Results 131 to 140 of about 3,048,093 (298)

Biochemical studies with DNA polymerase beta and DNA polymerase beta-PAK of Trypanosoma cruzi suggest the involvement of these proteins in mitochondrial DNA maintenance

, 2008
Mammalian DNA polymerase p is a nuclear enzyme involved in the base excision and single-stranded DNA break repair pathways. in trypanosomatids, this protein does not have a defined cellular localization, and its function is poorly understood.
Regis-da-Silva, Carlos Gustavo, Fonseca Schamber-Reis, Bruno Luiz, DaRocha, Wanderson Duarte, Rajao, Matheus Andrade, Franco, Gloria Regina, Schenkman, Sergio [UNIFESP], Cazaux, Christophe, Ribeiro Teixeira, Santuza Maria, Macedo, Andrea Mara, Hoffmann, Jean-Sebastein, Lopes, Debora de Oliveira, Machado, Carlos Renato, Junho Pena, Sergio Danilo, Nardelli, Sheila Cristina [UNIFESP]   +13 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

DNA-directed DNA polymerase and strand displacement activity of the reverse transcriptase encoded by the R2 retrotransposon.

Journal of Molecular Biology, 2007
A. Kurzyńska-Kokorniak, Varuni K Jamburuthugoda, Arkadiusz Bibillo, T. Eickbush   +3 more
semanticscholar   +1 more source

Metal- and DNA-binding properties and mutational analysis of the transcription activating factor, B, of coliphage 186: A prokaryotic C4 zinc- finger protein [PDF]

, 1997
Coliphage 186 B is a 72-amino acid protein belonging to the Ogr family of analogous transcription factors present in P2-like phage, which contain a Cys-X2-Cys-X22-Cys-X4-Cys presumptive zinc-finger motif.
Tiwari, Ravi P., Pountney, D.L., Egan, J.B., Pountney, Dean Louis, Egan, John Barry, Tiwari, R.P.   +5 more
core  

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase

BioTechniques, 2005
Yair Adereth, Kristen J. Champion, Tien Hsu, Vincent Dammai   +3 more
doaj   +1 more source

Transcription-coupled nucleotide excision repair and its regulation by the DNA damage checkpoint [PDF]

, 2009
Elaborate DNA repair mechanisms have evolved, allowing cells to repair damages in their genomes. Nucleotide excision repair (NER) removes a variety of helix-distorting lesions, including those caused by ultraviolet (UV) irradiation.
Taschner, M.J.
core  

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source