Results 111 to 120 of about 5,403,999 (308)

The ubiquitin system in normal and infected germinal center B cells

FEBS Letters, EarlyView.
The ubiquitin system plays a central role in germinal center (GC) B cells, influencing differentiation to long‐lived memory B cells. Oncogenic gammaherpesviruses gain access to memory B cells by establishing latency in GC B cells. Mapping ubiquitin mechanisms in normal and infected GC B cells will define specific molecular circuits in B cells germane ...
Destiny Davis, Lesly Mejia, Emily K Moser   +2 more
wiley   +1 more source

The dynamics of replication licensing in live Caenorhabditis elegans embryos [PDF]

, 2012
Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of ...
Blow, J. Julian; id_orcid, Remi Sonneville, Matthieu Querenet, Sonneville, Remi, J. Julian Blow, Anton Gartner, Querenet, Matthieu, Blow, J. Julian, Gartner, Anton, Craig, Ashley, Ashley Craig   +10 more
core   +1 more source

Identifying the “stripe” transcription factors and cooperative binding related to DNA methylation

Communications Biology
DNA methylation plays a critical role in gene regulation by modulating the DNA binding of transcription factors (TFs). This study integrates TFs’ ChIP-seq profiles with WGBS profiles to investigate how DNA methylation affects protein interactions ...
Ximei Luo, Quan Zou
doaj   +1 more source

Metastatic niche shaped by host factors influences disseminated cancer cell fate

FEBS Letters, EarlyView.
Metastasis is shaped not only by cancer cells but also by the environments they encounter. This review explores how factors such as aging, diet, the microbiome, lifestyle, and environmental exposures remodel organ‐specific niches in the lung, liver, bone, and brain, influencing where metastatic cells survive, remain dormant, or grow, and ultimately ...
Gwennan Delyth Ward, Álvaro Ruiz‐Tabas, Patricia Altea‐Manzano   +2 more
wiley   +1 more source

Molecular basis for SMC rod formation and its dissolution upon DNA binding. [PDF]

, 2015
SMC condensin complexes are central modulators of chromosome superstructure in all branches of life. Their SMC subunits form a long intramolecular coiled coil, which connects a constitutive "hinge" dimerization domain with an ATP-regulated "head ...
Soh, Young-Min, Stephan Gruber, Kim, Cheolhee, Kim, Y.-G., Kim, YG, Oh, B., Bürmann, F., Soh, Y., Shin, H.-C., Oh, Byung-Ha, Shin, HC, Lee, H., Durand-Diebold, M., Jin, KS, S. Gruber, Lee, Hansol, Kim, Ho Min, Shin, Ho-Chul, Kim, HM, Buermann, Frank, Toseland, C., Kim, H.M., Oda, T, Soh, YM, Durand-Diebold, Marie-Laure, -Diebold, ML, Kim, Y., Gruber, S., Jin, Kyeong Sik, Bürmann, Frank, Oh, BH, Durand-Diebold, M.-L., Kim, Yeon-Gil, Kong, Min-Seok, Kim, C, Oda, Takashi, C. Kim, Lee, Nam Ki, M-L Durand-Diebold, Lee, N.K., Kim, H., Kim, Soo Jin, Sato, M., Gruber, S, Toseland, Christopher P., Oda, T., Lee, NK, M-S Kong, Diebold-Durand, Marie-Laure, Lee, N., Y.-G. Kim, Kim, C., Toseland, C.P., Soh, Young Min, Kim, Ho-Min, Gruber, Stephan, Soh, Y.-M., Oh, B.-H., Sato, Mamoru, Buermann, F., Kim, S., Jin, K.S., Shin, H., Kim, S.J., Kong, M.-S., Kong, M., S. J. Kim, Burmann, F, Jin, K., C. P. Toseland   +69 more
core   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Metal complexes as DNA intercalators [PDF]

, 2011
DNA has a strong affinity for many heterocyclic aromatic dyes, such as acridine and its derivatives. Lerman in 1961 first proposed intercalation as the source of this affinity, and this mode of DNA binding has since attracted considerable research ...
Peter J. Sadler, Hong-Ke Liu, Sadler, P. J., Liu, Hong-Ke   +3 more
core   +1 more source

The detection of DNA-binding proteins by protein blotting

Nucleic Acids Research, 1980
A method, called "protein blotting," for the detection of DNA-binding proteins is described. Proteins are separated on an SDA-polyacrylamide gel. The gel is sandwiched between 2 nitrocellulose filters and the proteins allowed to diffuse out of the gel and onto the filters.
Bowen, Brian, Steinberg, Jay, Laemmli, Ulrich Karl, Weintraub, Harold   +3 more
openaire   +3 more sources

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source