Results 111 to 120 of about 249,414 (217)

Viral Infection‐Inspired Autonomous Detection of Fusion‐Competent Viruses for Screening and Environmental Surveillance

Advanced Materials, EarlyView.
Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park, Yidam Song, Hyun‐Woo Choi, Jaeuk Sung, Harin Jin, WonSeok Gwak, Kirim Yoo, Soohwang Lee, Jongeon Park, Jeongmin Kim, Hye‐Jun Jo, Jahyun Koo, Youngdo Jeong, Kwan Hyi Lee, Seung‐Jung Kee, Hojun Kim   +15 more
wiley   +1 more source

Efficient genome editing with chimeric oligonucleotide-directed editing. [PDF]

Nat Commun
Nguyen LT, Rakestraw NR, Pizzano BLM, Iyyappan R, Young CB, Huang Y, Beerensson KT, Fang A, Antal SG, Anamisis KV, Peggs CMD, Yan J, Jing Y, Lewis JG, Burdine RD, Adamson B, Jiang Z, Toettcher JE, Myhrvold C, Jain PK.   +19 more
europepmc   +1 more source

Intimate Interaction Between Nucleic Acid and Conjugated Polymers in Organic Electrochemical Transistors Enables Ultrasensitive Biomarker Detection

Advanced Materials, EarlyView.
The intimate interaction between negatively charged RNA molecules and conjugated polymers in organic electrochemical transistors (OECTs) in aqueous electrolytes is investigated. It demonstrates that RNA binding reduces the volumetric capacitance of the polymer channel, enabling the development of an ultrasensitive biosensing platform capable of ...
Hong Liu, Naixiang Wang, Anneng Yang, Jiajun Song, Li Li, Iain McCulloch, Helen Ka‐wai Law, Feng Yan   +7 more
wiley   +1 more source

Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system. [PDF]

Nat Microbiol
Ong S, Ghode P, Narenderan A, Lao S, Willenborg F, Eden TV, Marsh CO, Yew WS, Fredens J.   +8 more
europepmc   +1 more source

Sensitive and Spatially‐Resolved Electrochemiluminescence via Micropatterning

Advanced Materials Interfaces, EarlyView.
The use of a simple silica nanoparticle surface coating and patterning process can transform ordinary gold electrodes into multiplexed ECL sensing electrodes. Through spatially confined droplet formation and local ECL emission, it is possible to perform multiple analyses simultaneously from individual locations as well as intensify the ECL signal by as
Yuliang Shao, Tatiana Starikova, Mengzhen Xi, Maria Vittoria Balli, Emanuele Luigi Sciuto, Gabriele Giagu, Vincenzo Paratore, Carmelo Corsaro, Enza Fazio, Sabrina Conoci, Luca Prodi, Giovanni Valenti, Pavel A. Levkin   +12 more
wiley   +1 more source

Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells. [PDF]

Proc Natl Acad Sci U S A
Sabol AL, Mengiste AA, Singh P, Sreekanth V, Hendel SJ, Tran MTN, Barybin AM, Chaudhary S, Harris RM, Liivak KE, Severance ZC, Locicero CM, Kailass K, Lee C, Xu LQ, Butty VL, Choudhary A, Shoulders MD.   +17 more
europepmc   +1 more source

Chromosomal Instability Drives Glioblastoma Heterogeneity and Therapeutic Opportunities

Advanced Science, EarlyView.
ABSTRACT Glioblastoma, the most aggressive and lethal form of brain cancer, is defined by profound genomic instability, with Chromosomal Instability (CIN) playing a central role in driving tumor progression, therapy resistance, and poor prognosis. CIN is characterized by numerical and structural alterations, is driven by mechanisms such as mitotic ...
Amarnath Pal, Milan Patra, Rianita Mondal, Shubhasis Haldar   +3 more
wiley   +1 more source

Correction: P3 site-directed mutagenesis: An efficient method based on primer pairs with 3'-overhangs. [PDF]

J Biol Chem
Mousavi N, Zhou E, Razavi A, Ebrahimi E, Varela-Castillo P, Yang XJ.   +5 more
europepmc   +1 more source

QBP1 Peptide as a Potential Anti‐Amyloidogenic Therapy for Type 2 Diabetes: An In Vitro Study

Advanced Science, EarlyView.
The anti‐amyloidogenic peptide QBP1 effectively halts human islet amyloid polypeptide (hIAPP) aggregation, preventing the formation of toxic β‐structured intermediates. Through a combination of biophysical assays, molecular dynamics, and cell‐based studies, QBP1 is shown to preserve β‐cell viability and metabolic homeostasis, positioning it as a ...
María M. Tejero‐Ojeda, Ada Bernaus Vives, Michał Wojciechowski, Dinh Quoc Huy Pham, Mateusz Chwastyk, Mario Vallejo, Anna Novials, Douglas V. Laurents, Mariano Carrión‐Vázquez   +8 more
wiley   +1 more source

Opposing regulation by Rev1 of DNA polymerase zeta activity on damaged versus undamaged DNA. [PDF]

Nucleic Acids Res
Bezalel-Buch R, Stith C, Makarova AV, Binz SK, Burgers PM.   +4 more
europepmc   +1 more source