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Integrative Conservation, Volume 4, Issue 4, Page 527-533, December 2025.The recommendations cover four key aspects namely the definition of the taxa require high priority in the biodiversity conservation management, enhanced flexibility and adaptivity of the implementation processes, enforced integration of research advancement to enable the sustainable usage of plant resources, and finally actions to propose transboundary
Shi‐Cheng Shao +4 more
wiley +1 more source
野生动物学报, 2010 褐马鸡是中国特有的国家Ⅰ级保护濒危鸟类,为了解决野生褐马鸡分子生物学研究中存在的取样局限性,采用非伤害性取样和非损伤性取样,对褐马鸡微量血液、皮、脚垫、指甲、羽毛、陈旧标本的皮以及粪便的DNA提取进行了初步研究。结果表明,从褐马鸡微量血液（10μL）中可以提取到浓度和纯度高的基因组DNA（41.85μg）;从褐马鸡的皮、脚垫、指甲、羽毛中可以提取出基因组DNA并扩增出目标片断;陈旧标本的皮能够提取到基因组DNA。该研究将拓宽褐马鸡分子生物学研究的采样范围,并可以提高野外非损伤性取样在保护遗传学中的应用。
武玉珍, 王孟本, 张峰
doaj
Function of SAV6 in Maintenance of Genome Integrity and Root Development in Arabidopsis [PDF]
, 2015 flap核酸内切酶FEN1（flapendonuclease1）是RAD2家族的一种能够识别特异底物结构的核酶。FEN1具有三种酶活性：flap核酸内切酶（FlapEndonuclease,FEN）活性、5’核酸外切酶（5’-Exonuclease,EXO）活性和缺口依赖的核酸内切酶（GapEndonuclease,GEN）活性，在DNA复制、修复以及维持端粒稳定性等方面发挥着重要作用。人们对FEN1在哺乳动物、酵母中的功能已经有了深入了解，但是拟南芥中尚无相关研究 ...
张益娟
core
Policy Studies Journal, Volume 53, Issue 4, Page 994-1013, November 2025.Abstract Defined by threat, urgency, and uncertainty, crises produce opportunities for government leaders to exploit and create meaning around their policy decisions in such unstable circumstances. In narrating their preferred policy solutions, one of the tools governments can use is relying on evidence‐based information.
Laura Mastroianni, Stefania Profeti
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2013 目的比较两种不同方法提取的基因组DNA的纯度、产量和后续实验效果。方法采用酚/氯仿法和盐析法两种方法直接从全血中提取基因组DNA,并采用电泳、PCR和基质支持的激光释放/电离飞行时间质谱分析（MALDI-TOF MS）方法进行检测。结果酚/氯仿法提取的基因组DNA纯度高于盐析法 ...
宋洁云 +4 more
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Study on the microbial community structure, diversity and abundance in water-soil interface sediments on the watershed of three rivers [PDF]
, 2013 为了探讨无机盐分、养分及有机质矿化作用对微生物群落结构、多样性和功能微生物丰度影响，河流水土界面微生物群落结构和碳、氮、硫循环功能微生物种群的演替规律，本论文以九龙江、闽江和瓯江三江流域作为研究区域，并增加温室培养实验，对其中水土界面沉积物样品中的理化参数、微生物群落、多样性和功能微生物丰度进行测定；采用基因文库构建技术、变性梯度凝胶电泳技术（DGGE）和illumina高通量测序技术分析了样品中微生物群落结构及活动规律；利用实时荧光定量PCR（RT-PCR）技术对功能微生物进行定量 ...
李虎
core
Veterinary Dermatology, Volume 36, Issue 5, Page 668-678, October 2025.Background – Erythemato‐ceruminous otitis externa (ECOE) is commonly associated with Malassezia spp. and microbial imbalance. Hypothesis/Objective – To assess the clinical performance of an ear cleaner in dogs suffering from ECOE associated with Malassezia spp. overgrowth and to measure its impact on the microbiota. Conclusions and Clinical Relevance –
Amaury Briand +11 more
wiley +1 more source
Research on the Scheme of Medical Virtual Experiment Platform Based on Virtools [PDF]
, 2013 本论文从国内外医科院校实验教学的趋势出发，着重阐述了虚拟仿真实验室的研究内涵、背景现状、及其具体运用中的利与弊，展望虚拟仿真实验室在医学实验教学中的具体应用前景，探索如何利用虚拟仿真实验室更有效提升教学资源品质，进一步提高教学质量更高效完成教学任务。努力打造更加规范、科学的医学实验技能培训平台，为医学精品课程建设提供有效的教学题材补充。 随着教育信息技术的飞速发展，特别是网络技术的深入应用，为推动高校实验教学的信息化教学提供了有力的技术支撑 ...
曾爱兵
core
Integrative Conservation, Volume 4, Issue 3, Page 277-286, September 2025.This study provides an innovation to address the challenge in accessibility to samples and genetic markers in Snow leopard conservation genetic studies. We performed next‐generation sequencing (NGS) on 19 fecal samples obtained from the eastern Qinghai‐Tibet Plateau, and successfully assembled 17 complete mitogenome sequences (~16,720 bp) and ...
Chen Cheng +7 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2003 目的 探讨石蜡包埋组织中提取DNA应用于PCR反应的影响因素。方法 应用从石蜡包埋组织中提取的DNA ,PCR反应扩增甲状腺髓样癌RET基因第 11外显子 ,分析石蜡包埋组织中提取DNA应用于PCR反应的影响因素。结果 10 .0 μm× 1组织切片组 ,PCR反应循环次数 4 0次组 ,PCR反应模板量 0 .0 5 μg组PCR反应取得高质量目的基因DNA。结论 减少提取组织用量有利于减少抑制PCR物质的量 ,有利于反应的成功 ;PCR反应循环次数一般应大于 4 0次 ...
赵吉生 +6 more
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