Results 21 to 30 of about 2,140,063 (119)
Rare Metals, Volume 45, Issue 6, June 2026.ABSTRACT Lung cancer is one of the most common and deadly malignancies worldwide. Although immune checkpoint inhibitors, including PD‐1/PD‐L1 blockers, have benefited a subset of patients, response rates remain limited, and recurrence is frequent because of the immunosuppressive tumor microenvironment (TME), characterized by hypoxia and dysregulated ...
Yi Ma +6 more
wiley +1 more source
福尔马林固定白鱀豚标本DNA提取及其遗传多样性的初步研究 [PDF]
, 2005 福尔马林固定标本是宝贵的遗传资源,但是如何有效利用其中的遗传信息一直存在问题。本文尝试从标本预处理、消化、PCR扩增各方面综合考虑和优化改进,成功提取并扩增21头福尔马林固定白豚标本线粒体DNA控制区410bp片段。采用了3种预处理方法尽量去除固定标本中残存的甲醛,从试验结果来看,从酒精梯度+临界点干燥处理的标本中提取的DNA在扩增时具有明显优势。通过蛋白酶K消化过程中对于酶的浓度、温浴时间的比较试验,发现随着采用大幅提高酶浓度、延长消化时间等高强度的蛋白酶消化操作后 ...
许黎美,万秋红,王丁
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Rare Metals, Volume 45, Issue 6, June 2026.ABSTRACT Metal‐organic frameworks (MOFs) hold significant potential across numerous advanced disciplines due to their exceptional properties, including high specific surface area, tunable porosity, structural diversity, and customizable functionality. Increasing attention has been directed toward employing MOFs in coatings that offer both antimicrobial
Yanchun Zhao +7 more
wiley +1 more source
Functional Ecology, Volume 40, Issue 6, Page 1921-1932, June 2026.Read the free Plain Language Summary for this article on the Journal blog. Abstract Most terrestrial plant species form symbioses with arbuscular mycorrhizal fungi (AMF). However, the below‐ground carbon (C) allocation of plants and the nutritional and growth benefits provided by AMF within this symbiosis vary greatly across species and environments ...
Hengjun Zhao +3 more
wiley +1 more source
Veterinary Dermatology, Volume 37, Issue 3, Page 375-385, June 2026.ABSTRACT Background Advances in transcriptomics have driven the demand for minimally invasive, reproducible and high‐yield skin sampling methods, particularly for studying inflammatory skin diseases in companion animals. Hypothesis/Objectives We tested tolerability, feasibility and RNA quantity and quality of three minimally invasive skin sampling ...
Ina Herrmann +2 more
wiley +1 more source
Functional Ecology, Volume 40, Issue 5, Page 1428-1442, May 2026.Read the free Plain Language Summary for this article on the Journal blog. Abstract The stoichiometric imbalance caused by nitrogen (N) deposition typically exacerbates phosphorus (P) limitation in plants. However, it remains unclear whether this effect extends to soil microbes, particularly those in the rhizosphere.
Jipeng Wang +5 more
wiley +1 more source
Functional Ecology, Volume 40, Issue 5, Page 1415-1427, May 2026.Read the free Plain Language Summary for this article on the Journal blog. Abstract Wildfires are disrupting and regenerating forces in nature that affect all ecosystem components. However, the impact of wildfires on soil multitrophic biodiversity and the resulting consequences for ecosystem multifunctionality (EMF) remain poorly understood.
Juan Zhou +6 more
wiley +1 more source
, 2008 本实验采用EP管反复冻融和研磨的破壁方式,利用溶菌酶法、CTAB法、改良CTAB法、尿素法、十二烷基硫酸钠(SDS)法等5种方法,分别对5种鱼类致病性水霉菌(寄生水霉、多子水霉、异株水霉及两未定种)的基因组DNA进行提取,并采用紫外分光光度计和ITS区基因(包括5.8SrDNA)PCR扩增对DNA进行了评价。紫外分光光度计检测结果表明,5种方法均可提取到水霉菌DNA,其中改良CTAB法提取的5种水霉菌的DNA产量和质量最高,A260/A280在1.79—1.82之间,浓度为45μg/mL ...
顾泽茂 +4 more
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Rare Metals, Volume 45, Issue 4, April 2026.ABSTRACT Piezocatalytic technology utilizes mechanical strain or stress‐induced piezoelectric phenomena to produce robust internal electric fields, markedly improving the separation efficiency of e−‐h+ pairs. This method has emerged as a focal point in catalysis by removing reliance on conventional light or electrical energy via its mechanical‐to ...
Yue Zhou +5 more
wiley +1 more source
, 2014 本发明公开了一种易变山羊草的咖啡因提取物及其提取方法与应用，属于生物工程领域。本发明中易变山羊草的咖啡因提取物的提取方法的具体步骤如下：取易变山羊草根部清洗后切成片，在80℃烘箱中烘干6小时彻底去除水分，然后用粉碎机粉碎，过60目筛后，把10克粉碎的易变山羊草根部样放入50毫升的萃取釜中，采用无水乙醇作为夹带剂，在CO2提取仪中30MPa，60℃萃取2h，其中CO2流量控制在1.5LPM，获得易变山羊草的咖啡因提取物。本发明研究表明＞0.1％浓度的咖啡因对植物病原线虫有生长有抑制作用 ...
郑明辉 +6 more
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