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Historical Pandemic and Contemporary Influenza A Viruses Reveal PB2 M631L as a Convergent Adaptation to Human ANP32 [PDF]
MicroorganismsUnderstanding the genetic changes that allow avian influenza A viruses (IAVs) to switch their natural hosts and establish productive infection in humans is important for pandemic risk assessment. Adaptations in the IAV polymerase are required to overcome
Matthias Budt +5 more
doaj +2 more sources
Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair. Modern biotechnology and molecular diagnostics rely heavily on DNA polymerases in analyzing nucleic acids.
Maxim Filipenko, Igor Oscorbin
exaly +4 more sources
Frontiers in Bioengineering and Biotechnology, 2021 A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes +3 more
doaj +1 more source
A new contribution to Megasporoporia sensu lato: Six new species and three new combinations
Frontiers in Microbiology, 2022 Megasporoporia sensu lato has recently been intensively studied in China and South America, and four independent clades representing four genera have been recognized phylogenetically. In this study, more samples, mostly from subtropical and tropical Asia,
Ya-Rong Wang +7 more
doaj +1 more source
Viruses, 2021 Viruses have evolved numerous strategies to maximize the use of their limited genetic material, including proteolytic cleavage of polyproteins to yield products with different functions.
Dennis S. Winston, David D. Boehr
doaj +1 more source
Beyond the Lesion: Back to High Fidelity DNA Synthesis
Frontiers in Molecular Biosciences, 2022 High fidelity (HiFi) DNA polymerases (Pols) perform the bulk of DNA synthesis required to duplicate genomes in all forms of life. Their structural features, enzymatic mechanisms, and inherent properties are well-described over several decades of research.
Joseph D. Kaszubowski +1 more
doaj +1 more source
LigD: A Structural Guide to the Multi-Tool of Bacterial Non-Homologous End Joining
Frontiers in Molecular Biosciences, 2021 DNA double-strand breaks are the most lethal form of damage for living organisms. The non-homologous end joining (NHEJ) pathway can repair these breaks without the use of a DNA template, making it a critical repair mechanism when DNA is not replicating ...
Benhur Amare +10 more
doaj +1 more source
Are There Mutator Polymerases? [PDF]
The Scientific World JOURNAL, 2003 DNA polymerases are involved in different cellular events, including genome replication and DNA repair. In the last few years, a large number of novel DNA polymerases have been discovered, and the biochemical analysis of their properties has revealed a long list of intriguing features.
García Díaz, Miguel +4 more
openaire +6 more sources
Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides
Molecules, 2020 In the last three decades, oligonucleotides have been extensively investigated as probes, molecular ligands and even catalysts within therapeutic and diagnostic applications.
Steven Ochoa, Valeria T. Milam
doaj +1 more source
Viruses, 2020 Current influenza vaccines have modest efficacy. This is especially true for current live attenuated influenza vaccines (LAIV), which have been inferior to the inactivated versions in recent years.
Andrew Cox +7 more
doaj +1 more source