Results 261 to 270 of about 53,252 (284)

Measurement of a Malondialdehyde‐DNA Adduct

Current Protocols in Toxicology, 1999
AbstractDetermining the levels of various DNA adducts has become an essential tool in understanding the toxicology of carcinogens. Direct measurement of DNA adduct levels, the true biologically effective dose of a mutagen, can be correlated with biological outcomes or used to probe mechanisms of adduct formation.
Lawrence J. Marnett, John P. Plastaras
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Gold nanoprobes for detecting DNA adducts

Chem. Commun., 2014
Incorporating a modified nucleobase in oligomers coupled to gold nanoparticles enables sequence specific detection of a DNA adduct.
Ioannis A. Trantakis, Shana J. Sturla
openaire   +3 more sources

Methods for the Detection of DNA Adducts

2011
The detection and characterisation of DNA adducts can provide mechanistic information on mode of action for genotoxic chemicals and in this context is vital for human risk assessments. Adducts are measured extensively in biomonitoring studies to examine exposure to environmental, dietary, and occupational chemicals and as biomarkers of efficacy for ...
openaire   +4 more sources

New DNA adducts of crotonaldehyde and acetaldehyde

Toxicology, 2001
This paper summarizes our recent studies on adducts produced in the reactions of the carcinogens crotonaldehyde (2-butenal) and acetaldehyde with deoxyguanosine (dG) and DNA. Human exposure to these carcinogens can be considerable, from both exogenous and endogenous sources.
Hecht, S. S., McIntee, Edward J., Wang, M.   +2 more
openaire   +2 more sources

Studies of chlorambucil—DNA adducts

Biochemical Pharmacology, 1992
Chlorambucil (CLB) is a bifunctional nitrogen mustard whose therapeutic and major side-effects are thought to be caused by binding to DNA. HPLC analysis of hydrolyzed DNA from L1210 cells incubated with [14C]CLB generated two peaks of radioactivity, indicating the formation of two or more major adducts.
openaire   +3 more sources

32P-Postlabeling Analysis of DNA Adducts

2014
32P-Postlabeling analysis is an ultra-sensitive method for the detection of DNA adducts, such as those formed directly by the covalent binding of carcinogens and mutagens to bases in DNA, and other DNA lesions resulting from modification of bases by endogenous or exogenous agents (e.g., oxidative damage).
Phillips, David H., Hewer, Alan, Arlt, Volker M.   +2 more
openaire   +7 more sources

Detection of carcinogen–DNA adducts by radioimmunoassay

Nature, 1977
COVALENT binding of carcinogen to nucleic acids is believed to be an essential component of the carcinogenic process1, so it is desirable to have highly sensitive and specific methods for detecting such adducts in cells and tissues exposed to known and suspected carcinogens.
Stuart H. Yuspa, M C Poirier, Blobstein S, I. B. Weinstein   +3 more
openaire   +3 more sources

DNA adducts and human atherosclerotic lesions

International Journal of Hygiene and Environmental Health, 2001
It has been hypothesized that mutational events may be involved in the atherogenetic process and that at least a portion of atherosclerotic plaques may be the results of monoclonal proliferation of a single mutated smooth muscle cell (SMC). Therefore, atherosclerosis may be similar to carcinogenesis and may have an environmental etiology.
Otta Boubelík, Přemysl Strejc, Irena Chvatalova, Blanka Binkova, Z. Stavkova, R. J. Sram   +5 more
openaire   +3 more sources

Recognition of Platinum–DNA Adducts by HMGB1a

Biochemistry, 2012
Cisplatin (CP) and oxaliplatin (OX), platinum-based drugs used widely in chemotherapy, form adducts on intrastrand guanines (5'GG) in genomic DNA. DNA damage recognition proteins, transcription factors, mismatch repair proteins, and DNA polymerases discriminate between CP- and OX-GG DNA adducts, which could partly account for differences in the ...
Stephen G. Chaney, Brenda Temple, Nikolay V. Dokholyan, Srinivas Ramachandran, Anastassia N. Alexandrova   +4 more
openaire   +3 more sources

DNA Adduct Tolerance and Bypass

2000
The carrier ligands of platinum anticancer agents appear to play an important role in determining their efficacy against tumors with both intrinsic and acquired resistance to cisplatin. Unfortunately, both cisplatin and carboplatin have cis-diammine carrier ligands, and most cisplatin-resistant cell lines and tumors are cross-resistant to carboplatin ...
Alexandra Vaisman, Stephen G. Chaney
openaire   +2 more sources