Results 61 to 70 of about 1,695,496 (296)

Evaluation of DNA barcoding reference databases for marine species in the western and central Pacific Ocean [PDF]

PeerJ
DNA barcoding is a widely used tool for species identification, with its reliability heavily dependent on reference databases. While the quality of these databases has long been debated, a critical knowledge gap remains in their comprehensive evaluation ...
Yufei Zhou, Alejandro Trujillo-González, Simon Nicol, Roger Huerlimann, Stephen D. Sarre, Dianne Gleeson   +5 more
doaj   +2 more sources

DNA barcoding of Messor ants of Bulgaria with insights into their taxonomic diversity

Biodiversity Data Journal
Despite ongoing efforts to catalogue European ant species, studies focusing on the genetic diversity of Balkan ants remain limited. An integrative approach combining morphology, genetics, ecology and biogeography is preferable for accurately identifying species and resolving taxonomic uncertainties, particularly amongst challenging
Albena Lapeva-Gjonova, Monika Pramatarova, Lech Borowiec, Ilia Gjonov, Rumyana Kostova, Rostislav Bekchiev, Simeon Borissov   +6 more
openaire   +2 more sources

DNA barcoding of plants: Selection of core markers for taxonomic groups

Plant Science Today, 2017
Plant identification is a crucial and routine taxonomic procedure in order to understand and conserve the biodiversity. Anthropogenic activity, pollution, deforestation, and exploitation of natural resources have been threatening to the plant biodiversity.
Ankush Ashok Saddhe, Kundan Kumar
openaire   +2 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Evaluating the Utility of Single-Locus DNA Barcoding for the Identification of Ribbon Worms (Phylum Nemertea). [PDF]

PLoS ONE, 2016
Whereas many nemerteans (ribbon worms; phylum Nemertea) can be identified from external characters if observed alive, many are still problematic. When it comes to preserved specimens (as in e.g.
Per Sundberg, Sebastian Kvist, Malin Strand   +2 more
doaj   +1 more source

Taxonomic rank of Indian tortoise: revisit with DNA barcoding perspective

DNA Barcodes, 2013
Taxonomic rank of terrestrial tortoise inhabiting in India and adjacent countries has been an unresolved issue. Wild living species of Manouria and Indotestudo genera have been classiied through conventional taxonomy, but not adequately discriminated by mitochondrial cytochrome b gene.
Shantanu Kundu, Kulendra Chandra Das, Sankar Kumar Ghosh   +2 more
openaire   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

A taxonomically reliable DNA barcode reference library for North Sea macrobenthos

Scientific Data
Abstract EU directives (e.g. MSFD, Habitats Directive), along with OSPAR guidelines, mandate sustainable marine resource management across national borders. Benthic organisms are crucial for assessing marine ecosystem health, but their morphological identification is time-consuming and costly.
Magdalini Christodoulou, Sofie Derycke, Kevin K. Beentjes, Hans Hillewaert, Silke Laakmann, Kennet Lundin, Elham Kamyab, Sahar Khodami, Sara Maes, Henning Reiss, Carolin Uhlir, Laure Van den Bulcke, Berry Van der Hoorn, Annelies De Backer, Pedro Martinez Arbizu   +14 more
openaire   +4 more sources

Taxonomic, DNA Barcoding and Phylogenetic Reassessment of the Egyptian Ephedra L. (Ephedraceae) [PDF]

Catrina: The International Journal of Environmental Sciences, 2018
This study attempts to carry out a critical revision for the genus Ephedra in Egypt based on morpholo-gical characters for leaf, stem and flower. DNA sequencing data used for the first time for DNA barcoding of the family Ephedraceae in Egypt. One chloroplast marker-intergenic spacer trn-H/psb-A and another nuclear Internal Transcribed Spacer (ITS ...
Ahmed Faried, Ahmed EL-Banhawy, Mesfer Elqahtani   +2 more
openaire   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source