Results 241 to 250 of about 2,860,424 (274)

On the Binding of Rifampicin to the DNA‐Directed RNA Polymerase from Escherichia coli

European Journal of Biochemistry, 1973
The capacity of RNA polymerase to bind the antibiotic rifampicin is rather sensitive to conditions which lead to conformational changes in the enzyme. Repeated freezing and thawing in 1 M LiCl as well as treatment with guanidinium chloride destroys the binding affinity of the enzyme for the inhibitor. Similarly rifampicin does not associate with any of
U I, Lill, G R, Hartmann
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Mitochondrial DNA-directed RNA polymerase from Saccharomyces cerevisae mitochondria

Biochemical and Biophysical Research Communications, 1971
Abstract A DNA-directed RNA polymerase activity has been detected in yeast mitochondrial extracts which is sensitive to rifampicin. This activity is distinct from that found in the nucleus and cytoplasm, and is possibly coded for, or under the control of the mitochondrial genome.
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The effect of aryl hydrocarbon receptor ligands on the expression of polymerase (DNA directed) kappa (Polκ), polymerase RNA II (DNA directed) polypeptide A (PolR2a), CYP1B1 and CYP1A1 genes in rat liver

Environmental Toxicology and Pharmacology, 2012
The aryl hydrocarbon receptor (AhR) mediates a variety of biological responses to ubiquitous environmental pollutants. AhR is ligand activated transcription factor with high affinities for aromatic planar compounds such as β-naphthoflavone (BNF), 3-methylcholanthrene (3-MC), benzo[a]pyrene (BaP) or dioxin (TCDD).
Damian, Brauze, Agnieszka Anna, Rawłuszko   +1 more
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The isolation and properties of a DNA-directed RNA polymerase from yeast mitochondria

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
A method is described for the rapid isolation of yeast mitochondrial DNA-directed RNA polymerase. The enzyme obtained had a specific activity of 1.56 nmol UMP incorporated per mg protein in 20 min at 37 degrees C, and is some 95% pure. This purified enzyme upon polyacrylamide gel elctrophoresis consists of a single polypeptide of 68 000 mol.
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Mode of inhibition of HIV-1 reverse transcriptase by polyacetylenetriol, a novel inhibitor of RNA- and DNA-directed DNA polymerases

Biochemical Journal, 2002
Polyacetylenetriol (PAT), a natural marine product from the Mediterranean sea sponge Petrosia sp., was found to be a novel general potent inhibitor of DNA polymerases. It inhibits equally well the RNA- and DNA-dependent DNA polymerase activities of retroviral reverse transcriptases (RTs) (i.e.
Shoshana, Loya, Amira, Rudi, Yoel, Kashman, Amnon, Hizi   +3 more
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DNA and RNA polymerases

Polymerases are enzymes that catalyze the synthesis of long chains of nucleic acids. This chapter focuses on DNA and RNA polymerases expressed in different organisms, which assemble DNA and RNA molecules (respectively) by copying a nucleic acid template ...
Mattia Mori, Francesca Picarazzi
exaly   +2 more sources

Lysine Methylation Mapping of Crenarchaeal DNA-Directed RNA Polymerases by Collision-Induced and Electron-Transfer Dissociation Mass Spectrometry

Journal of Proteome Research, 2014
Enzymatic machineries fundamental for information processing (e.g., transcription, replication, translation) in Archaea are simplified versions of their eukaryotic counterparts. This is clearly noticeable in the conservation of sequence and structure of corresponding enzymes (see for example the archaeal DNA-directed RNA polymerase (RNAP)). In Eukarya,
Mikel, Azkargorta, Magdalena N, Wojtas, Nicola G A, Abrescia, Felix, Elortza   +3 more
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DNA-directed RNA polymerase from HeLa cells

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
Bernd J. Benecke, Klaus H. Seifart
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DNA-directed RNA polymerase from HeLa cells. Isolation, characterization and cell-cycle distribution of three enzymes.

Biochimica et biophysica acta, 1976
DNA-directed RNA polymerase was solubilized from total HeLa cells. Three distinct classes of the enzyme could be clearly differentiated by their sensitivity toward alpha-amanitin. While form A is completely resistant to high concentrations (133 mug/ml) of this toxin, enzyme B is highly sensitive and is completely inhibited by concentrations of 0.1 mug ...
B J, Benecke, K H, Seifart
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DNA-directed RNA polymerase

1997
Dietmar Schomburg, Dörte Stephan
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