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PLANTS, PEOPLE, PLANET, Volume 8, Issue 1, Page 301-315, January 2026.Mango, the king of fruits, is important to daily life, nutrition, and households in South and Southeast Asia. It is considered the second most important fruit in Taiwan, where many varieties are available. Using high‐throughput DNA genotyping, we analyzed mango germplasm for variety identification, genebank management, and breeding. Our results confirm
Ching‐heng Lin +5 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2018 目的探讨在高海拔地区应用血液基因组DNA提取试剂盒的最佳条件。方法应用RelaxGene Blood DNA System血液基因组DNA提取系统,对109份血样(每份血样分为2组,实验组和对照组)提取DNA,实验组采用优化条件提取DNA、对照组采用试剂盒说明书方法提取DNA。结果实验组109份样品中有13份DNA浓度在20-50ng/μL之间,35份在51-80ng/μL之间,61份样品>80ng/μL;DNA浓度比例高于对照组 ...
范东艳, 杜宝中, 王苹, 全雄杰
doaj
A rapid soil DNA extraction method applied to field detection(一种适用于现场检测的土壤DNA快速提取方法(英文))
浙江大学学报. 农业与生命科学版Soil DNA extraction, such as microbial community analysis and gene drift detection, is an important basis for multiple analyses in different fields. Nevertheless, the soil DNA extraction methods for field detection are still lacking.
YAN Jingying(颜晶莹) +3 more
doaj +1 more source
Integrative Conservation, Volume 4, Issue 4, Page 569-583, December 2025.Accurate species identification is critical for conserving endangered Firmiana trees in China. However, morphological similarities among closely related Firmiana species complicate its taxonomic identification. We conducted genome skimming on 62 samples representing all 10 Chinese species and discovered nrITS as the optimal DNA barcode, achieving 100 ...
Li‐Jun Yan +8 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2006 目的比较三种快速、简便、适用、低成本的从全血中提取基因组DNA的方法。方法选用快速氯仿-异戊醇法、简化盐析法、改良碘化钾法直接从全血中提取基因组DNA。结果改良碘化钾法提取的DNA纯度及含量最高,快速氯仿-异戊醇法次之,简化盐析法则相对较低。结论改良碘化钾法为较好的提取基因组DNA的方法。
涂向东, 江清华, 兰风华
doaj
Screening and Characteristics of Bacteria and Enzyme for Degradation of Malachite Green from Marine Sediment [PDF]
, 2015 孔雀石绿是一种三苯基甲烷类染料，广泛应用于水产、纺织、造纸等产业，导致水生环境化学需氧量(COD)和生物需氧量(BOD)大幅提高，阻挡光的透射，严重影响水生生物的生长，同时其毒性和致癌性也会通过食物链传递给人类及其他陆生生物。本研究以深海和红树林沉积物中微生物为研究对象，采用富集培养和宏基因组技术对可降解孔雀石绿的微生物及功能基因资源进行挖掘，并对其降解途径及代谢产物进行研究，为近海环境修复提供潜在的、可应用的生物资源 ...
曲武
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The Genome of Phoebe zhennan (Lauraceae) and Comparative Genomics of Phoebe 楠木(樟科)基因组与楠属植物比较基因组学研究
Integrative Conservation, Volume 4, Issue 4, Page 584-599, December 2025.Phoebe zhennan is a Chinese endemic and valuable timber species of Phoebe (Lauraceae). We present a chromosome‐level genome assembly of P. zhennan. Our research provides novel insights into the genome evolution of Phoebe, including whole‐genome duplication, phylogenetic relationships of magnoliids, and key MYB regulatory genes involved in lignin ...
Mao Yameng +3 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2009 目的通过比较两种从石蜡包埋组织中提取基因组DNA的方法,确定一种高效、经济而简便的方法指导实际工作。方法按照两种方法（脱蜡法与非脱蜡法）提取DNA,比较所提DNA的质量,PCR扩增β-Globin基因作为基因分析的对照扩增,并对其中非脱蜡法进行改良。结果两种方法所提取的DNA均能达到相关分子学实验的要求,PCR扩增带清晰,结果较为相似。结论非脱蜡法也能提取出适用于PCR技术要求的DNA,方法简便、经济。
王卡娜 +4 more
doaj
野生动物学报, 2020 毛发（无根）作为一种非损伤性的生物样本，在保护遗传学和分子生态学研究中发挥着十分重要的作用。以毛干中提取的DNA为模板做PCR扩增时，mtDNA片段扩增效果较好，但核基因的扩增成功率极低。人们将其归因于核DNA质低量少所致。然而我们在前期研究中发现，从毛干中得到的总DNA混有大量的细菌DNA，是导致核基因扩增困难的主要因素之一。为此我们尝试建立一种高效、快速、低成本，而且不影响DNA提取的清除毛干细菌的方法。本研究以蓝狐被毛为材料，经过普通清洗后，以4种方法处理毛干，随后使用磁珠法进行总DNA提取 ...
周永恒 1 马跃 1 +3 more
doaj
Microeukaryotic community structure and diversity in Xiamen offshore waters and Shenhu Bay, Fujian [PDF]
, 2015 微型真核浮游生物是海洋生态系统的重要组成成分，同时在海洋经典食物链和微食物网中发挥重要作用。该类群个体较小，对环境变化较为敏感，作为生产者、消费者和分解者的微型真核浮游生物在海洋生态系统的生物地球化学过程和生态健康评价等方面有着重要意义。本研究选择厦门岛近海海域和福建省泉州市深沪湾为例，对目标海区进行微型真核浮游生物样品的釆集，运用变性梯度凝胶电泳（DGGE）、克隆测序和高通量测序技术方法对微型真核浮游生物群落组成和多样性进行研究，分析其时空分布和群落结构特征 ...
于凌宇
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