ABSTRACT Monitoring marine biodiversity requires approaches capable of capturing its spatial and temporal complexity. DNA metabarcoding coupled with Autonomous Reef Monitoring Structures (ARMS) is increasingly used for this purpose, yet most applications still pool all sessile fractions and rarely benchmark molecular outputs against photographic ...
Anne Chenuil +22 more
wiley +1 more source
ABSTRACT Metabarcoding has become the method of choice for characterizing complex arthropod communities. The extent to which metabarcoded bulk samples will recover the same community composition as individual sequencing of all individuals in the sample remains poorly quantified.
Brendan Furneaux +14 more
wiley +1 more source
Analysis of Fish Diversity and Invasive Species in the Cuijiang River Based on Environmental DNA Metabarcoding. [PDF]
Li H +13 more
europepmc +1 more source
Surveying Biodiverse Coral Reef Ecosystems Across the Tree of Life With Environmental DNA
ABSTRACT A central challenge in evaluating biodiversity using environmental DNA (eDNA) metabarcoding is that each primer set has biases resulting in selective amplification of taxonomic diversity. Here we test 11 primer pairs to assess Central Pacific coral reef diversity in the five kingdoms of marine eukaryotes: Chromista, Fungi, Metazoa, Plantae and
Van Wishingrad +2 more
wiley +1 more source
Simultaneous identification of multiple thrips species (Thysanoptera: Thripidae) from sticky traps using DNA metabarcoding. [PDF]
Zeira E +6 more
europepmc +1 more source
Guidelines for standardization of DNA metabarcoding
Guidelines for standardization of DNA ...
openaire +1 more source
Getting to the Core of the Matter—Assessing the Role of Replication in Metabarcoding‐Based sedaDNA
ABSTRACT Replication is central to most experimental and sampling designs, increasing inferential power and capturing fine‐scale data heterogeneity. However, its importance remains poorly evaluated in some ecological and evolutionary settings. This is the case of metabarcoding studies using DNA recovered from sedimentary archives, in which biological ...
Elena Baños +7 more
wiley +1 more source
Comparing Accuracy and Biases of DNA Metabarcoding, Hybridization Capture, and Metagenomic Sequencing for Quantifying Herbivore Diets. [PDF]
Eriksson CE +3 more
europepmc +1 more source
Substrate‐Dependent Variation in Environmental DNA Persistence and Degradation From a Small Mammal
ABSTRACT Environmental DNA (eDNA) analyses have been applied to terrestrial environments for over a decade, yet knowledge of the dynamics of eDNA deposition, movement and degradation remains limited. To address this, we conducted a controlled experiment over 1 year to assess the persistence of eDNA from an arboreal mammal across four substrates (water,
Austin M. Guthrie +5 more
wiley +1 more source
Desiccation to DNA: Bulk DNA Metabarcoding of Macroinvertebrate Egg Banks via Sugar-Flotation Enhances Biodiversity Surveys of Dry Salt Lakes. [PDF]
Lawrie AD +10 more
europepmc +1 more source

