Results 91 to 100 of about 1,668,835 (315)

Different binding properties and function of CXXC zinc finger domains in Dnmt1 and Tet1 [PDF]

, 2011
Several mammalian proteins involved in chromatin and DNA modification contain CXXC zinc finger domains. We compared the structure and function of the CXXC domains in the DNA methyltransferase Dnmt1 and the methylcytosine dioxygenase Tet1.
Rottach Andrea, Frauer, C., Wang Mengxi, Meilinger, D., Spada, Fabio, Andrea Rottach, Leonhardt, Heinrich, Weihua Qin, Qin, W., Frauer, Carina, Bultmann, Sebastian, Daniela Meilinger, Frauer Carina, Fellinger, K., Qin Weihua, Söding Johannes, Rottach, A., Hasenöder, Stefan, Qin, Weihua, Leonhardt Heinrich, Spada, F., Spada Fabio, Hasenoeder, S., Heinrich Leonhardt, Fellinger, Karin, Meilinger Daniela, Stefan Hasenöder, Mengxi Wang, Johannes Söding, Wang, M., Fabio Spada, Fellinger Karin, Söding, J., Hasenöder Stefan, Meilinger, Daniela, Söding, Johannes, Wang, Mengxi, Bultmann Sebastian, Bultmann, S., Rottach, Andrea, Leonhardt, H., Wutz, Anton, Carina Frauer, Sebastian Bultmann, Karin Fellinger   +44 more
core   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Affinity-based enrichment strategies to assay methyl-CpG binding activity and DNA methylation in early Xenopus embryos

BMC Research Notes, 2011
Background DNA methylation is a widespread epigenetic modification in vertebrate genomes. Genomic sites of DNA methylation can be bound by methyl-CpG-binding domain proteins (MBDs) and specific zinc finger proteins, which can recruit co-repressor ...
Bogdanović Ozren, Veenstra Gert Jan C
doaj   +1 more source

Structural organization of DNA in chlorella viruses. [PDF]

PLoS ONE, 2012
Chlorella viruses have icosahedral capsids with an internal membrane enclosing their large dsDNA genomes and associated proteins. Their genomes are packaged in the particles with a predicted DNA density of ca. 0.2 bp nm(-3).
Timo Wulfmeyer, Christian Polzer, Gregor Hiepler, Kay Hamacher, Robert Shoeman, David D Dunigan, James L Van Etten, Marco Lolicato, Anna Moroni, Gerhard Thiel, Tobias Meckel   +10 more
doaj   +1 more source

The detection of DNA-binding proteins by protein blotting

Nucleic Acids Research, 1980
A method, called "protein blotting," for the detection of DNA-binding proteins is described. Proteins are separated on an SDA-polyacrylamide gel. The gel is sandwiched between 2 nitrocellulose filters and the proteins allowed to diffuse out of the gel and onto the filters.
Bowen, Brian, Steinberg, Jay, Laemmli, Ulrich Karl, Weintraub, Harold   +3 more
openaire   +3 more sources

Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase [PDF]

, 2013
Uracil-DNA glycosylase (UDG) compromises the replication strategies of diverse viruses from unrelated lineages. Virally encoded proteins therefore exist to limit, inhibit or target UDG activity for proteolysis.
Cole, Ambrose R., Savva, Renos, Baltulionis, Gediminas, Ofer, S, Hornyak, P, Ryzhenkova, K, Baltulionis, G, Cole, AR, Ofer, Sapir, Savva, R, Ryzhenkova, Ksenia, Hornyak, Peter   +11 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Optimising DNA binding to carbon nanotubes by non-covalent methods [PDF]

, 2011
The use of carbon nanotubes as a gene delivery system has been extensively studied in recent years owing to its potential advantages over viral vectors.
Coley, Helen M., Silva, S. Ravi P., Sanz, Vanessa, Flahaut, E, Ewa Borowiak, Petar Lukanov, Vanesa Sanz, Galibert, Anne-Marie, Johnjoe McFadden, Emmanuel Flahaut, Lukanov, Petar, Helen M. Coley, Coley, HM, Borowiak, E, Flahaut, Emmanuel, Lukanov, P, McFadden, J, S. Ravi P. Silva, McFadden, Johnjoe, Borowiak-Palen, Ewa, Sanz, V, Anna Marie Galibert, Silva, SRP, Galibert, AM   +23 more
core   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1

BMC Cancer, 2020
Background Inhibition of DNA-binding of proteins by small-molecule chemicals holds immense potential in manipulating the activities of DNA-binding proteins.
Manthan Patel, Divyesh Patel, Subhamoy Datta, Umashankar Singh   +3 more
doaj   +1 more source