Results 111 to 120 of about 7,795,985 (403)

CRISPAltRations: A validated cloud-based approach for interrogation of double-strand break repair mediated by CRISPR genome editing

Molecular Therapy: Methods & Clinical Development, 2021
CRISPR systems enable targeted genome editing in a wide variety of organisms by introducing single- or double-strand DNA breaks, which are repaired using endogenous molecular pathways.
Gavin Kurgan, Rolf Turk, Heng Li, Nathan Roberts, Garrett R. Rettig, Ashley M. Jacobi, Lauren Tso, Morgan Sturgeon, Massimo Mertens, Roel Noten, Kurt Florus, Mark A. Behlke, Yu Wang, Matthew S. McNeill   +13 more
doaj  

Metabolic engineering with ATP-citrate lyase and nitrogen source supplementation improves itaconic acid production in Aspergillus niger

Biotechnology for Biofuels, 2019
Background Bio-based production of organic acids promises to be an attractive alternative for the chemicals industry to substitute petrochemicals as building-block chemicals.
Abeer H. Hossain, Roy van Gerven, Karin M. Overkamp, Peter S. Lübeck, Hatice Taşpınar, Mustafa Türker, Peter J. Punt   +6 more
doaj   +1 more source

Loop-mediated isothermal amplification of DNA.

Nucleic Acids Research, 2000
We have developed a novel method, termed loop-mediated isothermal amplification (LAMP), that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions.
T. Notomi, H. Okayama, Harumi Otawara-shi Masubuchi, Toshihiro Yonekawa, Keiko Watanabe, N. Amino, T. Hase   +6 more
semanticscholar   +1 more source

Methylation of the imprinted GNAS1 gene in cell-free plasma DNA : equal steady-state quantities of methylated and unmethylated DNA in plasma [PDF]

, 2008
Background Genomic DNA sequences in cell-free plasma are biomarkers of cancer prognosis, where characteristic changes in methylation of tumour suppressor or oncogene DNA regions are indicative of changes in gene activity.
Alberry, Anker, Bauer, Bianchi, Birch, Chim, Deligezer, Eggermann, Francesco Crea, Grunau, Honda, Jahr, Januchowski, Kamaljit Chatha, Kawakami, Li, Lo, Lui, Robert W. Old, Selina Elsenheimer, Sorenson, Stroun, Stroun, Tsang, Van der Schoot, Vasioukhin, William M. Puszyk   +26 more
core   +1 more source

The apo LETM1 F‐EF‐hand adopts a closed conformation that underlies a multi‐modal sensory role in mitochondria

FEBS Letters, EarlyView.
We present the first solution structure of the Ca2+‐depleted LETM1 F‐EF‐hand through a D676A/N678A Ca2+ binding‐deficient mutant, revealing a closed hydrophobic cleft caused by a unique F1‐helix pivot. The apo LETM1 F‐EF‐hand exhibits regiospecific hot and cold unfolding, sensitivity to physiological pH changes and potential for promiscuous heterotypic
Qi‐Tong Lin, Danielle M. Colussi, Peter B. Stathopulos   +2 more
wiley   +1 more source

One generic synonym and one new species of Phlaeothripidae from India (Thysanoptera)

ZooKeys, 2018
Haplothrips shivendraii Tyagi & Kumar, sp. n. is described from Rajasthan state of India. The monobasic Austro-oriental genus Dyothrips Kudô is formally synonymised with Haplothrips.
Kaomud Tyagi, Devkant Singha, Goutam Kumar Saha, Vikas Kumar   +3 more
doaj   +3 more sources

New insights on the mechanism of quinoline-based DNA methyltransferase inhibitors [PDF]

, 2014
Among the epigenetic marks, DNA methylation is one of the most studied. It is highly deregulated in numerous diseases, including cancer. Indeed, it has been shown that hypermethylation of tumor suppressor genes promoters is a common feature of cancer ...
Arimondo, Paola B., Ausseil, Frédéric, Bouhlel, Mohamed Amine, Cantagrel, Frédéric, David Cordonnier, Marie Hélène, Dufau, Isabelle, Faux, Céline, Fleury, Laurence, Gros, Christina, Labella, Donatella, Lacroix, Laurent, MAI, Antonello, Mourey, Lionel, Nahoum, Virginie, Rilova, Elodie, VALENTE, Sergio   +15 more
core   +1 more source

DNA Damage, Mutagenesis, and DNA Repair [PDF]

Journal of Nucleic Acids, 2010
This special issue of the Journal of Nucleic Acids is dedicated to DNA damage and two important biological consequences provoked by such damage: lesion repair and lesion-induced mutagenesis. These phenomena have attracted broad interest among a large community of scientists that cross disciplines from mathematics, physics, chemistry, and biology to ...
Suse Broyde, Ashis K. Basu, Shigenori Iwai, Caroline Kisker   +3 more
openaire   +4 more sources

Structural and mechanistic basis for the regulation of the chloroplast signal recognition particle by (p)ppGpp

FEBS Letters, EarlyView.
LHCPs are transported to the thylakoid membrane via the (cp)SRP pathway. This process involves a transit complex of (cp)SRP43, (cp)SRP54 and LHCP, which interacts with (cp)FtsY and Alb3 at the membrane. GTP hydrolysis by (cp)SRP54 and (cp)FtsY triggers complex dissociation.
Victor Zegarra, Paul Weiland, Pauline Anka Plitzko, Julia Thiery, Laura Czech, Felix Willmund, Patricia Bedrunka, Gert Bange   +7 more
wiley   +1 more source

SET1/MLL family of proteins: functions beyond histone methylation

Epigenetics, 2021
The SET1 family of enzymes are well known for their involvement in the histone 3 lysine 4 (H3K4) methylation, a conserved trait of euchromatin associated with transcriptional activation.
Jeyapal Sugeedha, Jyoti Gautam, Shweta Tyagi   +2 more
doaj   +1 more source