Results 91 to 100 of about 5,163,408 (306)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Different binding properties and function of CXXC zinc finger domains in Dnmt1 and Tet1 [PDF]

, 2011
Several mammalian proteins involved in chromatin and DNA modification contain CXXC zinc finger domains. We compared the structure and function of the CXXC domains in the DNA methyltransferase Dnmt1 and the methylcytosine dioxygenase Tet1.
Rottach Andrea, Frauer, C., Wang Mengxi, Meilinger, D., Spada, Fabio, Andrea Rottach, Leonhardt, Heinrich, Weihua Qin, Qin, W., Frauer, Carina, Bultmann, Sebastian, Daniela Meilinger, Frauer Carina, Fellinger, K., Qin Weihua, Söding Johannes, Rottach, A., Hasenöder, Stefan, Qin, Weihua, Leonhardt Heinrich, Spada, F., Spada Fabio, Hasenoeder, S., Heinrich Leonhardt, Fellinger, Karin, Meilinger Daniela, Stefan Hasenöder, Mengxi Wang, Johannes Söding, Wang, M., Fabio Spada, Fellinger Karin, Söding, J., Hasenöder Stefan, Meilinger, Daniela, Söding, Johannes, Wang, Mengxi, Bultmann Sebastian, Bultmann, S., Rottach, Andrea, Leonhardt, H., Wutz, Anton, Carina Frauer, Sebastian Bultmann, Karin Fellinger   +44 more
core   +2 more sources

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Specificity of Baculorivus P6.9 Basic DNA-Binding Proteins and Critical Role of the C Terminus in Virion Formation [PDF]

, 2010
The majority of double-stranded DNA (dsDNA) viruses infecting eukaryotic organisms use host- or virus-expressed histones or protamine-like proteins to condense their genomes.
Wang, Hualin, Tuladhar, Era, Shu Shen, Just M. Vlak, Wang, Manli, Shen, S., van Oers, M.M., Wang, H., Monique M. van Oers, Vlak, J.M., Manli Wang, Westenberg, Marcel, van Oers, Monique M., Wang, M., Tuladhar, E., Marcel Westenberg, Westenberg, M., Vlak, Just M., Hualin Wang, Oers, M.M., van, Shen, Shu, Era Tuladhar   +21 more
core   +1 more source

Affinity-based enrichment strategies to assay methyl-CpG binding activity and DNA methylation in early Xenopus embryos

BMC Research Notes, 2011
Background DNA methylation is a widespread epigenetic modification in vertebrate genomes. Genomic sites of DNA methylation can be bound by methyl-CpG-binding domain proteins (MBDs) and specific zinc finger proteins, which can recruit co-repressor ...
Bogdanović Ozren, Veenstra Gert Jan C
doaj   +1 more source

An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1

BMC Cancer, 2020
Background Inhibition of DNA-binding of proteins by small-molecule chemicals holds immense potential in manipulating the activities of DNA-binding proteins.
Manthan Patel, Divyesh Patel, Subhamoy Datta, Umashankar Singh   +3 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Optimising DNA binding to carbon nanotubes by non-covalent methods [PDF]

, 2011
The use of carbon nanotubes as a gene delivery system has been extensively studied in recent years owing to its potential advantages over viral vectors.
Coley, Helen M., Silva, S. Ravi P., Sanz, Vanessa, Flahaut, E, Ewa Borowiak, Petar Lukanov, Vanesa Sanz, Galibert, Anne-Marie, Johnjoe McFadden, Emmanuel Flahaut, Lukanov, Petar, Helen M. Coley, Coley, HM, Borowiak, E, Flahaut, Emmanuel, Lukanov, P, McFadden, J, S. Ravi P. Silva, McFadden, Johnjoe, Borowiak-Palen, Ewa, Sanz, V, Anna Marie Galibert, Silva, SRP, Galibert, AM   +23 more
core   +1 more source