Results 261 to 270 of about 4,554,738 (310)
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Journal of the American Chemical Society, 2004
Cloning DNA typically involves the joining of target DNAs with vector constructs by enzymatic ligation. A commonly used enzyme for this reaction is bacteriophage T4 DNA ligase, which requires ATP as the energy source to catalyze the otherwise unfavorable formation of a phosphodiester bond.
Alavattam, Sreedhara +2 more
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Cloning DNA typically involves the joining of target DNAs with vector constructs by enzymatic ligation. A commonly used enzyme for this reaction is bacteriophage T4 DNA ligase, which requires ATP as the energy source to catalyze the otherwise unfavorable formation of a phosphodiester bond.
Alavattam, Sreedhara +2 more
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DNA topoisomerases and DNA repair
BioEssays, 1988AbstractDNA topoisomerases are enzymes that can modify, and may regulate, the topological state of DNA through concerted breaking and rejoining of the DNA strands. They have been believed to be directly involved in DNA excision repair, and perhaps to be required for the control of repair as well. The vicissitudes of this hypothesis provide a noteworthy
C S, Downes, R T, Johnson
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DNA Methyltransferases and DNA Damage
2022Ever since the discovery of depletion of CG sites in mammalian genomes it has been clear that cytosine DNA methyltransferases (DNMTs) are linked to the rate at which mutations accumulate in DNA. Research in the intervening decades has shown that DNMTs influence mutation rates through the indirect consequences of methylation on the mechanism of mutation
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Current Protocols in Molecular Biology, 2008
AbstractThis unit presents characteristics and reaction conditions of the DNA‐dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic
Rebecca B, Kucera, Nicole M, Nichols
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AbstractThis unit presents characteristics and reaction conditions of the DNA‐dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic
Rebecca B, Kucera, Nicole M, Nichols
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DNA Methylation and DNA Structure
1984Novel DNA substrates, such as hemimethylated DNA, supercoiled, partially or fully relaxed DNA, either intact or UV-irradiated, have been used to study either some aspects of the mechanism of DNA methylation or some aspects of the effect of pathological and physiological DNA methylation on DNA conformation.
S, Spadari +5 more
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DNA Elasticity from Short DNA to Nucleosomal DNA
The Journal of Physical Chemistry B, 2015Active biological processes like transcription, replication, recombination, DNA repair, and DNA packaging encounter bent DNA. Machineries associated with these processes interact with the DNA at short length (
Garai, Ashok +3 more
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Forensic Science, Medicine, and Pathology, 2007
The rapid development, success, and occasional failures of forensic DNA profiling are highly publicised, and as a consequence are well known to the scientific and public communities alike. Over the same period of time that forensic DNA typing has accelerated onto the scene, another related discipline has been born and has made equally, or perhaps, even
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The rapid development, success, and occasional failures of forensic DNA profiling are highly publicised, and as a consequence are well known to the scientific and public communities alike. Over the same period of time that forensic DNA typing has accelerated onto the scene, another related discipline has been born and has made equally, or perhaps, even
openaire +2 more sources
Biopolymers, 2004
AbstractTargeting double‐stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA‐PNA chimeras. A 16‐mer 5′‐DNA‐3′‐p‐(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and ...
PETRACCONE, LUIGI +7 more
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AbstractTargeting double‐stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA‐PNA chimeras. A 16‐mer 5′‐DNA‐3′‐p‐(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and ...
PETRACCONE, LUIGI +7 more
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DNA Gyrase and the Supercoiling of DNA
Science, 1980Negative supercoiling of bacterial DNA by DNA gyrase influences all metabolic processes involving DNA and is essential for replication. Gyrase supercoils DNA by a mechanism called sign inversion, whereby a positive supercoil is directly inverted to a negative one by passing a DNA segment through a transient double-strand break.
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