Results 101 to 110 of about 226,967 (304)

Efficient assembly of very short oligonucleotides using T4 DNA Ligase

BMC Research Notes, 2010
Background In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short ...
Holt Robert A, Coope Robin JN, Horspool Daniel R   +2 more
doaj   +1 more source

DNA damage induces Yap5-dependent transcription of ECO1/CTF7 in Saccharomyces cerevisiae.

PLoS ONE, 2020
Yeast Eco1 (ESCO2 in humans) acetyltransferase converts chromatin-bound cohesins to a DNA tethering state, thereby establishing sister chromatid cohesion.
Michael G Mfarej, Robert V Skibbens
doaj   +1 more source

The radioautograph of oligo 11 phosphorylated by T4 DNA ligase.

, 2012
The oligo 11 was phosphorylated by using commercial T4 DNA ligase. The phosphorylation products were loaded on a 15% denaturing PAGE gel (10×10×0.03 cm, A:B  = 29∶1, 7 M urea, 0.5 x TBE).
Wei Li (7081), Feng Gao (3548), Xuerong Zhang (155368), Huafu Zhou (155353)   +3 more
core   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

T4 DNA Ligase Reaction v2

, 2017
This is the reaction for the "Ligation Protocol with T4 DNA Ligase"

core   +1 more source

Mammalian DNA ligases. Biosynthesis and intracellular localization of DNA ligase I.

Journal of Biological Chemistry, 1990
Mammalian DNA ligase I is presumed to act in DNA replication. Rabbit antibodies against the homogeneous enzyme from calf thymus inhibited DNA ligase I activity and consistently recognized a single polypeptide of 125 kDa when cells from an established bovine kidney cell line (MDBK) were lysed rapidly by a variety of procedures and subjected to ...
D D, Lasko, A E, Tomkinson, T, Lindahl
openaire   +2 more sources

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

DNA ligase I from Xenopus laevis eggs.

, 1991
International audienceWe have purified the major DNA ligase from Xenopus laevis eggs and raised antibodies against it. Estimates from SDS PAGE indicate that this DNA ligase is a 180 kDa protein.
Aoufouchi, Saïd, Hardy, Serge, Prigent, Claude, Thiebaud, Pierre   +3 more
core   +1 more source

Water-soluble version of SCR7-pyrazine inhibits DNA repair and abrogates tumor cell proliferation

Journal of Radiation and Cancer Research, 2019
Aim: Mammalian DNA Ligases play pivotal role in processes such as DNA replication, recombination, and repair, which qualifies them as potent therapeutic targets to eradicate cancer cells.
Monica Pandey, Vidya Gopalakrishnan, Hassan A Swarup, Sujeet Kumar, Radha Gudapureddy, Anjana Elizabeth Jose, Supriya V Vartak, Robin Sebastian, Mrinal Srivastava, Bibha Choudhary, Mantelingu Kempegowda, Subhas S Karki, Sathees C Raghavan   +12 more
doaj   +1 more source

T4 DNA Ligase Reaction v1

, 2014
This is the reaction for the "Ligation Protocol with T4 DNA Ligase"

core   +1 more source