Results 71 to 80 of about 3,029,744 (275)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Implications of ZNF334 gene in lymph node metastasis of lung SCC: potential bypassing of cellular senescence

Journal of Translational Medicine
Background The primary goal of this work is to identify biomarkers associated with lung squamous cell carcinoma and assess their potential for early detection of lymph node metastasis.
Khalil Khashei Varnamkhasti, Mehdi Moghanibashi, Sirous Naeimi   +2 more
doaj   +1 more source

Discovery of cyanophage genomes which contain mitochondrial DNA polymerase [PDF]

, 2011
DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that
Whitworth, Anna L., Holmes, Antony B., Mohr, Remus, Hess, Wolfgang R., Nicholas H. Mann, David J. Scanlan, Remus Mohr, Scanlan, David J., Antony B. Holmes, Anthony W. Larkum, Martha R. J. Clokie, Clokie, Martha R. J., Anna L. Whitworth, Yi-Wah Chan, Millard, Andrew D., Wolfgang R. Hess, Mann, Nicholas H., Andrew D. Millard, Chan, Yi-Wah, Larkum, Anthony W. D., Mohr, R, Hess, Wolfgang, Millard, AD, Larkum, Anthony W., Scanlan, DJ, Clokie, MRJ, Chan, YW, Hess, WR, Larkum, AW, Mann, NH, Whitworth, AL, Holmes, AB   +31 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Novel platform for DNA alignment and studying DNA-protein interaction in realtime [PDF]

, 2011
Venkatesh A. Novel platform for DNA alignment and studying DNA-protein interaction in realtime. Bielefeld: Universität; 2011.Recreating DNA-protein interaction in vitro could unravel the relationship between their structure and function, which ...
Venkatesh, Alagarswamy
core  

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Directed evolution of T7 RNA polymerase variants using an 'autogene'

, 2004
Natural enzymes, when used in biotechnological applications are often found not well suited for the tasks. Enzyme properties can now be improved by rational design or by directed evolution to produce useful biocatalysts.
Chelliserrykattil, Jijumon Pavithran, 1974-
core  

Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism [PDF]

, 2010
For a long time it has been assumed that the decay of RNA in eukaryotes is mainly carried out by exoribonucleases, which is in contrast to bacteria, where endoribonucleases are well documented to initiate RNA degradation.
Tomecki, Rafał, Dziembowski, Andrzej
core   +3 more sources

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes. [PDF]

, 1976
Includes bibliographical references.An RNA-dependent RNA polymerase from ribosomes of maturing chicken erythrocytes was investigated. Concurrent with an increase in globin and RNA synthesis during phenylhydrazine induced anaemia, the total and specific ...
Boyd, Malcolm Charles David
core   +1 more source