Results 141 to 150 of about 945,929 (245)

Physico‐chemical study of complex formation of DNA with wild‐type and mutant E. coli RNA polymerases Recognition properties of β‐subunit [PDF]

, 1986
Olga N. Ozoline, S. G. Kamzolova
openalex   +1 more source

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

CRISPR-DNA Polymerase Assisted Targeted Mutagenesis for Regulable Laboratory Evolution. [PDF]

Adv Sci (Weinh)
Chen S, Chen X, Peng Y, Li Q, Zhou J, Li J, Du G, Chen J, Zhang G.   +8 more
europepmc   +1 more source

A Peptide Nucleic Acid‐Functionalized Heterojunction Thin Film Transistor as a Scalable and Reusable Platform for Label‐Free Detection of MicroRNA

Advanced Functional Materials, EarlyView.
A miniaturized, label‐free, and enzyme‐free biosensor (miR‐TFT) enables direct electrical detection of microRNA (miRNA) with single‐nucleotide specificity and a detection limit of 0.6 fM. Built on a tri‐channel In2O3/ZnO heterojunction and functionalized with bespoke peptide nucleic acid (PNA) probes, the device is robust, reusable, and compatible with
Wejdan S. Al Ghamdi, Jorge Romero Domínguez, Erol Hasan, Temur Maksudov, Thomas D. Anthopoulos, Dana Alsulaiman   +5 more
wiley   +1 more source

Noncompetitive Inhibition of DNA Polymerase β by a Nonnative Nucleotide. [PDF]

J Org Chem
Howlader AH, Jiang X, Zhou Z, Greenberg MM.   +3 more
europepmc   +1 more source

The S229L Colon Tumor-associated Variant of DNA Polymerase β Induces Cellular Transformation as a Result of Decreased Polymerization Efficiency [PDF]

, 2014
Antonia A. Nemec, Drew L. Murphy, Katherine A. Donigan, Joann B. Sweasy   +3 more
openalex   +1 more source

Microporous Microgel Assemblies Facilitating the Recruitment and Osteogenic Differentiation of Progenitor Cells for Bone Regeneration

Advanced Functional Materials, EarlyView.
There is a significant need for biomaterials with well‐defined stability and bioactivity to support tissue regeneration. In this study, we developed a tunable microgel platform that enables the decoupling of stiffness from porosity, thereby promoting bone regeneration.
Silvia Pravato, Haishuo Guan, Bianca M. Carrara, Mira Jacobs, Matteo Monticelli, Lukas Moser, Thomas Moragues, Andrew J. deMello, Tomaso Zambelli, Martin Ehrbar   +9 more
wiley   +1 more source

The non-catalytic DNA polymerase ε subunit is an NPF motif recognition protein. [PDF]

Nat Commun
Keskitalo S, Zambo B, Malaymar Pinar D, Tuhkala A, Salokas K, Turunen T, Deutsch N, Davey N, Dosztányi Z, Varjosalo M, Gogl G.   +10 more
europepmc   +1 more source

Harnessing Non‐Covalent Protein–Protein Interaction Domains for Production of Biocatalytic Materials Systems

Advanced Functional Materials, EarlyView.
Non‐covalent protein–protein interactions mediated by SH3, PDZ, or GBD domains enable the self‐assembly of stable and biocatalytically active hydrogel materials. These soft materials can be processed into monodisperse foams that, once dried, exhibit enhanced mechanical stability and activity and are easily integrated into microstructured flow ...
Julian S. Hertel, Maria Alessandra Martini, Marius Stoeckle, Annika J. Weber, Kersten S. Rabe, Christof M. Niemeyer   +5 more
wiley   +1 more source

Thermodynamic Profiling Reveals DNA Polymerase Template Binding, Substrate Incorporation, and Exonuclease Function. [PDF]

Int J Mol Sci
Sun Y, Lin W, Fu K, Gao J, Zhao X, He Y, Tian H.   +6 more
europepmc   +1 more source