Results 71 to 80 of about 2,860,424 (274)

DNA-Directed DNA Polymerases Evolve From Reverse Transcriptase [PDF]

, 2013
Scientists have been debating for decades the origin of life on earth. A number of hypotheses were proposed as to what emerged first RNA or DNA; with most scientists are in favour of the "RNA World" hypothesis.
Zhang, Lei
core   +1 more source

Elongator protein 3b negatively regulates ribosomal DNA transcription in african trypanosomes. [PDF]

, 2011
Eukaryotic cells limit ribosomal DNA (rDNA) transcription by RNA polymerase I (RNAP-I) to maintain genome integrity. African trypanosomes present an excellent model for studies on RNAP-I regulation because they possess a bifunctional RNAP-I and because ...
Horn, David, Horn, David; id_orcid, Sam Alsford, Alsford, Sam, David Horn   +4 more
core   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Distinct roles of non-canonical poly(A) polymerases in RNA metabolism [PDF]

, 2009
Trf4p and Trf5p are non-canonical poly(A) polymerases and are part of the heteromeric protein complexes TRAMP4 and TRAMP5 that promote the degradation of aberrant and short-lived RNA substrates by interacting with the nuclear exosome. To assess the level
Schenk, L., Keller Walter, Vanacova, S., Blank Diana, Scherrer, T., San Paolo, Salvatore, Keller, W., André P Gerber, San Paolo, S., Gerber André P., Salvatore San Paolo, Tanja Scherrer, Diana Blank, Blank, Diana, Gerber, A. P., Blank, D., Scherrer Tanja, Gerber, André P., Schenk Luca, Walter Keller, Luca Schenk, Scherrer, Tanja, Vanacova Stepanka, Stepanka Vanacova, Vanacova, Stepanka, Paolo Salvatore San, Keller, Walter, Schenk, Luca   +27 more
core   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Dynamics of DNA replication loops reveal temporal control of lagging-strand synthesis [PDF]

, 2009
In all organisms, the protein machinery responsible for the replication of DNA, the replisome, is faced with a directionality problem. The antiparallel nature of duplex DNA permits the leading-strand polymerase to advance in a continuous fashion, but ...
Richardson, Charles C.,, Takahashi, Masateru, Vanoijen, Antoine M., Hamdan, Samir M., Joseph J. Loparo, Loparo, Joseph J., Richardson, Charles C., Takahashi, Masateru,, Oijen, Antoine M. van, Oijen, Antoine M. van,, Loparo, Joseph J.,, Antoine M. van Oijen, Samir M. Hamdan, Masateru Takahashi, Hamdan, Samir M.,, Charles C. Richardson   +15 more
core   +3 more sources

Inhibition of cyclin‐dependent kinases 12/13 using CT7439 as a treatment for colorectal cancer with CDK12 upregulation

Molecular Oncology, EarlyView.
The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington, Divya L. Dayanidhi, Mohammad Zokaasadi, John B. Mantyh, Pelumi D. Olawuni, Gabrielle Rupprecht, Jeremy M. Force, Shannon McCall, Ashwani K. Bahl, David S. Hsu, Jason A. Somarelli   +10 more
wiley   +1 more source

Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway [PDF]

, 2012
tRNA precursors, which are transcribed by RNA polymerase III, undergo end-maturation, splicing and base modifications. Hypomodified tRNAs, such as tRNAVal(AAC), lacking 7-methylguanosine and 5-methylcytidine modifications, are subject to degradation by a
Boguta, Magdalena, Turowski, Tomasz W., Karkusiewicz, Iwona, Kowal, J   +3 more
core   +1 more source

Epigenetic silencing of the liver‐specific lncRNA LUNAR promotes liver cancer progression via NOTCH activation

Molecular Oncology, EarlyView.
LUNAR is a liver‐specific long noncoding RNA (lncRNA) that is highly expressed in normal liver but becomes epigenetically silenced in hepatocellular carcinoma through promoter hypermethylation. Loss of LUNAR is associated with NOTCH activation, epithelial–mesenchymal transition, and metastasis, whereas restoring LUNAR restrains metastatic progression ...
Se Ha Jang, Hyung Seok Kim, Geum Ok Baek, Moon Gyeong Yoon, Su In Lee, Jee‐Yeong Jeong, Ji Eun Han, Soon Sun Kim, Jae Youn Cheong, Jung Woo Eun   +9 more
wiley   +1 more source

Base-specific spin-labeling of RNA for structure determination [PDF]

, 2007
To facilitate the measurement of intramolecular distances in solvated RNA systems, a combination of spin-labeling, electron paramagnetic resonance (EPR), and molecular dynamics (MD) simulation is presented. The fairly rigid spin label 2,2,5,5-tetramethyl-
Olav Schiemann, Mu, Yuguang, Prisner, Thomas F., Stock, G., Engels, J.W., Prisner, TF., Thomas F. Prisner, Engels, Joachim W. (Prof. Dr.), Stock, Gerhard, Piton, N., Schiemann, Olav, Gerhard Stock, Nelly Piton, Piton, Nelly, Mu, Y., Yuguang Mu, Joachim W. Engels   +16 more
core   +1 more source