毒症模型,取腹水进行细菌培养和菌种鉴定;同时取结肠内容物提取DNA,使用荧光定量PCR法对肠道内5种细菌进行定量。结果与对照组相比模型组大鼠肠道内双歧杆菌、乳杆菌数量明显减少,而大肠埃希菌的数量则有所增加。同时大肠埃希菌在腹水中大量繁殖。结论肠道中益生菌数量减少和条件致病菌的大量繁殖是导致脓毒症大鼠发病和死亡的微生态学因素。
刘大全 +4 more
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 1993 采用异硫氰酸胍、柠檬酸钠、2-巯基乙醇、十二烷基肌氨酸钠作为变性裂解剂,结合水饱和酚、氯仿、异戍醇等进行快速程序式抽提血清HBV-DNA模板用于多聚酶链式（PCR）基因扩增。该提取法比一般方法简单、稳定、不易污染,抽提过程可在lh内完成。PCR应用结果表明敏感性高于地高辛素标记探针斑点杂交法。经酶切及a-32标记放射自显影鉴定PCR产物具有良好的特异性。
doaj
Zhongguo shiyan zhenduanxue, 2014 目的探讨白细胞介素-17F基因多态性与儿童支气管哮喘的相关性,为支气管哮喘的易感基因筛选提供可能的理论依据。方法取本院门诊随诊的60例哮喘患儿设为哮喘组,60例健康儿童设为对照组,两组儿童均于清晨空腹留取外周静脉血2ml,提取全基因组DNA,应用聚合酶链反应及克隆、基因测序方法分析IL-17F基因序列rs763780（H161R）基因多态性。结果 IL-17F基因序列rs763780（H161R）基因型在两组内均未检测到突变型CC,rs763780（H161R）基因型（TC/TT ...
赵宏霞, 刘瑜, 庄哈娜
doaj
[Binary encryption comprehensive experiment based on agarose gel electrophoresis]. [PDF]
Se PuDuan JW +7 more
europepmc +1 more source
[Mefloquine HCl promotes DNA repair and alleviates radiation-induced lung epithelial cell injury]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoZhang Y, Wen Q, Huang H, Zhou M, Wang J.
europepmc +1 more source
[Diagnostic Performance of Metagenomic Next-Generation Sequencing for Mucormycosis: A Retrospective Cohort Study]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanZhang W, Zhong S, Lu S, Xiao X, Xie Y.
europepmc +1 more source
[Optimized extraction of active components of <i>Paeonia lactiflora</i> and their antioxidant, anti-inflammatory and pigmentation-reducing effects for skin whitening]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoXu Q +6 more
europepmc +1 more source
[Establishment of an Epstein-Barr virus infection model using human nasal organoids]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoWei J +5 more
europepmc +1 more source
[Analysis of the Advantages and Clinical Validation of Real-Time Fluorescence Nucleic Acid Isothermal Amplification Detection Technology in Detecting Low Viral Load Samples From HBV-Infected Patients]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanHuang Q +5 more
europepmc +1 more source
[Correlation analysis of cell-free DNA in gingival crevicular fluid with periodontal clinical indicators and cyclic guanosine phosphate-adenosine phosphate synthase-stimulator of interferon genes signaling pathway]. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiChen L, Zhu X, Zhou J, Li J, Zhao L.
europepmc +1 more source