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The current status and trends of DNA extraction
Bioessays, 2023DNA extraction, playing an irreplaceable role in molecular biology as it is an essential step prior to various downstream biological analyses. Thus, the accuracy and reliability of downstream research outcomes depend largely on upstream DNA extraction ...
Xiaojun Ye, Bo Lei
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Journal of Natural Resources and Life Sciences Education, 2003
Genetic engineers must understand the structure and function of DNA in order to produce transgenic plants. This lesson describes DNA, how DNA relates to genes and chromosomes, and how and why DNA is extracted as a part of the genetic engineering process.
Hain, Patricia, Lee, Don
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Genetic engineers must understand the structure and function of DNA in order to produce transgenic plants. This lesson describes DNA, how DNA relates to genes and chromosomes, and how and why DNA is extracted as a part of the genetic engineering process.
Hain, Patricia, Lee, Don
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2018
Abstract Chapter 5 “DNA extraction” focuses on the particularities and practical constraints associated with the isolation of eDNA from environmental samples. The extraction protocol is indeed crucial in eDNA studies, as it will determine whether extracellular, intracellular, or total DNA is targeted.
Pierre Taberlet +3 more
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Abstract Chapter 5 “DNA extraction” focuses on the particularities and practical constraints associated with the isolation of eDNA from environmental samples. The extraction protocol is indeed crucial in eDNA studies, as it will determine whether extracellular, intracellular, or total DNA is targeted.
Pierre Taberlet +3 more
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Phytoplasma Plasmid DNA Extraction
2012Phytoplasma plasmids have generally been detected from DNA extracted from plants and insects using methods designed for the purification of total phytoplasma DNA. Methods include extraction from tissues that are high in phytoplasma titre, such as the phloem of plants, with the use of CsCl-bisbenzimide gradients that exploit the low G+C content of ...
Mark T, Andersen, Lia W, Liefting
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Comparison of DNA extraction methods from Geranium (Geraniaceae)
Acta Botanica Hungarica, 2019The genus Geranium (Geraniaceae); with about 320 species throughout the temperate regions, is chemically characterised by the presence of tannins, flavonoids, anthocyanins and essential oils which interfere with the extraction of pure genomic DNA.
S. Esfandani-Bozchaloyi +2 more
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DNA Extraction from Activated Sludges
Current Microbiology, 1999To optimize the cell lysis step for DNA extraction from activated sludge samples, two floc dispersion methods (sonication versus stirring with a cation exchange resin), and three cell lysis treatments (lysozyme + SDS, sonication in a water bath, and thermal shock) were tested.
M, Bourrain +3 more
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DNA Extraction, Preservation, and Amplification
2012The effectiveness of DNA barcoding as a routine practice in biodiversity research is strongly dependent on the quality of the source material, DNA extraction method, and selection of adequate primers in combination with optimized polymerase chain reaction (PCR) conditions.
Thomas, Knebelsberger, Isabella, Stöger
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Caulimovirus Isolation and DNA Extraction
1998Members of the cauhmovn-us group (1) each have a circular double-stranded DNA genome of approx 8 kbp that is encapsidated m a spherical, naked nucleocapsid of approx 50 nm diameter (Fig. 1). Caulimovnuses charactertsttcally produce subcellular mclusron bodies m infected tissues that contam most of the virions found in cells, embedded m an apparently ...
S N, Covey +3 more
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Manual Silica-Based DNA Extractions
2023There are several silica-based extraction methods that utilize silica-packed columns or silica-coated paramagnetic resin and are suitable for the needs of forensic DNA analysis and/or human identification. These rely on the use of chaotropic salts to alter the affinity of DNA such that it binds strongly to silica.
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Extraction of DNA from Paleofeces
2011Paleofeces are the nonmineralized remains of dung from extant and extinct fauna. They represent a surprisingly large proportion of fossil remains recovered from cave sites across the world. Paleofeces contain the DNA of the defecator as well as the DNA of ingested plant and animal remains.
Melanie, Kuch, Hendrik, Poinar
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