Results 341 to 350 of about 7,795,985 (403)

Using quality improvement methodology to reduce Did Not Attends in healthcare within a UK prison. [PDF]

BMJ Open Qual
Lakhman N, Harleston S, Knight E, Bodnar C, Addis F, Bheenick V.   +5 more
europepmc   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

FEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

A microfluidic platform for extraction and analysis of bacterial genomic DNA.

Lab Chip
Joesaar A, Holub M, Lutze L, Emanuele M, Kerssemakers J, Pabst M, Dekker C.   +6 more
europepmc   +1 more source

The molecular properties of the bHLH TCF4 protein as an intrinsically disordered hub transcription factor. [PDF]

Cell Commun Signal
Sozańska N, Klepka BP, Niedzwiecka A, Zhukova L, Dadlez M, Greb-Markiewicz B, Ożyhar A, Tarczewska A.   +7 more
europepmc   +1 more source

Bioengineering facets of the tumor microenvironment in 3D tumor models: insights into cellular, biophysical and biochemical interactions

FEBS Open Bio, EarlyView.
The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik, Deniz Bakkalci, Alexander J. MacRobert, Umber Cheema   +3 more
wiley   +1 more source

Comparative Analysis of Pasture Composition: DNA Metabarcoding Versus Quadrat-Based Botanical Surveys in Experimental Grassland Plots. [PDF]

Ecol Evol
Vallin H, Hipperson H, Titěra J, Jones L, Fraser M.   +4 more
europepmc   +1 more source

Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS‐PAGE with low acrylamide concentration

FEBS Open Bio, EarlyView.
SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel, Qian He, Yixin Kang, Dianbao Zhang, Min Gao, Minglin Wang, Kexin Fan, Jingwen Xiong, Shaobo Wu, Botao Fa, Zhengtao Xiao, Yingfang Niu, Jun Yao, Yilei Zhang   +13 more
wiley   +1 more source

Deciphering the structural consequences of R83 and R152 methylation on DNA polymerase β using molecular modeling. [PDF]

PLoS One
Srivastava A, Idriss H, Das G, Abedrabbo S, Shamsir MS, Homouz D.   +5 more
europepmc   +1 more source

METTL3 knockout accelerates hepatocarcinogenesis via inhibiting endoplasmic reticulum stress response

FEBS Open Bio, EarlyView.
Liver‐specific knockout of N6‐methyladenosine (m6A) methyltransferase METTL3 significantly accelerated hepatic tumor initiation under various oncogenic challenges, contrary to the previously reported oncogenic role of METTL3 in liver cancer cell lines or xenograft models. Mechanistically, METTL3 deficiency reduced m6A deposition on Manf transcripts and
Bo Cui, Silin Tu, Haibo Li, Zhancheng Zeng, Ruiqi Xiao, Jing Guo, Xiaoqi Liang, Chang Liu, Lijie Pan, Wenjie Chen, Mian Ge, Xiaofen Zhong, Linsen Ye, Huaxin Chen, Qi Zhang, Yan Xu   +15 more
wiley   +1 more source

P745: High-fidelity whole genome sequencing of FFPE tumor-derived nucleic acids stored at ambient temperature

Genetics in Medicine Open
Farnoosh Abbas-Aghababazadeh, Lee Organick, Michael Blas, Benjamin Haibe-Kains, James Banal, Celeste Yu, Philippe Bedard, Heather Williams   +7 more
doaj