Results 301 to 310 of about 2,395,071 (348)

Further characterization of DNA helicase activity of mouse DNA-dependent adenosine triphosphatase B (DNA helicase B)

Biochemistry, 1988
The DNA helicase activity of DNA-dependent ATPase B purified from mouse FM3A cells [Seki, M., Enomoto, T., Hanaoka, F., & Yamada, M. (1987) Biochemistry 26, 2924-2928] has been further characterized. The helicase activity was assayed with partially duplex DNA substrates in which oligonucleotides to be released by the enzyme were radiolabeled ...
M, Seki, T, Enomoto, J, Yanagisawa, F, Hanaoka, M, Ui   +4 more
openaire   +2 more sources

Enhancing helicase-dependent amplification by fusing the helicase with the DNA polymerase

Gene, 2008
In this study, we have engineered a new bifunctional protein named "helimerase", by physically linking Thermoanaerobacter tengcongensis UvrD helicase (TteUvrD) and Bacillus stearothermophilus DNA polymerase I Large Fragment (Bstpol) using a coiled-coil.
Aurélie, Motré, Ying, Li, Huimin, Kong
openaire   +2 more sources

Fluorometric assays for characterizing DNA helicases

Methods, 2010
DNA helicases belong to an important class of motor proteins and are involved in almost all aspects of DNA metabolism. They hydrolyze NTP to translocate along ssDNA and unwind dsDNA by relying on chemical to physical energy transfer processes that are achieved via nucleotide-state-dependent conformational changes.
Dou, Shuo-Xing, Xi, Xu Guang
openaire   +2 more sources

[DNA helicases and human diseases].

Medecine sciences : M/S, 2006
DNA helicases are molecular motors that catalyse the unwinding of energetically unstable structures into single strands and have therefore an essential role in nearly all metabolism transactions. Defects in helicase function can result in human syndromes in which predisposition to cancer and genomic instability are common features.
Uhring, Muriel, Poterszman, Arnaud
openaire   +2 more sources

Functions of DNA Helicases in the DNA Metabolism of Escherichia Coli

1984
DNA helicases catalyze the separation of double-stranded DNA into single strands using the energy of ATP hydrolysis. The four helicases which have been found in Escherichia coli are listed in Table 1. These enzymes are the helicases I, II, III and the helicase specified by the rep gene.
Abdel-Monem, M., Arthur , H., Benz, I., Hoffmann-Berling, H., Reygers, U., Seiter, A., Taucher-Scholz, G.   +6 more
openaire   +3 more sources

DNA HELICASES

Annual Review of Biochemistry, 1990
S W, Matson, K A, Kaiser-Rogers
openaire   +3 more sources

DNA Helicases of Escherichia coli

1991
A great deal has been learned in the last 15 years with regard to how helicase enzymes participate in DNA metabolism and how they interact with their DNA substrates. However, many questions remain unanswered. Of critical importance is an understanding of how NTP hydrolysis and hydrogen-bond disruption are coupled.
openaire   +2 more sources

XPB: An unconventional SF2 DNA helicase

Progress in Biophysics and Molecular Biology, 2015
XPB is a 3'-5' DNA helicase belonging to the superfamily 2 (SF2) of helicases. XPB is an essential core subunit of the eukaryotic basal transcription factor complex TFIIH which plays a dual role in transcription and DNA repair: 1) to facilitate the melting of the promoter during the initiation of RNA polymerase II transcription; 2) to unwind double ...
Li, Fan, Kevin T, DuPrez
openaire   +2 more sources

Molecular Characterization of Nuclear DNA Helicase II (RNA Helicase A)

2009
Nuclear DNA helicase II (NDH II) was first isolated from calf thymus using a DNA-unwinding assay. Subsequently it has been shown to be a homologue of human RNA helicase A (RHA) and the maleless protein (MLE) from Drosophila. Accordingly, the protein possesses both DNA and RNA unwinding activities.
Suisheng, Zhang, Frank, Grosse
openaire   +2 more sources

DNA Helicases in NER, BER, and MMR

2012
Different DNA repair mechanisms have evolved to protect our genome from modifications caused by endogenous and exogenous agents, thus maintaining the integrity of the DNA. Helicases often play a central role in these repair pathways and have shown to be essential for diverse tasks within these mechanisms. In prokaryotic nucleotide excision repair (NER)
Jochen, Kuper, Caroline, Kisker
openaire   +2 more sources