Results 41 to 50 of about 812,933 (320)

The Escherichia coli polB Locus Is Identical to dinA, the Structural Gene for DNA Polymerase II

open access: yesJournal of Biological Chemistry, 1997
Escherichia coli DNA polymerase II (Pol II) is a member of the group B, “α-like” family of DNA polymerases. Pol II is encoded by the damage-inducible dinA gene and exhibits SOS induction under the control of Lex A repressor.
Z. Qiu, M. Goodman
semanticscholar   +1 more source

DNA polymerase III from Saccharomyces cerevisiae. II. Inhibitor studies and comparison with DNA polymerases I and II.

open access: yesJournal of Biological Chemistry, 1988
The newly identified yeast DNA polymerase III was compared to DNA polymerases I and II and the mitochondrial DNA polymerase. Inhibition by aphidicolin (I50) of DNA polymerases I, II, and III was 4, 6, and 0.6 micrograms/ml, respectively. The mitochondrial enzyme was insensitive to the drug.
Peter M. J. Burgers, G. A. Bauer
openaire   +3 more sources

DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1991
The Saccharomyces cerevisiae DNA polymerase II holoenzyme consists of five polypeptides. The largest is the catalytic subunit, whose gene (POL2) has been cloned and sequenced. Herein we describe the cloning and sequencing of DPB2, the gene for the second
H. Araki   +3 more
semanticscholar   +1 more source

Interactions of the Bacillus subtilis DnaE polymerase with replisomal proteins modulate its activity and fidelity [PDF]

open access: yesOpen Biology, 2017
During Bacillus subtilis replication two replicative polymerases function at the replisome to collectively carry out genome replication. In a reconstituted in vitro replication assay, PolC is the main polymerase while the lagging strand DnaE polymerase ...
Vasileios Paschalis   +5 more
doaj   +1 more source

RNA Polymerase II transcription independent of TBP in murine embryonic stem cells

open access: yeseLife, 2023
Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation ...
James ZJ Kwan   +8 more
doaj   +1 more source

Aphidicolin inhibits DNA polymerase II ofEscherichia coli, an alpha-like DNA polymerase

open access: yesNucleic Acids Research, 1990
The polB gene of E. coli encodes DNA polymerase II. We have reported the cloning (1,2) and sequencing of the polB gene (3) (GenBank Accession number M35371). DNA polymerase II appears to be a non-essential function since the polB gene is missing from some widely used strains (3). Several sub-families of DNA polymerases have been identified (4). In this
Robb E. Moses   +3 more
openaire   +3 more sources

A mutant of Escherichia coli defective in DNA polymerase II activity.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1972
A mutant of E. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to replicate DNA of various sources was analyzed.
Y. Hirota, M. Gefter, L. Mindich
semanticscholar   +1 more source

Transcription Factor Binding to Replicated DNA

open access: yesCell Reports, 2020
Summary: Genome replication perturbs the DNA regulatory environment by displacing DNA-bound proteins, replacing nucleosomes, and introducing dosage imbalance between regions replicating at different S-phase stages.
Raz Bar-Ziv   +3 more
doaj  

Establishing a Genetic Detection Protocol of Single Nucleotide Polymorphisms Panels in Inbred Rats Based on Multiplex PCR-LDR

open access: yesShiyan dongwu yu bijiao yixue, 2023
Objective To establish a set of single nucleotide polymorphisms (SNP) detection protocol for inbred rats based on multiplex PCR-ligase detection reaction (LDR).Methods A total of 40 rats SNP sites were selected on chromosomes 1-20 and X of rats among 5 ...
ZHAO Liya   +7 more
doaj   +1 more source

Monofunctional DNA-platinum(II) adducts block frequently DNA polymerases

open access: yesNucleic Acids Research, 1992
The question of whether monofunctional DNA platinum(II) adducts block synthesis of DNA by purified DNA polymerases of different types and origin has been investigated by comparing the time dependence of synthesis arrest and of DNA adduct formation.
Reinhard Bauer   +2 more
openaire   +4 more sources

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