Results 11 to 20 of about 5,601 (168)
Veterinary Dermatology, EarlyView.ABSTRACT Background Advances in transcriptomics have driven the demand for minimally invasive, reproducible and high‐yield skin sampling methods, particularly for studying inflammatory skin diseases in companion animals. Hypothesis/Objectives We tested tolerability, feasibility and RNA quantity and quality of three minimally invasive skin sampling ...
Ina Herrmann +2 more
wiley +1 more source
Functional Ecology, Volume 40, Issue 5, Page 1428-1442, May 2026.Read the free Plain Language Summary for this article on the Journal blog. Abstract The stoichiometric imbalance caused by nitrogen (N) deposition typically exacerbates phosphorus (P) limitation in plants. However, it remains unclear whether this effect extends to soil microbes, particularly those in the rhizosphere.
Jipeng Wang +5 more
wiley +1 more source
Functional Ecology, Volume 40, Issue 5, Page 1415-1427, May 2026.Read the free Plain Language Summary for this article on the Journal blog. Abstract Wildfires are disrupting and regenerating forces in nature that affect all ecosystem components. However, the impact of wildfires on soil multitrophic biodiversity and the resulting consequences for ecosystem multifunctionality (EMF) remain poorly understood.
Juan Zhou +6 more
wiley +1 more source
Rare Metals, Volume 45, Issue 4, April 2026.ABSTRACT Piezocatalytic technology utilizes mechanical strain or stress‐induced piezoelectric phenomena to produce robust internal electric fields, markedly improving the separation efficiency of e−‐h+ pairs. This method has emerged as a focal point in catalysis by removing reliance on conventional light or electrical energy via its mechanical‐to ...
Yue Zhou +5 more
wiley +1 more source
Functional Ecology, Volume 40, Issue 3, Page 636-654, March 2026.Read the free Plain Language Summary for this article on the Journal blog. Abstract The nested subset pattern (nestedness) has been widely used to explain species distributions in island and fragmented systems. Mountain sky islands serve as critical natural laboratories for understanding the evolutionary consequences of geographic isolation and climate
Caiwen Zhang +5 more
wiley +1 more source
Advances in Elemene Nanodelivery Systems: From Material Design to Disease Treatment
Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT Elemene (ELE) is a bioactive sesquiterpenoid extracted from traditional Chinese herbs, demonstrating broad‐spectrum antitumor, anti‐inflammatory, and analgesic properties with significant therapeutic potential. However, its clinical utility is constrained by inherent physicochemical limitations, including volatility and hydrophobicity, which ...
Xiao Wang +9 more
wiley +1 more source
Genetic mapping and quantitative trait loci analysis of growth-related traits in the small abalone Haliotis diversicolor using next-generation sequencing [PDF]
, 2016 杂色鲍(Haliotisdiversicolor)广泛分布于韩国、日本、中国、越南和菲利宾沿岸，是一种具有重要经济价值的养殖鲍种。应用传统的育种方式，杂色鲍育种工作虽然已经取得了一定进展，但是开展分子标记辅助选育仍然是加速该进程的必然选择。下一代测序技术可以高通量、低成本地完成SNP标记的发掘与分型，目前已被广泛应用于遗传研究的各个方面。本文利用下一代测序技术完成了杂色鲍的SNP标记开发与验证、高密度连锁图谱构建、QTL定位和候选基因的关联分析等工作。主要结果如下： (1) 杂色鲍高密度连锁图谱的构建 ...
任鹏
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Zhongguo shiyan zhenduanxue, 2006 目的为寻找一种高效、简便、实用的石蜡组织中DNA提取方法,本研究比较了三种不同的DNA提取方法对DNA质量的影响。方法选取淋巴相关组织的蜡块21例。三种DNA提取方法分别是简单消化煮沸法,酚/氯仿提纯法和试剂盒法。通过提取DNA的浓度和纯度、PCR扩增保守序列β-globin等方法,比较三种DNA提取方法的优劣。结果试剂盒法提取样品OD260/OD280平均比值位于1.61.8之间;提纯法位于1.31.5之间;简单消化煮沸法位于1.21.3之间。三种方法中,试剂盒法提取的DNA最为稳定 ...
齐宗利 +4 more
doaj
Zhejiang Daxue xuebao. Lixue ban, 2013 为了建立一种检测水中铜绿微囊藻浓度的定量PCR方法，分别用SDS裂解法和DNeasy Blood & Tissue Kit试剂盒提取标准品的DNA并建立标准曲线.结果表明采用DNeasy Blood & Tissue Kit试剂盒提取的标准品DNA具有很好的重复性，经定量PCR扩增得到的标准曲线R2为0.968，可以用来检测样品中铜绿微囊藻的浓度，为建立一套长期有效的测定水中铜绿微囊藻浓度的监测体系提供了技术参考.
ZHUJin-yu(朱金余) +3 more
doaj +1 more source
The genetic differentiation and mating system of Avicennia marina populations in China [PDF]
, 2016 本研究利用SSR分子标记技术，以海南三亚河口、湛江特呈岛和泉州洛阳桥三个地区的白骨壤(Avicenniamarina)种群为研究对象，比较分析三个白骨壤种群的遗传多样性、种群之间的遗传分化和繁育系统模式(包括花粉流、异交率和种子流)。主要结果为： 1、10对核基因组SSR引物在742个个体检测到58个等位基因位点。每个引物扩增等位基因数在5-9个之间，平均5.8个。三个地区种群共出现了15个私有等位基因位点，在等位基因水平上已发现较大的分化与遗传距离。 2、湛江白骨壤种群遗传多样性水平最高 ...
侯蕊
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