Results 351 to 360 of about 2,144,108 (379)

New DNA adducts of crotonaldehyde and acetaldehyde

Toxicology, 2001
This paper summarizes our recent studies on adducts produced in the reactions of the carcinogens crotonaldehyde (2-butenal) and acetaldehyde with deoxyguanosine (dG) and DNA. Human exposure to these carcinogens can be considerable, from both exogenous and endogenous sources.
Hecht, S. S., McIntee, Edward J., Wang, M.   +2 more
openaire   +2 more sources

Studies of chlorambucil—DNA adducts

Biochemical Pharmacology, 1992
Chlorambucil (CLB) is a bifunctional nitrogen mustard whose therapeutic and major side-effects are thought to be caused by binding to DNA. HPLC analysis of hydrolyzed DNA from L1210 cells incubated with [14C]CLB generated two peaks of radioactivity, indicating the formation of two or more major adducts.
openaire   +3 more sources

32P-Postlabeling Analysis of DNA Adducts

2014
32P-Postlabeling analysis is an ultra-sensitive method for the detection of DNA adducts, such as those formed directly by the covalent binding of carcinogens and mutagens to bases in DNA, and other DNA lesions resulting from modification of bases by endogenous or exogenous agents (e.g., oxidative damage).
Phillips, David H., Hewer, Alan, Arlt, Volker M.   +2 more
openaire   +7 more sources

DNA adducts and human atherosclerotic lesions

International Journal of Hygiene and Environmental Health, 2001
It has been hypothesized that mutational events may be involved in the atherogenetic process and that at least a portion of atherosclerotic plaques may be the results of monoclonal proliferation of a single mutated smooth muscle cell (SMC). Therefore, atherosclerosis may be similar to carcinogenesis and may have an environmental etiology.
Otta Boubelík, Přemysl Strejc, Irena Chvatalova, Blanka Binkova, Z. Stavkova, R. J. Sram   +5 more
openaire   +3 more sources

Characterization of oxaliplatin-DNA adduct formation in DNA and differentiation of cancer cell drug sensitivity at microdose concentrations.

Chemical Research in Toxicology, 2007
(trans-R, R)-1,2-diaminocyclohexaneoxalatoplatinum(II) (oxaliplatin) is a recently approved platinum analogue for use in the chemotherapy of metastatic colorectal cancer.
S. Hah, Rhoda A. Sumbad, R. de Vere White, K. Turteltaub, P. Henderson   +4 more
semanticscholar   +1 more source

Detection of carcinogen–DNA adducts by radioimmunoassay

Nature, 1977
COVALENT binding of carcinogen to nucleic acids is believed to be an essential component of the carcinogenic process1, so it is desirable to have highly sensitive and specific methods for detecting such adducts in cells and tissues exposed to known and suspected carcinogens.
Stuart H. Yuspa, M C Poirier, Blobstein S, I. B. Weinstein   +3 more
openaire   +3 more sources

Recognition of Platinum–DNA Adducts by HMGB1a

Biochemistry, 2012
Cisplatin (CP) and oxaliplatin (OX), platinum-based drugs used widely in chemotherapy, form adducts on intrastrand guanines (5'GG) in genomic DNA. DNA damage recognition proteins, transcription factors, mismatch repair proteins, and DNA polymerases discriminate between CP- and OX-GG DNA adducts, which could partly account for differences in the ...
Stephen G. Chaney, Brenda Temple, Nikolay V. Dokholyan, Srinivas Ramachandran, Anastassia N. Alexandrova   +4 more
openaire   +3 more sources

DNA Adduct Tolerance and Bypass

2000
The carrier ligands of platinum anticancer agents appear to play an important role in determining their efficacy against tumors with both intrinsic and acquired resistance to cisplatin. Unfortunately, both cisplatin and carboplatin have cis-diammine carrier ligands, and most cisplatin-resistant cell lines and tumors are cross-resistant to carboplatin ...
Alexandra Vaisman, Stephen G. Chaney
openaire   +2 more sources

Detection of DNA adducts by bioluminescence

SPIE Proceedings, 2001
Luminescent assay for detection ATP is very sensitive with limitation of 10-17 moles. ATP using styrene oxide as a model carcinogen we currently apply a luminescence technique to detect the very low levels of carcinogen-DNA adducts in vitro and in vivo.
Jian Chen, Xiang-Lin Tan, Min He, Shun-Qing Xu, Yi-Kai Zhou, Qun-Feng Yao   +5 more
openaire   +2 more sources

A synthetic nucleoside probe that discerns a DNA adduct from unmodified DNA.

Journal of the American Chemical Society, 2007
Selective pairing of engineering nucleosides in DNA duplexes provides a potential means to probe structurally modified DNA bases (i.e., DNA adducts) and address challenges associated with correlating adduct chemical structure with biological impact.
J. Gong, S. Sturla
semanticscholar   +1 more source