Results 51 to 60 of about 5,601 (168)

Phylogenomic insights into the relationship and the evolutionary history of planthoppers (Insecta: Hemiptera: Fulgoromorpha) 基于基因组的系统发育分析为蜡蝉亚目(Fulgoromorpha)的系统发育关系及进化提供新见解

open access: yesSystematic Entomology, Volume 50, Issue 3, Page 495-518, July 2025.
Using genomic data from 285 species across 19 planthopper families, we conducted the first phylogenomic study targeting global planthoppers and inferred their phylogeny with the highest resolution so far. We proposed taxonomic changes including the establishment of a new family Borysthenidae stat. rev. and a new superfamily Meenoploidea superfam.
Junchen Deng   +9 more
wiley   +1 more source

Research on Biological Nitrogen Removal by Aerobic Denitrification Process at Low-temperature and the Psychrolerant Mechanism of Functional Bacteria [PDF]

open access: yes, 2015
本研究针对传统生物脱氮工艺存在能耗大、基建和运行费用高、低温运行效果差等缺陷,采用好氧反硝化工艺处理人工模拟含氮污水,实现好氧条件下同一反应器内全程生物脱氮过程。采用逐步提高曝气时间及曝气强度的方式,以KNO3为氮源,以普通城市污水处理厂的活性污泥为接种泥,实现了好氧反硝化菌的富集及工艺的常温启动。在此基础上,研究了温度对好氧反硝化工艺的性能影响,及低温环境下反应器运行条件的调控效果。此外,针对分离得到的异养硝化-好氧反硝化纯菌的耐冷特性,通过测定其在短期降温过程中ATP含量的变化情况 ...
王语颖
core  

Large seeds as a defensive strategy against partial granivory in the Fagaceae

open access: yesJournal of Ecology, Volume 113, Issue 3, Page 598-607, March 2025.
Large seeds dispersed by scatter‐hoarding granivores, such as Fagaceae acorns, tolerate partial damage to achieve higher dispersal efficiency. By demonstrating that seed size itself acts as a defensive trait, the findings provide new insights into seed size diversity and evolution, highlighting how large seeds counterbalance reserve losses to evade ...
Si‐Chong Chen   +5 more
wiley   +1 more source

一种东北虎全血DNA抽提的方法

open access: yes野生动物学报, 2007
以8只东北虎全血为材料,用CTAB自配细胞裂解液和细胞沉淀液,采用改进的方法提取基因组DNA。结果表明,提取的基因组DNA纯度高,用琼脂糖凝胶电泳检测基因组DNA,主带清晰、无拖尾现象。
黄志宏   +4 more
doaj  

Corneal neovascularization is allowed by the degradation and remodeling of typeⅠcollagen depended on Notch4-upregulated VEGFr3 expression [PDF]

open access: yes, 2015
背景:角膜是无血管的高度透明的特殊组织,承担了眼三分之二的视觉功能。角膜新生血管(Cornealneovascularization,CNV)严重威胁视力,甚至导致失明,其机制未明,被认为是新形成的血管以出芽方式从角膜缘血管丛侵入无血管的角膜基质的过程。尽管近几十年来国内外已经针对CNV进行了大量的基础和临床研究,并取得了一些进展,然而因CNV发生的原因多种多样,发病机制非常复杂,到目前为止,仍没有发现一种共同的机制可以解释不同原因导致的CNV,也没有找到一种十分有效的方法治疗CNV。因此 ...
周丹
core  

“Security, first of all, begins at home”: How Finland, Latvia, and Germany prepare “ordinary people” for crises

open access: yesEuropean Policy Analysis, Volume 11, Issue 1, Page 114-137, Winter 2025.
Abstract In Europe, and specifically in countries bordering the Baltic Sea, preparedness issues have moved up the policy agenda since Russia's 2014 and 2022 invasions of Ukraine. “Ordinary people” are encouraged to prepare for crises—be it through stockpiling food at home, fact‐checking information, or signing up for military training.
Alexandra M. Friede
wiley   +1 more source

Transcriptomic study of a toxin-producing dinoflagellate Alexandrium catenella [PDF]

open access: yes, 2015
亚历山大藻(Alexandriumspp.)是一种能够产生麻痹性贝类毒素(paralyticshellfishtoxins,PSTs)的海洋甲藻,广泛分布于近海环境中。近年来由其产生的毒素导致的海洋生物死亡及人类中毒事件频发,严重威胁到近海生态系统、水产养殖业、人类健康和生命财产安全,已成为一个全球性的环境和健康问题。虽然目前在亚历山大藻毒素来源及产毒生理方面取得了较大进展,但对毒素合成机制的了解仍较少。 本论文以有毒链状亚历山大藻(Alexandriumcatenella)为研究对象 ...
张勇
core  

反应体系对荧光定量PCR检测乙肝核酸结果影响的分析

open access: yesZhongguo shiyan zhenduanxue, 2013
目的探讨荧光定量PCR检测乙型肝炎病毒核酸(HBV-DNA)时核酸提取体系中加入样本量的不同和不同反应体系对检测结果的影响。方法选取乙肝检测患者DNA定量结果为低拷贝数(10-103copies/ml)和高拷贝数(105-107copies/ml)样本各10例,分别按2种核酸提取体系及2种反应体系分为4组进行HBV-DNA荧光定量PCR检测。结果 10例低拷贝数样本减少反应体系后只有3例检出有反应,由于反应体系的减小造成结果存在明显差异 ...
郭楠   +4 more
doaj  

改良的 Trizol 法提取小鼠皮肤 RNA 技术

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2020
【目的】由于皮肤组织韧性强且存在大量RNA酶,采用经典Trizol法提取的RNA存在易降解质量低等不足。因此,本研究旨在通过改良的Trizol法实现高质量的皮肤RNA提取。【方法】采用不同处理方式(Tri:Trizol包埋;Pro:RNA样本保护液包埋;Cry:先冷冻液氮再冷冻-80℃冰箱;LNG:液氮研磨;Cut:采用剪刀剪碎组织)提取的小鼠正常皮肤RNA为实验组;脊髓组织作为参照组,并以咪喹莫特(IMQ)诱导的小鼠银屑病模型皮肤组织作为保真性验证组。我们采用紫外分光光度法、琼脂凝胶电泳法 ...
林震嘉   +7 more
doaj  

Interference of Homologous Sequences on the SNP Study of CYP2A13 Gene [PDF]

open access: yes, 2010
Background and objective It has been proven that cytochrome P450 enzyme 2A13 (CYP2A13) played an important role in the association between single nucleotide polymorphisms (SNP) and human diseases.
Chaorong MEI   +7 more
core  

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