Results 91 to 100 of about 410,825 (308)

Characterization of the Products of RNA-directed DNA Polymerases in Oncogenic RNA Viruses

Nature, 1970
Several RNA tumour viruses contain an enzyme that synthesizes a DNA–RNA hybrid using the single stranded viral RNA as template. Hybridization experiments confirm that the DNA strand is complementary to the viral RNA.
Spiegelman, S., Burny, Arsène, Das, M R, Keydar, J., Schlom, J., Travnicek, M., Watson, Kenneth M.   +6 more
openaire   +3 more sources

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Stress tolerance-related genetic traits of fish pathogen Flavobacterium psychrophilum in a mature biofilm [PDF]

, 2018
Indexación: Scopus.Flavobacterium psychrophilum is the causative agent of bacterial cold-water disease and rainbow trout fry syndrome, and hence this bacterium is placed among the most important salmonid pathogens in the freshwater aquaculture industry ...
Avendaño-Herrera, R., Levipan, H.A., Quezada, J.   +2 more
core   +1 more source

DragonRNA: Generality of DNA-primed RNA-extension activities by DNA-directed RNA polymerases

Nucleic Acids Research
Abstract RNA polymerases (RNAPs) transcribe DNA into RNA. Several RNAPs, including from bacteriophages Sp6 and T7, Escherichia coli, and wheat germ, had been shown to add ribonucleotides to DNA 3′ ends. Mitochondria have their own RNAPs (mtRNAPs).
Emily Greenwald, Drew Galls, Joon Park, Nimit Jain, Stephen B Montgomery, Bijoyita Roy, Y Whitney Yin, Andrew Z Fire   +7 more
openaire   +2 more sources

EDNRB‐dependent endothelin signaling reduces proliferation and promotes proneural‐to‐mesenchymal transition in gliomas

Molecular Oncology, EarlyView.
Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau, Leonor Garcia, Hugo Arnold, Antonija Hanžek, Maialen Arrieta, Laurent R Gauthier, Christine Granotier‐Beckers, François D Boussin, Amaury Herbet, Marie Hautière, Valentin Asei‐Ceschino, Clémentin Jacques, Laura Brard, Thomas Harnois, Valérie Coronas, Bruno Constantin, Aurélien Chatelier, Jean Chemin, Serge Urbach, Martial Seveno, Szimonetta Hideg, Chantal Ripoll, Kasandra Aguilar‐Cázarez, Min Zheng, Guo‐Hao Huang, Sheng‐Qing Lv, Lei Zhang, Philippe Rondard, Laurent Prezeau, Jean‐Philippe Pin, Hugues Duffau, Luc Bauchet, Valérie Rigau, Franck Denat, Charles Truillet, Didier Boquet, Jean‐Philippe Hugnot   +36 more
wiley   +1 more source

Genomic context influences translesion synthesis DNA polymerase-dependent mechanisms of micronuclei induction by G-quadruplexes

Cell Reports
Summary: Guanine quadruplexes (G4s) are non-canonical DNA structures that can trigger micronuclei (MNi). Mechanisms of micronuclei formation by G4s are not fully understood.
Simona Pepe, Federico Guerra, Marco Russo, Renée C. Duardo, Giovanni Capranico   +4 more
doaj   +1 more source

Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis. [PDF]

, 2013
DNA methylation occurs in CG and non-CG sequence contexts. Non-CG methylation is abundant in plants and is mediated by CHROMOMETHYLASE (CMT) and DOMAINS REARRANGED METHYLTRANSFERASE (DRM) proteins; however, its roles remain poorly understood.
Do, Truman, Du, Jiamu, Feng, Suhua, Jacobsen, Steven E, Johnson, Lianna, Patel, Dinshaw J, Stroud, Hume, Zhong, Xuehua   +7 more
core   +2 more sources

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Annotation of genomics data using bidirectional hidden Markov models unveils variations in Pol II transcription cycle

Molecular Systems Biology, 2014
DNA replication, transcription and repair involve the recruitment of protein complexes that change their composition as they progress along the genome in a directed or strand‐specific manner.
Benedikt Zacher, Michael Lidschreiber, Patrick Cramer, Julien Gagneur, Achim Tresch   +4 more
doaj   +1 more source

An Iterative and Regenerative Method for DNA Sequencing

BioTechniques, 1997
This paper presents, to our knowledge, the first iterative DNA sequencing method that regenerates the product of interest during each iterative cycle, allowing it to overcome the critical obstacles that impede alternative iterative approaches to DNA ...
Douglas H. Jones
doaj   +1 more source