Results 111 to 120 of about 1,501,815 (329)
International Journal of Plant Biology, 2011 DNA polymerase is an enzyme that adds nucleotides to the growing DNA chain during replication and DNA repair. DNA polymerase activity and fidelity are important characteristics that reflect the ability of DNA polymerase to add nucleotides and then ...
Andrey Golubov +3 more
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Molecular Oncology, EarlyView.Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra +10 more
wiley +1 more source
DNA extraction and polymerase chain reaction
Journal of Cytology, 2019 DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
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Atm deficiency in the DNA polymerase β null cerebellum results in cerebellar ataxia and Itpr1 reduction associated with alteration of cytosine methylation [PDF]
, 2020 Jusik Kim +3 more
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Molecular Oncology, EarlyView.Generation of two normal and tumour (cancerous) paired human cell lines using an established tissue culture technique and their characterisation is described. Cell lines were characterised at cellular, protein, chromosome and gene expression levels and for HPV status.
Simon Broad +12 more
wiley +1 more source
COMPARISON OF SIX COMMERCIALLY-AVAILABLE DNA POLYMERASES FOR DIRECT PCR
Revista do Instituto de Medicina Tropical de São Paulo, 2013 SUMMARY The use of a “direct PCR” DNA polymerase enables PCR amplification without any prior DNA purification from blood samples due to the enzyme's resistance to inhibitors present in blood components. Such DNA polymerases are now commercially available.
Masashi Miura +3 more
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An interaction between replication protein A and SV40 T antigen appears essential for primosome assembly during SV40 DNA replication [PDF]
, 1993 Replication protein A from human cells (hRPA) is a multisubunit single-stranded DNA-binding protein (ssb) and is essential for SV40 DNA replication in vitro.
Melendy, T., Stillman, B.
core
, 2006 In this chapter, we would like to highlight the possible connections between DNA polymerases, the main enzymes in DNA metabolism, and human diseases, also critically evaluating those cases where the experimental data are not fully convincing. To this aim, we will first give a short overview of the three main DNA metabolic events, namely replication ...
Ramadan, K, Maga, G, Hübscher, U
openaire +2 more sources