Results 211 to 220 of about 77,074 (235)
Some of the next articles are maybe not open access.
The α-thio and/or β-γ-hypophosphate analogs of ATP as cofactors of T4 DNA ligase
Bioorganic Chemistry, 2016T4 DNA ligase is one of the most commonly used enzymes for in vitro molecular research and a useful model for testing the ligation mechanism of ATP-dependent DNA ligation. To better understand the influence of phosphate group modifications in the ligation process, a series of ATP analogs were tested as cofactors.
Roza Pawlowska +2 more
exaly +3 more sources
A sensitive ligase-based ATP electrochemical assay using molecular beacon-like DNA
Biosensors and Bioelectronics, 2010A sensitive and selective ligase-based signal-on electrochemical sensing method for adenosine-5'-triphosphate (ATP) detection had been developed using molecular beacon (MB)-like DNA. In this method, the biotin-tagged MB-like DNA was self-assembled onto a gold electrode to form a stem-loop structure by means of facile gold-thiol chemistry, which ...
Yong-hong Wang, Xiaoxiao He
exaly +3 more sources
Extremophiles, 2002
A gene encoding a putative ATP-dependent DNA ligase was identified in the genome of the hyperthermophilic archaeon Sulfolobus shibatae and expressed in Escherichia coli. The 601 amino acid recombinant polypeptide was a monomeric protein capable of strand joining on a singly nicked DNA substrate in the presence of ATP ( K(m)=34 micro mu) and a divalent ...
Xiaoqin, Lai +3 more
exaly +3 more sources
A gene encoding a putative ATP-dependent DNA ligase was identified in the genome of the hyperthermophilic archaeon Sulfolobus shibatae and expressed in Escherichia coli. The 601 amino acid recombinant polypeptide was a monomeric protein capable of strand joining on a singly nicked DNA substrate in the presence of ATP ( K(m)=34 micro mu) and a divalent ...
Xiaoqin, Lai +3 more
exaly +3 more sources
Angewandte Chemie - International Edition
Abstract Discriminating against base‐pair mismatches by nucleic acid‐related enzymes is essential for DNA replication, RNA replication, nucleic acid repair, transcription, and translation. However, the discrimination mechanisms at the atomic level remain poorly understood, limited by the availability of atom ...
Bei Hu, Zhen Huang
exaly +3 more sources
Abstract Discriminating against base‐pair mismatches by nucleic acid‐related enzymes is essential for DNA replication, RNA replication, nucleic acid repair, transcription, and translation. However, the discrimination mechanisms at the atomic level remain poorly understood, limited by the availability of atom ...
Bei Hu, Zhen Huang
exaly +3 more sources
Functional domains of an ATP-dependent DNA ligase 1 1Edited by A. R. Fersht
Journal of Molecular Biology, 1999Dale B Wigley
exaly +2 more sources
Mechanistic and Kinetic Study of the ATP-Dependent DNA Ligase of Neisseria meningitidis
Biochemistry, 2003The gene from Neisseria meningitidis serogroup A, encoding a putative, secreted ATP-dependent DNA ligase was cloned and overexpressed, and the soluble protein was purified. Mass spectrometry indicated that the homogeneous protein was adenylated as isolated, and sedimentation velocity experiments suggested that the enzyme exists as a monomer in solution.
Sophie, Magnet, John S, Blanchard
openaire +2 more sources
Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana
Plant Molecular Biology, 2001Here we report the purification and biochemical characterization of recombinant Arabidopsis thaliana DNA ligase I. We show that this ligase requires ATP as a source for adenylation. The calculated Km [ATP] for ligation is 3 microM. This enzyme is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates, but not in oligo(dT)/poly(rA)
Y Q, Wu, B, Hohn, A, Ziemienowic
openaire +2 more sources
International Journal of Biological Macromolecules, 2023
The genome sequence of hyperthermophilic archaeon Pyrobaculum calidifontis contains an open reading frame, Pcal_0039, which encodes a putative DNA ligase. Structural analysis disclosed the presence of signature sequences of ATP-dependent DNA ligases. We have heterologously expressed Pcal_0039 gene in Escherichia coli.
Qamar, Abbas +4 more
openaire +2 more sources
The genome sequence of hyperthermophilic archaeon Pyrobaculum calidifontis contains an open reading frame, Pcal_0039, which encodes a putative DNA ligase. Structural analysis disclosed the presence of signature sequences of ATP-dependent DNA ligases. We have heterologously expressed Pcal_0039 gene in Escherichia coli.
Qamar, Abbas +4 more
openaire +2 more sources
Free-labelled fluorescent method for ATP detection assisted by T4 DNA ligase
Analytical Methods, 2017A facile, highly selective biosensor was used for ATP quantitative analysis.
Hongyun Huang +5 more
openaire +1 more source