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2010
Publisher Summary This chapter focuses on the DNA extraction process. DNA molecules must be separated from other cellular material before they can be examined. Cellular proteins that package and protect DNA in the environment of the cell can inhibit the ability to analyze the DNA.
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Publisher Summary This chapter focuses on the DNA extraction process. DNA molecules must be separated from other cellular material before they can be examined. Cellular proteins that package and protect DNA in the environment of the cell can inhibit the ability to analyze the DNA.
openaire +2 more sources
2008
The isolation of genomic DNA is a fundamental requirement for many analytical molecular biology procedures. Although proper collection and stabilisation of the sample is crucial, purification of the DNA is often the key step for success in downstream analysis.
Ginny C. Saunders, Jennifer M. Rossi
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The isolation of genomic DNA is a fundamental requirement for many analytical molecular biology procedures. Although proper collection and stabilisation of the sample is crucial, purification of the DNA is often the key step for success in downstream analysis.
Ginny C. Saunders, Jennifer M. Rossi
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Extraction and Precipitation of
AbstractThis appendix presents protocols for phenol extraction and ethanol precipitation, methods that are routinely used to isolate DNA from biological sources or enzymatic reaction mixtures, to concentrate DNA samples, and to change from one solvent system to another.
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FEMS Microbiology Ecology, 2018
With the growing application of high‐throughput sequencing‐based metagenomics for profiling antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs), comparison of sample pretreatment and DNA extraction methods are needed to move toward ...
An-dong Li +8 more
semanticscholar +1 more source
With the growing application of high‐throughput sequencing‐based metagenomics for profiling antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs), comparison of sample pretreatment and DNA extraction methods are needed to move toward ...
An-dong Li +8 more
semanticscholar +1 more source
Plant Molecular Biology Reporter, 1997
S. Porebski, L. Bailey, B. Baum
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S. Porebski, L. Bailey, B. Baum
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DNA Extraction from Fossil Eggshell
2011Avian eggshell fragments recovered from both paleontological and archaeological deposits contain a cache of well-preserved ancient DNA. Here, we describe an extraction protocol that has been optimized to maximize the recovery of ancient DNA from fossil eggshell and minimize the co-purification of PCR inhibitors.
Oskam, C.L., Bunce, M.
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, 2003