Results 221 to 230 of about 77,074 (235)

Functional domains of an ATP-dependent DNA ligase.

Journal of molecular biology, 1999
The crystal structure of an ATP-dependent DNA ligase from bacteriophage T7 revealed that the protein comprised two structural domains. In order to investigate the biochemical activities of these domains, we have overexpressed them separately and purified them to homogeneity. The larger N-terminal domain retains adenylation and ligase activities, though
A J, Doherty, D B, Wigley
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Synthesis and bioevaluation of aryl hydroxamates distinguishing between NAD+ and ATP-dependent DNA ligases

MedChemComm, 2012
Identification of compounds distinguishing between NAD+ and ATP-dependent ligases is a key factor to discover new antimicrobials. Based on virtual screening experiments a series of hydroxamates distinguishing between the above two classes of enzymes are synthesized.
Vandna Kukshal, Mridul Mishra, Arya Ajay, Taran Khanam, Rahul Sharma, Divya Dube, Deepti Chopra, Rama Pati Tripathi, Ravishankar Ramachandran   +8 more
openaire   +1 more source

A new group of plant-specific ATP-dependent DNA ligases identified by protein phylogeny, hydrophobic cluster analysis and 3-dimensional modelling

Functional Plant Biology, 2005
The eukaryotic ATP-dependent DNA ligases comprise a group of orthologous proteins that have distinct roles in DNA metabolism. In contrast with the well-known DNA ligases of animal cells, the DNA ligases of plant cells are poorly described. Until now, only two DNA ligases (I and IV) genes of Arabidopsis thaliana (L.) Heynh were isolated and ...
Diego, Bonatto, Martin, Brendel, João Antonio Pêgas, Henriques   +2 more
openaire   +2 more sources

Interaction of β-L-Adenosine-5′-triphosphate (L-ATP) with Human Deoxycytidine Kinase, Human DNA Primase and T4 DNA Ligase: Does the Chance Direct Enzymatic Enantioselectivity?

Nucleosides and Nucleotides, 1999
We demonstrate that L-ATP: 1) as well as its natural D-enantiomer, acts as a phosphate donor in the reaction catalysed by human deoxycytidine kinase; 2) inhibits human DNA-primase and the ATP-dependent T4 DNA ligase. Thus, the lack of enantioselectivity of the enzymes is more frequent than it was believed a few years ago and we suggest that it would ...
A, Verri, A, Montecucco, G, Gosselin, V, Boudou, S, Spadari, J L, Imbach, F, Focher   +6 more
openaire   +2 more sources

ATP-type DNA ligase requires other proteins for its activity in vitro and its operon components for radiation resistance in Deinococcus radiodurans in vivo

Biochemistry and Cell Biology, 2010
A multiprotein DNA processing complex isolated from Deinococcus radiodurans contains the DNA repair protein PprA, an ATP-type DNA repair ligase (LigB) encoded by the drB0100 gene, and protein kinase activity. An ATP-dependent DNA end-joining activity was detected in the complex.
Swathi, Kota, Vidya A, Kamble, Yogendra S, Rajpurohit, Hari S, Misra   +3 more
openaire   +2 more sources

Interaction of beta-L-adenosine-5 '-triphosphate (L-ATP) with human deoxycytidine kinase, human DNA primase and T4 DNA ligase: Does the chance direct enzymatic enantioselectivity?

1999
We demonstrate that L-ATP: 1) as well as its natural D-enantiomer, acts as a phosphate donor in the reaction catalysed by human deoxycytidine kinase; 2) inhibits human DNA-primase and the ATP-dependent T4 DNA ligase. Thus, the lack of enantioselectivity of the enzymes is more frequent than it was believed a few years ago and we suggest that it would ...
Verri A, Montecucco A, Gosselin G, Boudou V, Spadari S, Focher F   +5 more
openaire   +1 more source

ATP-dependent DNA ligase fromThermococcussp. 1519 displays a new arrangement of the OB-fold domain

Acta Crystallographica Section F: Structural Biology Communications, 2012
Tatiana E Petrova, Ekaterina Y Bezsudnova, Konstantin Boyko   +2 more
exaly  

ATP-dependent DNA ligase from Archaeoglobus fulgidus displays a tightly closed conformation

Acta Crystallographica Section F: Structural Biology Communications, 2009
Sang Jae Lee, Se Won Suh
exaly  

Characterization of Bacteriophage T3 DNA Ligase

Journal of Biochemistry, 2004
Liang Cai
exaly  

Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation

Nucleic Acids Research, 2018
Shih-Hsun Chen, Xiaochun Yu
exaly