Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
The extreme C terminus of herpes simplex virus DNA polymerase is crucial for functional interaction with processivity factor UL42 and for viral replication [PDF]
, 1993 The herpes simplex virus DNA polymerase is composed of two subunits, a large catalytic subunit (Pol) and a smaller subunit (UL42) that increases the processivity of the holoenzyme.
Bebrin, William R +8 more
core
Development of methods for the analysis of deep sequencing data; applications to the discovery of functions of RNA-binding proteins [PDF]
, 2014 With the recent advances in nucleotide sequencing technologies, it became easy to generate tens of millions of reads with genome- or transcriptome-wide distribution with reduced cost and high accuracy.
Bilen, Biter
core +1 more source
FEBS Letters, EarlyView.The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile +8 more
wiley +1 more source
Harnessing DNA Binding Proteins from Starved Cells for DNA Origami Protection
Small StructuresDNA origami is an attractive method for building structurally versatile and addressable nanostructures for diverse applications. The structural integrity of DNA, however, is rather limited in application‐relevant environments, such as in low‐magnesium ...
Sharon Saarinen +6 more
doaj +1 more source
Nucleic acid - protein fingerprints. Novel protein classification based on nucleic acid - protein recognition [PDF]
, 2012 Protein chemistry uses protein description and classification based on molecular mass and isoelectric point as general features. Enzymes are also compared by enzymatic reaction constants, namely Km and kcat values. Proteins are also studied by binding to
Renad Zhdanov, Alexander Krylov
core +1 more source
Visualizing helicases unwinding DNA at the single molecule level. [PDF]
, 2010 DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali +17 more
core +1 more source
FEBS Letters, EarlyView.Although too much iron in the brain promotes neurodegeneration, iron ion chelators have had mixed effects in clinical trials. This review explains why; some chelators do not render the iron redox‐inactive (e.g., L1) whereas others do (e.g., desferrioxamine).
Barry Halliwell
wiley +1 more source
Identifying the “stripe” transcription factors and cooperative binding related to DNA methylation
Communications BiologyDNA methylation plays a critical role in gene regulation by modulating the DNA binding of transcription factors (TFs). This study integrates TFs’ ChIP-seq profiles with WGBS profiles to investigate how DNA methylation affects protein interactions ...
Ximei Luo, Quan Zou
doaj +1 more source
Contributions of ubiquitin- and PCNA-binding domains to the activity of Polymerase η in Saccharomyces cerevisiae [PDF]
, 2007 Bypassing of DNA lesions by damage-tolerant DNA polymerases depends on the interaction of these enzymes with the monoubiquitylated form of the replicative clamp protein, PCNA.
Bielen, Aleksandra B. +3 more
core +1 more source