Results 311 to 320 of about 6,548,876 (337)
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Biochemistry, 2000
Twelve classes of deoxyribozymes that promote an ATP-dependent "self-capping" reaction were isolated by in vitro selection from a random-sequence pool of DNA. Each deoxyribozyme catalyzes the transfer of the AMP moiety of ATP to its 5'-terminal phosphate group, thereby forming a 5',5'-pyrophosphate linkage.
and Yong Liu +2 more
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Twelve classes of deoxyribozymes that promote an ATP-dependent "self-capping" reaction were isolated by in vitro selection from a random-sequence pool of DNA. Each deoxyribozyme catalyzes the transfer of the AMP moiety of ATP to its 5'-terminal phosphate group, thereby forming a 5',5'-pyrophosphate linkage.
and Yong Liu +2 more
openaire +3 more sources
Current Opinion in Structural Biology, 1998
The forces that govern DNA double helix organization are being finally systematically measured. The non-specific longer-range interactions--such as electrostatic interactions, hydration, and fluctuation forces--that treat DNA as a featureless rod are reasonably well recognized.
Helmut H. Strey +3 more
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The forces that govern DNA double helix organization are being finally systematically measured. The non-specific longer-range interactions--such as electrostatic interactions, hydration, and fluctuation forces--that treat DNA as a featureless rod are reasonably well recognized.
Helmut H. Strey +3 more
openaire +3 more sources
Journal of the American Chemical Society, 2004
Cloning DNA typically involves the joining of target DNAs with vector constructs by enzymatic ligation. A commonly used enzyme for this reaction is bacteriophage T4 DNA ligase, which requires ATP as the energy source to catalyze the otherwise unfavorable formation of a phosphodiester bond.
Alavattam Sreedhara +2 more
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Cloning DNA typically involves the joining of target DNAs with vector constructs by enzymatic ligation. A commonly used enzyme for this reaction is bacteriophage T4 DNA ligase, which requires ATP as the energy source to catalyze the otherwise unfavorable formation of a phosphodiester bond.
Alavattam Sreedhara +2 more
openaire +2 more sources
Journal of Natural Resources and Life Sciences Education, 2003
This lesson discusses what DNA is and how it relates to genes and chromosomes. How and why DNA is extracted in the genetic engineering process is also covered. Overview and Objectives - DNA and DNA Extraction This lesson discusses what DNA is and how it relates to genes and chromosomes. How and why DNA is extracted in the genetic engineering process is
Hain, Patricia, Lee, Don
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This lesson discusses what DNA is and how it relates to genes and chromosomes. How and why DNA is extracted in the genetic engineering process is also covered. Overview and Objectives - DNA and DNA Extraction This lesson discusses what DNA is and how it relates to genes and chromosomes. How and why DNA is extracted in the genetic engineering process is
Hain, Patricia, Lee, Don
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DNA topoisomerases and DNA repair
BioEssays, 1988AbstractDNA topoisomerases are enzymes that can modify, and may regulate, the topological state of DNA through concerted breaking and rejoining of the DNA strands. They have been believed to be directly involved in DNA excision repair, and perhaps to be required for the control of repair as well. The vicissitudes of this hypothesis provide a noteworthy
Robert T. Johnson, C. S. Downes
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DNA Elasticity from Short DNA to Nucleosomal DNA
The Journal of Physical Chemistry B, 2015Active biological processes like transcription, replication, recombination, DNA repair, and DNA packaging encounter bent DNA. Machineries associated with these processes interact with the DNA at short length (
Garai, Ashok +3 more
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Current Protocols in Molecular Biology, 2008
AbstractThis unit presents characteristics and reaction conditions of the DNA‐dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic
Rebecca Kucera, Nicole M. Nichols
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AbstractThis unit presents characteristics and reaction conditions of the DNA‐dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic
Rebecca Kucera, Nicole M. Nichols
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Annual Review of Biochemistry, 1981
The various problems of disentangling DNA strands or duplexes in a cell are all rooted in the double-helical structure of DNA. Three distinct subfamilies of enzymes, known as the DNA topoisomerases, have evolved to solve these problems. This review focuses on work in the past decade on the mechanisms and cellular functions of these enzymes.
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The various problems of disentangling DNA strands or duplexes in a cell are all rooted in the double-helical structure of DNA. Three distinct subfamilies of enzymes, known as the DNA topoisomerases, have evolved to solve these problems. This review focuses on work in the past decade on the mechanisms and cellular functions of these enzymes.
openaire +4 more sources
DNA Methylation and DNA Structure
1984Novel DNA substrates, such as hemimethylated DNA, supercoiled, partially or fully relaxed DNA, either intact or UV-irradiated, have been used to study either some aspects of the mechanism of DNA methylation or some aspects of the effect of pathological and physiological DNA methylation on DNA conformation.
A. Rebuzzini +5 more
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