Results 151 to 160 of about 226,967 (304)

A Commentary on Mitochondrial Dysfunction and Compromised DNA Repair in Neurodegeneration: The Emerging Role of FUS in ALS

Neuroscience Insights
Mitochondrial dysfunction plays a pivotal role in the progression of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Alzheimer’s, and Parkinson’s disease.
Manohar Kodavati, Muralidhar L Hegde
doaj   +1 more source

Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination

, 1995
Three distinct DNA ligases, I to III, have been found previously in mammalian cells, but a cloned cDNA has been identified only for DNA ligase I, an essential enzyme active in DNA replication. A short peptide sequence conserved close to the C terminus of
Schar, P., Shell, B. K., Wang, R. P., Wei, Y. F., Yu, G. L., Carter, K., Robins, P., Caldecott, K., Pappin, D. J., Nash, R. A.   +9 more
core  

Ligase-Mediated Threose Nucleic Acid Synthesis on DNA Templates

, 2019
Ligases are a class of enzymes that catalyze the formation of phosphodiester bonds between an oligonucleotide donor with a 5′ terminal phosphate and an oligonucleotide acceptor with a 3′ terminal hydroxyl group.
Jen-Yu Liao (4036217), Cailen M. McCloskey (6203723), John C. Chaput (29064), Saikat Bala (2396920)   +3 more
core   +1 more source

Purification and characterization of DNA ligase III from bovine testes. Homology with DNA ligase II and vaccinia DNA ligase.

The Journal of biological chemistry, 1995
Mammalian cell nuclei contain three biochemically distinct DNA ligases. In the present study we have found high levels of DNA ligase I and DNA ligase III activity in bovine testes and have purified DNA ligase III to near homogeneity. The high level of DNA ligase III suggests a role for this enzyme in meiotic recombination.
I, Husain, A E, Tomkinson, W A, Burkhart, M B, Moyer, W, Ramos, Z B, Mackey, J M, Besterman, J, Chen   +7 more
openaire   +1 more source

Combining Fret and Super‐Resolution Microscopy Reveals Kinase Activation and Mitochondrial Activity at the Nanoscale

Advanced Science, EarlyView.
BioSenSRRF is an open‐source workflow that combines conventional FRET biosensors with SRRF reconstruction to generate sub‐diffraction FRET index maps on standard microscopes. The pipeline integrates automated image registration, SRRF reconstruction, quantitative FRET index calculation, and random line‐based hotspot analysis to uncover AURKA‐dependent ...
Nicolas Y. Jolivet, Pierre‐Jean Desmaison, Xavier Pinson, Arthur Masson, Olivier Delalande, Giulia Bertolin   +5 more
wiley   +1 more source

Tuning T4 DNA ligase action within self-assembled DNA nanostructures

, 2022
The enzymatic ligation of DNA is a vital reaction in all living organisms, covalently joining fragmented DNA molecules during DNA replication, repair and recombination processes, and is fundamental for recombinant DNA technologies.
Aboagye-Mensah, Dorothy
core  

Metabolic Imbalance Triggers Adaptive Remodeling to Accelerate Diploidization in Murine Haploid Embryonic Stem Cells

Advanced Science, EarlyView.
In this article, Shuai and colleagues demonstrate that metabolic remodeling drives self‐diploidization in murine haploid ESCs (haESCs). Mitochondrial dysfunction and imbalanced pyruvate metabolism underlie this process. Genome‐wide screening using haESCs identifies key mitochondrial quality‐control related genes, enabling a metabolism‐based medium that
Yi Fu, Wenhao Zhang, Yifan Zhang, Yu He, Yi Du, Yiding Zhao, Chunmeng Yao, Shengyi Sun, Xiaoyan Sheng, Qian Gao, Chao Tong, Ling Shuai   +11 more
wiley   +1 more source

MG53 Coordinates Macrophage Polarization and Neuroimmune Coupling to Promote Corneal Nerve Regeneration via the MPEG1–MVP–STAT6 Axis

Advanced Science, EarlyView.
Corneal nerve regeneration is critical to corneal wound healing processes. The current study reveals a novel role of MG53 in promoting corneal nerve regeneration after alkali induced injury. Mechanistically, MG53 enters macrophages via its receptor, MPEG1, promotes MVP K63 ubiquitination, and triggers STAT6 induced repair‐related genes expression ...
Peng Chen, Zhentao Zhang, Lilian Sakai, Yanping Xu, Luxi Zhang, Han Ye, William Sollenberger, Takahide Ikeda, Zhiyu Yan, Keerthika Sathish, Usman Alizai, Haitao Wen, Timothy M. Pawlik, Heather L. Chandler, Hua Zhu   +14 more
wiley   +1 more source

The Trichinella Super‐Pangenome Reveals the Evolution of Encapsulation and Predicted Host–Parasite Protein Interactions

Advanced Science, EarlyView.
ABSTRACT The muscle capsule of Trichinella is a critical structure that impedes immune attacks and drug penetration, yet the molecular mechanisms underlying its formation remain poorly understood. Using a high‐quality super‐pangenome comprising 12 Trichinella species, we compared extensive genomic variations between encapsulating and non‐encapsulating ...
Qingbo Lv, Yi Liu, Ning Xu, Xiao Zhang, Yaming Yang, Chengyao Li, Hanhai Mao, Mingyuan Liu, Xiaolei Liu   +8 more
wiley   +1 more source

ESCRT‐Mimetic Nanodegrader Targets STING for Anti‐Inflammatory Therapy

Advanced Science, EarlyView.
A nanoplatform‐enabled targeted protein degradation strategy is presented to regulate aberrant STING signaling. STING‐ATTEC induces selective autophagic degradation of STING via formation of a STING–ATTEC–LC3 ternary complex, while the cationic FA‐LNP+ system enhances LC3 generation and targeted delivery. Together, this synergistic approach efficiently
Fuyuan Zhou, Qiming Zhai, Zhihao Yao, Qing Li, Yan Yang, Si Wang, Chao Huang, Liangjing Xin, Tao Chen, Jinlin Song   +9 more
wiley   +1 more source